Sun1 (NM_001256118) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4632417G13Rik; 5730434D03Rik; mKIAA0810; Unc84a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228875 representing NM_001256118 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTTTTCTCGGCTGCACACGTACACCCCACCCCAGTGTGTGCCGGAGAACACTGGCTACACTTACG CACTCAGTTCTAGTTACTCGTCGGATGCTCTGGATTTTGAAACTGAGCACAAGTTGGAACCTGTATTTGA CTCTCCAAGGATGTCCCGCCGCAGCTTGCGTCTGGTCACAACAGCTTCGTACAGCAGTGGGGACAGCCAG GCTATTGATTCGCACATTAGCACCAGCAGGGCCACCCCCGCCAAGGGGAGAGAAACCAGGACAGTCAAAC AGAGAAGAAGTGCAAGCAAGCCAGCTTTTAGTATCAACCACCTGTCAGGGAAGGGCTTGTCCTCGAGCAC AAGCCATGACAGCTCTTGCAGCCTGCGGAGTGCCACGGTGCTGCGGCACCCTGTGCTAGATGAGTCCCTG ATTCGTGAGCAGACCAAAGTGGACCACTTCTGGGGTCTCGATGATGATGGTGACCTTAAAGGTGGAAATA AAGCTGCCACTCAGGGAAATGGTGAACTGGCAGCAGAGGTGGCGAGCAGCAATGGATACACTTGCCGTGA CTGCAGGATGCTCTCAGCGCGCACTGACGCACTCACAGCCCACTCTGCCATCCACGGGACCACCTCCAGG GTGTACTCCAGAGACAGGACTCTCAAACCACGGAAGGCAGCCTCGGGAACCTTCTGGTGGCTAGGGAGCG GCTGGTACCAATTTGTTACTTTGATTTCTTGGCTGAATGTCTTTCTTCTTACCAGGTGCCTTCGAAATAT TTGCAAGGTTTTTGTCTTGCTCCTCCCACTCCTACTTTTACTAGGTGCTGGTGTCTCCCTGTGGGGCCAG GGAAACTTCTTCTCACTCCTACCAGTGCTGAACTGGACGGCCATGCAGCCAACACAGAGGGTGGACGATT CCAAGGGCATGCATAGACCTGGCCCTCTTCCCCCGAGCCCACCTCCAAAGGTTGATCACAAGGCTTCCCA GTGGCCTCAGGAGAGTGACATGGGGCAGAAGGTAGCTTCTTTGAGTGCGCAGTGCCACAACCATGATGAG AGACTTGCAGAGCTGACAGTCCTGCTTCAGAAACTACAGATACGGGTAGACCAAGTGGATGACGGCAGGG AAGGGCTGTCACTGTGGGTCAAGAATGTGGTTGGACAGCACCTGCAGGAGATGGGCACCATAGAACCACC TGATGCTAAGACTGACTTCATGACTTTCCACCATGACCATGAAGTGCGTCTCTCCAACTTGGAAGATGTT CTTAGAAAACTGACAGAAAAATCTGAGGCTATCCAGAAGGAGCTGGAAGAAACCAAGCTGAAAGCAGGCA GCAGGGATGAAGAGCAGCCCCTCCTTGACCGTGTGCAGCACCTAGAACTGGAACTGAACCTGTTGAAGTC ACAGCTGTCAGACTGGCAGCATCTGAAGACCAGCTGTGAGCAGGCTGGGGCCCGCATCCAGGAGACTGTG CAGCTCATGTTCTCTGAGGATCAGCAGGGCGGTTCCCTCGAGTGGCTATTAGAGAAGCTTTCTTCTCGGT TCGTGAGCAAGGATGAGCTGCAGGTGCTCTTACATGACCTTGAGCTGAAACTGCTGCAGAATATCACACA CCACATCACCGTGACAGGACAGGCCCCGACATCCGAGGCTATTGTGTCTGCCGTGAATCAGGCAGGGATT TCAGGAATCACAGAAGCGCAAGCACATATCATTGTGAACAATGCTCTGAAGCTGTACTCCCAAGACAAGA CGGGGATGGTGGACTTTGCTCTGGAGTCTGGAGGTGGCAGCATCCTAAGCACTCGGTGCTCTGAGACCTA TGAGACCAAGACGGCACTGCTGAGCCTGTTTGGGGTCCCACTGTGGTACTTCTCACAGTCACCTCGAGTG GTGATCCAGCCCGACATCTACCCAGGGAATTGCTGGGCGTTCAAAGGTTCCCAGGGGTACCTGGTGGTGC GGTTGTCCATGAAGATCTACCCAACCACATTCACCATGGAACACATTCCAAAGACACTATCACCCACTGG TAACATCTCCAGTGCCCCCAAAGACTTTGCAGTCTATGGACTGGAAACGGAGTATCAAGAAGAGGGGCAG CCTCTGGGACGGTTCACCTATGACCAGGAAGGAGACTCACTCCAGATGTTCCACACACTGGAAAGACCTG ACCAAGCCTTCCAGATAGTAGAGCTCCGGGTCCTGTCCAACTGGGGCCACCCTGAGTACACTTGCCTCTA CCGGTTCCGAGTCCACGGAGAGCCCATCCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256118
Insert Size	2274 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256118.1, NP_001243047.1
RefSeq Size	3602 bp
RefSeq ORF	2274 bp
Locus ID	77053
UniProt ID	Q9D666
Gene Summary	As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton (PubMed:20711465, PubMed:16380439, PubMed:24062341, PubMed:25892231, PubMed:26842404). The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning (PubMed:19874786). Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration (PubMed:19874786). Involved in telomere attachment to nuclear envelope in the prophase of meiosis implicating a SUN1/2:KASH5 LINC complex in which SUN1 and SUN2 seem to act at least partial redundantly (PubMed:17543860, PubMed:19211677, PubMed:19509342, PubMed:24062341, PubMed:25892231, PubMed:26842404). Required for gametogenesis and involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis (PubMed:17543860). Helps to define the distribution of nuclear pore complexes (NPCs) (PubMed:17724119). Required for efficient localization of SYNE4 in the nuclear envelope (PubMed:23348741). May be involved in nuclear remodeling during sperm head formation in spermatogenenis (PubMed:20711465). May play a role in DNA repair by suppressing non-homologous end joining repair to facilitate the repair of DNA cross-links (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) lacks four consecutive exons in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (5) lacks an internal segment, compared to isoform 1.

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