Tle4 (NM_001302950) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5730411M05Rik; AA792082; Bce-1; Bce1; ESTM13; ESTM14; grg-4; Grg4; X83332; X83333
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228847 representing NM_001302950 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCGCGACCTGAGCAAGATGTACCCGCAGACGCGCCACCCGGCACCGCATCAGCCTGCTCAACCCT TCAAATTTACAATTTCAGAATCCTGTGATCGGATTAAGGAAGAGTTTCAGTTTTTACAGGCTCAATACCA CAGTCTGAAGCTGGAATGTGAGAAGCTCGCCAGCGAGAAGACAGAGATGCAGCGGCATTATGTCATGTAT TATGAAATGTCCTATGGGTTGAACATAGAAATGCACAAGCAGGCAGAGATTGTTAAACGACTAAATGCTA TCTGTGCACAGGTCATTCCTTTCCTGTCCCAAGAGCAGCAACAACTCCAAGCTCAGCATTTATCACATGG ACATGGTCTGCCTGTGCCTCTGACACCACACCCTTCAGGGCTTCAGCCCCCAGCCATCCCACCTATCGGT AGCAGTGCAGGACTTCTGGCCCTCTCCAGTGCACTAGGAGGTCAGTCTCACCTCCCAATTAAGGATGAGA AGAAGCACCATGACAATGATCACCAAAGAGACAGAGACTCCATCAAGAGCTCTTCCGTATCCCCATCAGC CAGTTTCCGGGGTTCTGAGAAACATAGGAACTCTACAGACTATTCCTCAGAGAGCAAAAAGCAGAAAACA GAAGAAAAAGAAATTGCAGCTCGCTATGACAGTGATGGTGAGAAGAGTGATGACAACTTGGTAGTTGATG TGTCCAATGAGGATCCATCTTCCCCCCGAGGAAGCCCAGCACATTCCCCAAGGGAGAATGGCCTGGACAA GACACGACTCCTCAAGAAAGATGCCCCCATCAGCCCCGCTTCTGTTGCATCTTCCAGCAGTACTCCTTCC TCCAAATCCAAAGAGCTTAGCCTTAATGAAAAGTCTACTACTCCTGTTTCAAAGTCCAATACCCCTACTC CACGAACTGATGCACCTACCCCTGGCAGTAATTCTACTCCTGGACTGAGGCCTGTACCTGGAAAGCCACC AGGTGTGGACCCCTTAGCATCAAGCCTGAGGACCCCGATGGCTGTACCTTGTCCATATCCAACCCCCTTT GGGATCGTGCCCCATGCTGGGATGAACGGAGAGCTGACCAGCCCCGGAGCCGCCTATGCTGGGCTACACA ACATCTCCCCCCAGATGAGTGCAGCTGCTGCTGCAGCTGCGGCAGCAGCAGCCTATGGAAGATCACCTGT GGTGGGATTTGATCCACACCATCACATGCGAGTGCCAGCAATACCTCCAAATCTAACAGGCATTCCAGGA GGAAAACCAGCATACTCCTTTCACGTCAGTGCCGATGGCCAGATGCAGCCTGTCCCTTTCCCCCCTGATG CCCTCATTGGACCAGGGATCCCCCGACACGCTCGACAGATCAACACCCTCAACCATGGGGAGGTGGTGTG TGCAGTGACCATCAGCAACCCCACAAGGCACGTGTACACAGGTGGCAAGGGCTGTGTTAAGGTCTGGGAC ATCAGCCACCCTGGCAACAAGAGCCCAGTCTCTCAGCTGGACTGTCTGAACAGGGATAACTACATCCGTT CCTGCAGATTGCTCCCTGATGGTCGCACCTTAATTGTTGGAGGGGAAGCCAGCACACTGTCCATCTGGGA CCTGGCAGCTCCAACTCCACGCATCAAGGCAGAGCTGACATCCTCAGCCCCTGCCTGCTATGCTCTGGCC ATCAGCCCCGACTCCAAGGTCTGCTTCTCATGCTGCAGCGACGGTAACATCGCAGTGTGGGATCTGCACA ACCAGACTCTGGTGAGGCAATTCCAGGGGCACACAGATGGAGCCAGCTGTATTGACATTTCTAATGATGG CACCAAGCTCTGGACAGGTGGTTTGGACAACACTGTGAGGTCCTGGGACCTGCGTGAAGGGCGGCAGCTG CAGCAACATGACTTCACCTCTCAGATCTTTTCATTGGGCTATTGCCCAACTGGAGAGTGGCTTGCAGTGG GGATGGAGAATAGCAATGTGGAAGTATTGCATGTCACCAAACCAGACAAATACCAGTTGCATCTTCATGA GAGCTGTGTGCTGTCACTCAAGTTTGCCCACTGTGGCAAATGGTTTGTAAGCACTGGAAAGGACAACCTT CTGAATGCTTGGAGGACGCCTTATGGGGCCAGCATATTCCAGTCCAAAGAATCCTCATCGGTGCTTAGCT GTGACATCTCTGTGGATGACAAGTACATTGTCACTGGCTCTGGGGACAAGAAAGCTACGGTTTATGAAGT TATTTATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302950
Insert Size	2250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302950.1, NP_001289879.1
RefSeq Size	4683 bp
RefSeq ORF	2250 bp
Locus ID	21888
Gene Summary	Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1.

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