H6pd (NM_001291004) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI785303; G6pd1; Gpd-1; Gpd1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228938 representing NM_001291004 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCTAGCGGCAATGTGCTTGGCCCTTCTGGGCTGCCTACAGGCGCAGGAACTCAAGGGTCATGTTT CCATAATCCTGCTGGGAGCAACCGGGGACCTAGCTAAAAAGTACCTGTGGCAGGGACTGTTTCAGCTATA CCTGGATGAGGCTGGGAAGGGCCACAGTTTCAGCTTCCACGGTGCTGCCCTGACAGCCCCCCAGCAAGGC CAGAAGCTCATGGACAAGGTCTTGGAATCCCTCTCCTGTCCCAAGGACTTGGTACCCAGTCGCTGTGATG AACTCAAGGGTCAGTTTCTACAGCTGAGCCAATACCGCCAGCTGAAGACAGTGGAGGACTATCAGACCCT CAACAAGGATATTGAGACCCAGGTCCAGCAGGATGGGCTCTGGGAGGCAGGCAGGATCTTCTACTTCAGT GTGCCGCCCTTTGCCTACGCAGACATTGCCCGTAACATCAACAGCAGCTGCCGACCACACCCAGGTGCCT GGCTACGGGTTGTTTTTGAAAAACCCTTTGGCCATGACCACCTCTCAGCCCAGCAGCTGGCTTCAGAACT TGGGAGCTTTTTCCAGGAGGAGGAGATGTACCGTGTGGATCATTACCTCGGCAAGCAGGCGGTGGCTCAG ATCCTGCCCTTCCGAGATCAGAACCGCAAGGCCTTGGATGGCCTCTGGAACAGGCACCATGTGGAGCGGG TGGAGATCATCTTGAAGGAGACCATAGATGCGGAAGGTCGGGCCAGTTTCTATGAGGAGTATGGCGTCAT CCGTGACACGCTGCAGAACCACCTGACTGAGATCCTCACGCTGGTGGCCATGGAACTGCCTCTCAACATC AGCAGCTCAGCCGCCGTGCTCCAGCATAAGCTCTGGGCCTTCCAGGCCCTGCGGGGGCTGCAGAAGAGCA GTGCCATCCTGGGCCAGTACCAGGCCTACAGCGGACAGGTGCGCCGAGAGCTGCAGAAGCCAGACGGCTT CCAGAGCCTGACACCAACCTTCGCAGGTGTCCTTGTCCACATCGACAACCTGCGCTGGGAGGGTGTCCCT TTCATCCTGATGTCTGGCAAGGCCTTGGATGAGAGAGTGGGCTATGTTCGGATTGTGTTTAAGAATCGGG CGTACTGCACCCAGAGCGAGAGACACTGGGTGCCGGAGCAGAGCCGGTGCCTGCCCCAGCAGATTATCTT CTACATTGGACACGGTGAGCTGGGCCACCCTGCCATTCTGGTCAGCCGGAACCTGTTCAAACCCTCACTA CCCACTCAGAAGTGGAAGGAGGTGCAGGACCAGCCTGGGCTCCGCCTGTTCGGCCGTCCTCTGTCTGATT ACTACGCCTACAGGCCTGTGCGGGAGCAAGACGCCTACTCCACCCTCCTGTCGCACATCTTCCACTGCCG AAAGGAGTCCTTCATCACCACAGAGAACCTCTTGGCCTCCTGGGTCTTCTGGACCCCGTTACTGGACAGC CTGGCCTTTGAAGTTCCACGCCCATACCCAGGGGGAGCCGAGAATGGCCAGCTGCTGGACTTTGAGTTTA GCGGTGGTCAGCTGACCTTCTCCCAGCAGCAGCTGGAGGTGCTGATACCAGATCTAGGCTCAGTCCCAAA GCCCAGTGACTTCCAGGTCCTCGGGGCCCGGTATAGACAGAGCCCACTGATTACCGCCTGGCCAGAGGAG CTGATCTCTAAGCTGGCCAGTGACATTGAAGCCGCCGCGGTGCAGGCAGTACGGCACTTCGGCAAGTTCC ACCTGGCACTGTCAGGTGGTTCGAGCCCCATAGCTCTCTTCCAGCAGCTGGCCACAGGACATTACAGCTT CCCCTGGGCCCACACACACCTCTGGCTGGTTGATGAGCGTTGTGTCCCTCTCTCAGATCCAGACTCCAAC TTCCAGGGCCTGCAGGCTCACCTGCTACAGCACGTCAGGGTACCCTACTACAACATCCACCCGATGCCAG TGCACCTGCACCAGCGGCTCTGTGCCGAGGAGGACCAGGGAGCTCAGACCTATGCCAGTGAGATCTCAGC CCTGGTGGCCAACAGCAGCTTCGACCTGGTGCTCCTGGGCATGGGCACCGACGGGCACACAGCTTCTCTG TTCCCACAGTCCCCCACAGGCCTGGATGGTGATCAGCTGGTGGTGCTGACGGAGAGCCCGTTCAGGCCAC ACCAACGCATGAGCCTCAGCCTGCCCCTCATCAACCGAGCCAAGAAGGTAGCTGTCCTGGTCATGGGCAG GACAAAACGTGAGATCACAACGTTGGTGAGCCGCGTGGGCCATGAACCCAAGAAGTGGCCCATCTCAGGT GTGGTGCCTCTTTCTGGGCAGCTGGTTTGGTACATGGATTACGAGGCCTTTCTGGGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291004
Insert Size	2370 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291004.1, NP_001277933.1
RefSeq Size	4678 bp
RefSeq ORF	2370 bp
Locus ID	100198
UniProt ID	Q8CFX1
Gene Summary	Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus, compared to isoform 1.

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