Rabep1 (NM_001291143) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Rab5ep; rabaptin-5; rabaptin-5alpha
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228918 representing NM_001291143 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAACATTAAGGCAATTGCCACAGTCTCTGAGAATACTAAGCAAGAAGCTATAGATGAAGTTAAAA GGCAATGGAGAGAAGAAGTTGCTTCACTTCAGGCTATAATGAAAGAGACAGTCCGTGACTATGAGCATCA GTTTCACCTGAGGCTGGAGCAGGAGCGAGCACAGTGGGCACAGTATCGAGAATCTGCAGAGCGGGAAATA GCTGACTTAAGAAGAAGGTTGTCTGAAGGTCAAGAGGAAGAAAATTTAGAAAATGAAATGAAAAAGGCCC AAGAGGATGCTGAAAAGCTTCGTTCTGTCGTGATGCCCATGGAGAAGGAAATTGCAGCTCTGAAAGATAA ACTGACAGAAGCTGAAGACAAGATTAAAGAGCTGGAGGCCTCAAAGGTTAAGGAGCTGAATCATTATCTG GAGGCCGAGAAGTCTTGCAGGACTGACCTGGAGATGTACGTAGCTGTTTTGAACACTCAGAAATCTGTGC TACAGGAAGATGCTGAGAAATTGAGGAAAGAACTGCATGAAGTTTGCCATCTCTTGGAACAAGAACGACA ACAACACAACCAGTTAAAGCATACATGGCAGAAGGCCAACGACCAGTTTCTGGAATCTCAGCGTTTGCTG ATGAGAGACATGCAGAGAATGGAGATTGTGTTAACTTCGGAACAGCTCCGACAAGTTGAAGAACTAAAGA AGAAAGACCAGGAGGAGGATGAACAACAAAGGGTCAATAAGAGAAAAGATAACAAGAAAACAGATACCGA AGAAGAAGTAAAAATACCAGTAGTGTGTGCTTTAACTCAAGAAGAATCTTCAACCCCATTATCAAATGAA GAGGAGCATTTAGACAGCACCCATGGGTCAGTCCATTCCTTGGATGCAGACTTGATGTTACCATCTGGAG ATCCATTTAGTAAATCGGACAATGACATGTTTAAAGATGGACTCAGGAGAGCTCAGTCTACAGACAGCTT GGGAACCTCAAGCTCATTGCAATCCAAAGCTTTAGGCTATAACTACAAAGCGAAATCTGCTGGAAATTTG GATGAGTCGGATTTTGGACCACTGGTTGGAGCAGATTCTGTGTCTGAGAACTTTGATACTGTGTCCCTTG GGTCACTGCAGATGCCAAGTGGATTTATGTTAACCAAAGATCAAGAAAGAGCAATTAAGGCAATGACACC TGAGCAGGAGGAGACAGCATCCCTCCTCTCCAGTGTCACCCAGGGTATGGAGAGTGCATATGTATCTCCC AGTGGTTACCGCTTAGTGAGTGAGACGGAATGGAATCTCCTGCAGAAAGAGGTACATAATGCTGGAAATA AGCTTGGTAGACGTTGTGATATGTGTTCCAATTATGAAAAACAGTTACAAGGAATTCAGATTCAGGAAGC TGAAACAAGAGACCAGGTGAAAAAACTGCAGTTAATGCTCAGGCAAGCTAATGACCAGCTTGAGAAGACG ATGAAAGAGAAGCAGGAGCTGGAGGACTTCCTCAAGCAGAGTGCGGAGGACTCAAGCCACCAGATATCTG CACTTGTTTTAAGAGCCCAAGCCTCTGAGGTCTTACTTGAAGAGTTACAACAGAGCTTTTCCCAAGCAAA GAGGGACGTTCAGGAGCAGATGGCGGTGCTGATGCAGTCACGTGAACAGGTTTCAGAAGAGTTGGTGAGG TTACAGAAAGACAACGACAGCCTCCAGGGGAAGCACAGCTTGCATGTGTCATTACAGCTAGCAGAAGACT TCATTCTTCCAGACACTGTGGAGGTGCTCCGGGAACTGGTTTTAAAATATCGTGAGAACATTGTTCATGT GCGCACAGCAGCAGACCACATGGAAGAGAAGCTGAAGGCGGAAATACTTTTCCTAAAAGAGCAAATTCAA GCGGAACAGTGTTTAAAAGAAAACCTGGAAGAGACACTACAGCTGGAAATAGAAAACTGCAAGGAGGAAA TAGCTTCCATTTCTAGTCTAAAAGCTGAATTAGAAAGAATAAAGGTTGAAAAGGGACAGTTGGAATCTAC ATTAAGAGAGAAGTCGCAACAACTTGAGAGTCTCCAAGAAATGAAAGTTAATTTAGAGGAACAGTTAAAG AAAGAAACTGCTGCTAAGGCTACTGTTGAACAGCTCATGTTTGAAGAGAAGAACAAAGCTCAGAGGTTGC AGACAGAATTAGATGTCAGTGAACAAGTACAGAGAGATTTTGTAAAACTTTCTCAGACTCTTCAGGTGCA GTTGGAGAGGATCCGGCAAGCAGACTCCTTGGAGAGGATCCGGGCTATTCTGAATGATACCAAACTGACA GACATTAACCAGCTCCCTGAGACATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291143
Insert Size	2337 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291143.1, NP_001278072.1
RefSeq Size	5511 bp
RefSeq ORF	2337 bp
Locus ID	54189
UniProt ID	O35551
Gene Summary	Rab effector protein acting as linker between gamma-adaptin, RAB4A and RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Involved in KCNH1 channels trafficking to and from the cell membrane. Stimulates RABGEF1 mediated nucleotide exchange on RAB5A. Mediates the traffic of PKD1:PKD2 complex from the endoplasmic reticulum through the Golgi to the cilium (PubMed:25405894).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in its 5' UTR and uses a downstream start codon, compared to variant 1. The encoded isoform (4) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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