Atxn1 (NM_001199304) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2900016G23Rik; Atx1; C85907; ENSMUSG00000074917; Gm10786; Sca1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228941 representing NM_001199304 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATCCAACCAAGAGCGGAGCAACGAATGCCTGCCTCCCAAGAAACGTGAGATCCCCGCCACCAGCC GGCCCTCGGAGGAGAAGGCCACTGCTCTGCCCAGCGACAACCACTGCGTGGAGGGTGTGGCCTGGCTCCC CAGCACCCCTGGCATCCGCGGCCATGGGGGTGGGCGGCACGGGTCAGCAGGGACTTCCGGGGAGCATGGT TTACAAGGAATGGGTTTACATAAAGCACTGTCCGCAGGGCTGGATTACTCCCCACCCAGTGCCCCCAGGT CAGTCCCCACAGCCAACACGCTGCCCACCGTGTACCCTCCTCCTCAGTCAGGGACCCCGGTGTCTCCTGT GCAGTACGCCCACCTTTCGCATACCTTCCAGTTCATTGGGTCCTCCCAATACAGTGGGCCTTACGCGGGC TTTATCCCTTCCCAGCTGATCTCCCCATCAGGCAACCCGGTCACCAGTGCAGTAGCCTCAGCTGCAGGGG CCACCACTCCATCACAGCGCTCCCAGCTGGAGGCTTATTCCACCCTGCTGGCCAACATGGGCAGTCTGAG CCAGGCACCAGGACATAAGGTTGAGCCCCCTCCGCAGCAGCACCTCAGCAGGGCTGCAGGATTAGTCAAC CCGGGGTCCCCTCCTCCACCCACCCAGCAGAACCAGTACATCCATATTTCCAGCTCTCCACAGAGCTCCG GGCGGGCGACATCTCCCCCACCCATCCCGGTCCACCTCCATCCCCATCAGACGATGATCCCGCACACACT CACCCTGGGGCCTTCATCCCAGGTGGTTGTGCAATATAGTGATGCCGGAGGCCACTTTGTTCCTCGAGAG TCCACCAAAAAAGCCGAGAGCAGCAGGTTGCAGCAGGCTATGCAAGCCAAGGAAGTCCTGAATGGGGAGA TGGAGAAAAGCCGGAGGTATGGGGCATCATCTTCTGTGGAGCTGAGCCTAGGCAAGGCAAGCAGTAAGTC AGTGCCTCATCCCTATGAGTCCAGGCATGTGGTGGTCCACCCAAGCCCAGCAGACTACAGCAGTCGTGAT ACCTCCGGGGTCCGTGGATCTGTGATGGTTCTGCCTAATAGCAGCACACCCTCAGCCGACCTGGAGGCCC AGCAGACCACGCATCGAGAGGCCTCCCCATCCACCCTCAATGACAAGAGCGGCCTGCACCTAGGGAAGCC GGGCCACAGGTCTTATGCGCTGTCCCCCCACACGGTCATTCAGACCACACACAGTGCATCAGAGCCTCTC CCGGTGGGCCTACCAGCCACGGCCTTCTACGCTGGCACTCAACCTCCTGTCATCGGCTACCTGAGCGGCC AGCAGCAAGCAATCACCTATGCTGGTGGTCTGCCGCAGCACCTGGTGATCCCAGGTAACCAGCCCCTGCT CATCCCGGTGGGCAGCCCTGACATGGACACGCCTGGGGCAGCCTCGGCCATCGTGACGTCATCACCCCAG TTTGCTGCAGTACCTCACACGTTTGTCACCACCGCCCTGCCCAAGAGCGAGAACTTCAACCCAGAGGCTC TGGTCACCCAGGCTGCCTACCCAGCCATGGTGCAGGCCCAGATCCACCTGCCGGTGGTGCAGTCCGTGGC GTCCCCCACCACGGCGTCTCCCACGCTGCCGCCATATTTCATGAAAGGCTCCATCATCCAGCTGGCCAAC GGGGAGCTGAAGAAGGTGGAGGACCTGAAGACGGAGGATTTCATCCAGAGTGCAGAGATTAGCAATGACC TCAAGATCGACTCCAGTACTGTGGAGAGAATCGAGGAGAGCCACAGCCCCGGGGTGGCCGTGATACAGTT TGCTGTTGGTGAACACCGAGCCCAGGTCAGTGTCGAAGTCTTGGTAGAGTATCCTTTTTTTGTATTTGGA CAGGGCTGGTCATCCTGCTGTCCTGAGCGGACCAGCCAGCTCTTTGATCTGCCGTGTTCCAAACTCTCTG TTGGGGACGTCTGCATCTCGCTCACCCTCAAGAACCTGAAGAATGGCTCTGTTAAAAAGGGCCAGCCTGT GGACCCTGCCAGCGTCCTGCTGAAGCAGGCAAAGACCGACAGCCTGGCTGGCAGCAGACACAGATACGCG GAGCAGGAAAACGGAATCAACCAGGGAAGCGCCCAGGTGCTCTCTGAGAATGGCGAACTGAAGTTTCCAG AAAAAATAGGATTGCCTGCAGCACCCTTCCTCAGCAAAATAGAACCGAGCAAACCCACAGCCACGAGGAA GAGGAGGTGGTCGGCGCCGGAGACCCGTAAACTGGAGAAGTCGGAGGACGAGCCACCTTTGACTCTTCCC AAGCCTTCGCTCATTCCTCAGGAGGTTAAGATCTGCATCGAAGGCCGATCTAACGTGGGCAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199304
Insert Size	2376 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199304.1, NP_001186233.1
RefSeq Size	10474 bp
RefSeq ORF	2376 bp
Locus ID	20238
UniProt ID	P54254
Gene Summary	Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression (By similarity). May be involved in RNA metabolism (By similarity). In concert with CIC and ATXN1L, involved in brain development (PubMed:28288114).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 2 and 3 both encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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