Kcnc3 (NM_001290682) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Kcr2-3; KShIIID; Kv3.3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228877 representing NM_001290682 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAGTTCAGTGTGCGTCTGGTCGTTCCGCGGGCGCCAGGGGACCGGCAAGCAGCAGCCTCAGCCGG TGCCAACGCCGCAGCCGCCTGAGTCCTCACCGCCGCCTCTGCCGCCGCCGCAGCAGCAGCAGTGCTCTCA GCCCGGCACTGCCGCCTCCCCGGCGGGTGCCCCGCTTTCCTGCGGGCCTGGGGGCCGGCGTGCCGAGCCA TGCCCCGGGCTGCCGGCGGTGGCCATGGGGCGGCACGGCGGCGGCGGCGGCGACAGCGGTAAGATCGTGA TCAACGTGGGCGGCGTGCGCCATGAGACGTACCGCTCCACGTTGCGCACCCTGCCAGGGACCAGACTGGC CGGGCTGACCGAGCCCGAGGCGGCCGCGCGCTTTGACTACGACCCGGGCACGGACGAGTTCTTCTTCGAC CGTCACCCGGGCGTCTTCGCCTACGTGCTCAACTACTACCGCACCGGCAAACTGCACTGCCCGGCCGACG TGTGCGGGCCGCTCTTCGAGGAGGAGCTGGGCTTCTGGGGCATAGACGAGACGGACGTGGAGGCCTGCTG CTGGATGACCTATCGCCAGCACCGTGACGCTGAGGAGGCACTGGACTCTTTCGAGGCCCCGGACTCCTCG GCCAACGCCAACGCCAACGCCGGAGGCGCGCACGATGCGGGACTGGACGACGAGGCGGGCGCAGGAGGCG GCGGCCTGGACGGGGCAGGCGGCGAGCTCAAGCGTCTGTGTTTTCAGGACGCGGGCGGAGGTGCCGGAGG ACCTGCCGGGGGCGCGGGCGGCGCGGGCGGCACCTGGTGGCGGCGCTGGCAGCCCCGTGTGTGGGCGCTT TTTGAGGACCCCTACTCGTCGCGGGCTGCCAGGTATGTGGCCTTCGCCTCCCTATTCTTTATCCTCATCT CCATCACCACCTTCTGCCTGGAGACACACGAGGGCTTCATCCACATCAGCAACAAGACGGTGACGCAGGC CTCCCCAATCCCTGGGGCTCCCCCGGAGAATATCACCAACGTGGAGGTGGAGACGGAACCCTTCTTGACC TACGTGGAAGGCGTGTGTGTGGTCTGGTTCACCTTTGAGTTTCTCATGCGGGTCACCTTCTGCCCAGATA AGGTGGAGTTTCTCAAAAGCAGCCTGAACATCATCGACTGCGTGGCCATCTTGCCCTTCTACTTGGAAGT GGGCCTGTCAGGTCTCAGCTCCAAAGCTGCCAAGGACGTGCTGGGCTTCCTGCGTGTCGTCCGCTTCGTG CGCATCCTTCGTATCTTCAAGCTGACCCGTCACTTCGTGGGGCTGCGTGTGCTGGGCCACACGCTCCGGG CCAGCACCAACGAATTCTTGTTACTCATCATCTTCCTGGCTCTGGGGGTCCTCATCTTTGCCACCATGAT CTACTATGCCGAACGCATCGGGGCTGATCCTGACGACATCCTGGGCTCCAACCACACCTACTTCAAGAAC ATCCCCATCGGCTTCTGGTGGGCTGTGGTCACCATGACGACACTGGGCTATGGAGACATGTATCCCAAGA CGTGGTCTGGGATGCTGGTTGGGGCACTGTGTGCCCTGGCTGGTGTGCTGACCATTGCCATGCCCGTGCC TGTCATTGTCAACAACTTCGGCATGTACTATTCACTGGCTATGGCCAAGCAGAAATTGCCAAAGAAGAAA AACAAACATATTCCCAGGCCCCCGCAGCCTGGCTCACCCAACTACTGCAAGCCTGACCCCCCGCCTCCAC CCCCACCACACCCCCACCACGGCAGCGGTGGCATAAGCCCACCGCCGCCCATCACCCCTCCTTCCATGGG GGTGAATGTGGCCGGGGCCTACCCACCTGGACCCCACACACACCCCGGGCTGCTCAGGGGTGGTGCTGGG GGCCTGGGAATCATGGGATTGCCTCCTCTGCCAGCCCCTGGTGAGCCCTGCCCATTGGCTCAAGAAGAGG TGATTGAAACCAACAGGGCAGACCCCCGTCCCAATGGAGACCCTGCAGCAGCCGCACTGGCCCATGAGGA CTGCCCTGCCATCGACCAGCCAGCCATGTCTCCAGAAGACAAGAGCCCAATCACTCCCGGAAGCCGGGGT CGCTACAGCCGGGACCGAGCTTGCTTCCTTGTCACAGACTATGCCCCTTCCCCTGATGGCTCCATCCGAA AAGCCACTGGTGCTCCCCCACTGCCCCCCCCATGCTGGCATAAGCCAGGCCCCCCAAGCTTCTTGCCCGA CCTCAACGCCAACGCAGCAGCCTGGATATCCCCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290682
Insert Size	2277 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290682.1, NP_001277611.1
RefSeq Size	5184 bp
RefSeq ORF	2277 bp
Locus ID	16504
Gene Summary	Voltage-gated potassium channel that plays an important role in the rapid repolarization of fast-firing brain neurons. The channel opens in response to the voltage difference across the membrane, forming a potassium-selective channel through which potassium ions pass in accordance with their electrochemical gradient. The channel displays rapid activation and inactivation kinetics (PubMed:18539595, PubMed:26997484, PubMed:24218544). It plays a role in the regulation of the frequency, shape and duration of action potentials in Purkinje cells (PubMed:15217387, PubMed:18448641, PubMed:24218544). Required for normal survival of cerebellar neurons, probably via its role in regulating the duration and frequency of action potentials that in turn regulate the activity of voltage-gated Ca(2+) channels and cellular Ca(2+) homeostasis (PubMed:24218544). Required for normal motor function (PubMed:16923152, PubMed:18448641). Plays a role in the reorganization of the cortical actin cytoskeleton and the formation of actin veil structures in neuronal growth cones via its interaction with HAX1 and the Arp2/3 complex (PubMed:26997484).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains an alternate exon compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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