Plcd1 (NM_001293648) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW212592; C79986
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228924 representing NM_001293648 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTGTCTAGGGGTCCGGAGTTGCAGACGGGGCAGCGTGAACCGCTCCCGGGAGCTCTACCTGCAGG AGCAGAGTCGCAAAGTAGCGGCGCTCAATGGACAGAGACTGGGGCTGCAGGATGACCCGGACCTTCAGGC CCTCCTGAAGGGCAGCCAGCTTCTGAAGGTGAAGTCCAGCTCGTGGCGTAGAGAACGCTTCTACAAGCTA CAGGAGGACTGCAAGACCATCTGGCAGGAATCTCGCAAGGTCATGAGGTCCCCGGAGTCGCAGCTGTTCT CCATCGAGGACATTCAGGAGGTGCGGATGGGACACCGCACAGAAGGCCTGGAGAAATTTGCCAGAGACAT ACCCGAGGACCGATGCTTCTCCATCGTCTTCAAGGACCAGCGCAATACCCTAGACCTCATCGCCCCGTCT CCAGCTGACGTCCAGCATTGGGTGCAGGGCCTGCGCAAGATCATCGACCGCTCGGGCTCCATGGACCAGC GGCAGAAGCTGCAGCACTGGATTCACTCCTGCTTGCGAAAGGCTGATAAAAACAAGGATAACAAGATGAA CTTCAAGGAGGTGAAGGACTTCCTGAAGGAGCTCAACGTACAGGTGGATGACAGCTACGCCAGGAAGATC TTCAGGGAATGTGACCACTCCCAGACAGACTCGCTGGAGGATGAGGAGATTGAGACCTTCTACAGGATGC TGACCCAGCGGGCAGAGATCGACCGTGCCTTTGCAGAGGCCGCAGGTTCTGCGGAGACCCTGTCGGTGGA GAAGCTAGTGACGTTCCTGCAGCATCAGCAGCGGGAGGAGGAGGCGGGGCCAGCCTTGGCCCTCTCTCTC ATTGAGCGCTACGAGCCCAGTGAGACTGCCAAAGCCCAGCGGCAGATGACCAAGGATGGCTTCCTCATGT ACCTGCTGTCTGCTGACGGTAACGCCTTCAGCCTGGCGCACCGCCGTGTCTACCAGGACATGAACCAGCC GCTCAGCCACTACTTAGTGTCTTCTTCCCACAACACCTACCTACTGGAGGACCAGCTCACAGGGCCCAGC AGCACGGAGGCCTACATCCGGGCTCTGTGCAAAGGCTGCCGGTGTCTGGAGCTGGACTGCTGGGACGGCC CCAACCAGGAACCCATCATCTACCATGGCTACACTTTTACCTCTAAGATACTTTTCTGCGACGTGCTCAG GGCCATCCGGGACTACGCCTTCAAGGCATCCCCCTACCCAGTTATCCTGTCCCTGGAGAACCACTGCAGC CTGGAGCAACAACGAGTCATGGCTCATCACCTGAGGGCCATCCTGGGGCCCATGCTGTTGGACCAGCCGC TGGATGGGGTTACCACGAGCCTGCCTTCGCCTGAGCAACTGAAGGAAAAGATTCTGTTGAAAGGGAAGAA ACTGGGCGGGCTGCTACCTGCTGGAGGGGAAAACGGGCCTGAAGCCACCGATGTGTCTGACGAAGATGAG GCTGCTGAAATGGAAGATGAGGCCGTGCGCAGCCAAGTGCAACACAAGCCCAAGGAGGATAAGCTAAAGC TGGTGCCGGAGCTCTCCGACATGGTCATTTACTGCAAGAGTGTCCACTTTGGTGGCTTCTCCAGTCCTAG CACCTCTGGGCAGGCATTCTATGAGATGGCTTCCTTCTCCGAGAGCCGTGCCCTGCGGCTGCTGCAAGAA TCAGGAAATAGTTTTGTCCGCCATAACGTGGGCCATCTGAGCAGGATCTACCCAGCTGGGTGGAGGACAG ATTCCTCCAACTACAGTCCCGTGGAGATGTGGAATGGAGGCTGCCAGATTGTGGCTCTGAACTTCCAAAC CCCTGGGCCAGAAATGGATGTTTACCTTGGCTGCTTCCAGGACAATGGAGGTTGTGGGTATGTGCTGAAA CCGGCCTTCCTGAGAGACCCTGACACCACCTTCAACTCACGGGCCCTGACTCAGGGGCCCTGGTGGGCCC CGAAGAAGCTCCGAGTCTGGATCATCTCTGGACAGCAGCTGCCGAAGGTCAATAAGAATAAGAATTCTAT CGTGGACCCCAAGGTGATAGTGGAGATCCACGGTGTGGGCCAGGATGTGGCCAGCCGTCAGACAGCAGTT ATTACCAATAATGGTTTCAACCCACGGTGGGACACGGAGTTTGAGTTTGTGGTAGCAGTGCCTGACCTTG CCCTTGTACGTTTCATGGTAGAGGATTATGACTCCTCCTCTAAGAACGACTTTATCGGCCAGAGTACTAT CCCCTGGAACAGCCTCAAGCAGGGGTACCGCCATGTCCACCTCTTGTCTAAGAATGGAGACCTGCATCCA TCAGCCACTCTCTTTGTGAAGATCTCCATCCAGGACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001293648
Insert Size	2349 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001293648.1, NP_001280577.1
RefSeq Size	2706 bp
RefSeq ORF	2349 bp
Locus ID	18799
UniProt ID	Q8R3B1
Gene Summary	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential for trophoblast and placental development.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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