Eps15l1 (NM_001289859) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9830147J04Rik; AI593686; Eps15-rs; Eps15R
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228880 representing NM_001289859 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCCGCTTGTACCCCTCTCGCAGCAGATTCCCGGTGGAAATCCGTTATACGAGTCTTATTACA AGCAGGTGGACCCTGCCTACACAGGGAGAGTTGGGGCAAGCGAGGCTGCGCTGTTTCTGAAGAAATCGGG GCTCTCAGACATCATCCTGGGGAAGATATGGGACTTGGCAGACCCCGAAGGTAAAGGGTTCTTGGACAAA CAGGGTTTCTATGTTGCACTGCGACTGGTGGCCTGTGCACAGAGTGGCCATGAAGTCACCTTGAGCAGTC TGAGCCTCACCATGCCACCACCAAAATTTCATGATACCAGCAGCCCACTGATGGCCACACAGTCATCTGC AGAGACCCACTGGGCTGTGCGGGTGGAGGAGAAGGCAAAGTTTGATGGCATCTTTGAAAGCCTTCTACCA GTCAATGGGCTACTCTCTGGAGACAAGGTCAAGCCGGTCCTCATGAACTCGAAACTACCTCTGGATGTGC TGGGCAGGGTCTGGGACCTCAGTGACATAGACAAGGATGGACATTTGGACCGAGATGAGTTTGCTGTGGC TATGCACTTGGTGTACCGCGCCCTGGAGAAGGAACCAGTGCCCTCCATCCTGCCCCCGCCCCTCATCCCA CCCTCTAAGAGGAAGAAGACTGTGTTTGCAGGAGCTGTGCCTGTCCTGCCTGCTAGTCCCCCACCCAAAG ACAGCCTCCGCTCCACACCATCCCACGGCAGCGTAAGCAGCCTCAACAGCACAGGCAGCCTGTCCCCGAA GCACAGCGTCAAGCAGCCACCAGTGGCCTGGGTTGTGCCTGTGGCAGATAAGATGCGCTTTGATGAGATC TTTTTGAAGACAGACCTGGACCTTGATGGCTATGTGAGTGGCCAGGAAGTTAAGGAGATCTTCATGCACT CAGGCCTCACTCAGAACCTTCTGGCACACATCTGGGCCCTGGCTGATACAAGACAAACGGGCAAGTTAAG CAAAGAGCAGTTCGCACTAGCTATGTATTTCATTCAGCAGAAGGTCAGTAAAGGCATCGACCCTCCTCAA GTCCTCTCGCCTGACATGGTGCCACCCTCTGAGAGAGGCACTCCCATTCCAGACAGTTCAAGTACTCTGG CATCAGGAGAGTTCACTGGTGTTAAAGAGCTGGATGACATTAGCCAGGAGATCGCACAGTTGCAGAGAGA GAAATACTCATTGGAACAAGACATCAGAGAAAAGGAAGAGGCAATCAGACAGAAAACCAGCGAAGTACAG GAATTACAAAATGACCTAGACCGGGAAACTAGTAGTCTGCAGGAGCTTGAGGCTCAGAAACAGGATGCCC AGGACCGCCTGGATGAGATGGACCAGCAGAAGGCTAAGCTGCGGGACATGCTGAGTGACGTGCGGCAGAA ATGCCAGGATGAGACCCAGACAATCTCATCATTGAAAACCCAGATTCAGTCTCAGGAGTCAGACTTGAAG TCCCAGGAGGATGACCTGAACAGGGCCAAGTCAGAGCTGAATCGGCTGCAGCAAGAGGAGACACAGCTGG AGCAGAGCATCCAGGCTGGACGTGCACAGCTGGAGACCATCCTCAGGTCCCTGAAGTGCACACAGGATGA CATCAACCAGGCAAGAAGCAAGCTGTCCCAGCTACAGGAGAGCCACCTAGAAGCTCACCGGAGCCTGGAA CAGTATGACCAGGATGATCCTTTCAAAAACAAAGCCTTATTGTTTAGCAACAACTCCCAGGAACTTCATC CAGACCCCTTCCAGGCTGAGGACCCCTTCAAGTCCGACCCATTTAAAGGAGCTGACCCCTTCAAAGGTGA TCCTTTCCAAAGTGATCCCTTCTCAGAGCAGCAGACAGCAGCTACAGACCCATTTGGAGGGGATCCTTTC AAAGAAAGTGACCCATTCCACAGCTCAAGCAGTGATGACTTCTTCAAGAAACAGACAAAGAATGACCCAT TTACCTCAGACCCCTTCACAAAGAACCCTTCCTTACCTTCAAAGCTCGACCCATTTGAGTCCAGTGATCC TTTTTCATCCTCCAGCATCTCCTCGAAAGGTTCAGATCCCTTTGGAACCCTAGACCCCTTTGGAAGCAGC TCCTTCAGCAGTGCCGAGGGCTTCGCTGACTTCAGTCAGATGTCCAAGTTTGGCAATGAGGAGCAGCAAT TGGCTTGGGCCAAGCGGGAGAGTGAAAAAGCGGAGCAAGAGCGGCTGGCGAGGCTGCGGCGACAGGAACA GGAGGACCTGGAACTGGCCATCGCCCTCAGCAAGGCTGATATGCCAGCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289859
Insert Size	2292 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289859.1, NP_001276788.1
RefSeq Size	2727 bp
RefSeq ORF	2292 bp
Locus ID	13859
UniProt ID	Q60902
Gene Summary	Seems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate in-frame splice site and lacks two consecutive exons in the 3' coding region, compared to variant 1. It encodes isoform c, which is shorter than isoform a.

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