Cars (NM_001252593) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CA3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228842 representing NM_001252593 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGTTCCTCCGCGGAGCAGGGTAAAGGCCGGCGAGTGCAGCCTCAGTGGTCCCCCCCAGCAGGGA CTGAGCCCTGCAGGCTCCGCCTCTATAATAGCCTCACCCGGAACAAGGATGTATTTATACCTCAAGATGG GAAGAAGGTGACGTGGTACTGTTGCGGGCCGACTGTCTATGATGCGTCTCACATGGGACATGCCAGGTCC TACATCTCCTTCGATATCCTGAGGAGGGTGCTGAGGGATTACTTCCAGTATGATGTCTTTTACTGCATGA ACATCACAGACATTGATGATAAGATCATCAGGCGGGCACGGCAGAACTACCTGTTTGAGCAGTATCGGGA GCAGAAACCCCCAGCCACCCAGCTCCTGAAGGATGTCCGTGATGCCATGAAGCCATTTTCAGTCAAGTTA AGTGAGACAACAGATCCCGACAAGAGGCAGATGCTGGAGCGGATCCAGAACTCTGTGAAACTTGCCACAG AACCACTGGAACAGGCTGTGCGATCCAGCCTCTCTGGAGAGGAAGTAGACAGCAAAGTACAGGTGTTGCT GGAGGAAGCAAAGGACTTGCTCTCTGACTGGCTGGATTCCACAGGTGGCAGTGAGGTGACTGACAACTCG ATTTTCTCCAAACTGCCCAAGTTCTGGGAAGAAGAGTTCCACAAAGACATGGAAGCCCTGAATGTTCTCC CTCCTGATGTCCTAACCCGTGTCAGTGAATATGTGCCAGAAATTGTGAACTTCGTCCAGAAGATTGTGGA CAATGGTTATGGTTATGCTTCAAATGGATCTGTTTACTTCGACACAGCCAAGTTTGCTGCTAGTGAAAAG CACTCCTATGGGAAGCTGGTGCCTGAGGCCGTTGGGGATCAGAAGGCACTTCAGGAAGGGGAAGGTGATC TGAGCATCTCTGCTGACCGCCTTAGTGAGAAACGCTCTCCCAATGACTTTGCCTTGTGGAAGGCCTCCAA GCCAGGCGAGCCATCCTGGCCTTGCCCCTGGGGAAAGGGTCGTCCCGGATGGCACATAGAGTGTTCTGCT ATGGCAGGCACGCTCCTGGGAGCCTCAATGGACATTCATGGTGGAGGGTTTGACCTCCGCTTCCCCCACC ATGACAATGAGCTGGCACAGTCGGAGGCCTACTTTGAAAATGACTGCTGGGTCAGGTACTTCTTGCACAC GGGCCACTTGACGATAGCAGGCTGCAAGATGTCCAAGTCACTGAAAAACTTTATCACCATTAAAGATGCC TTGAAGAAGCACTCAGCACGGCAGCTGCGACTGGCATTCCTCATGCACTCATGGAAAGACACACTGGACT ATTCCAGCAACACTATGGAGTCTGCTCTTCAGTATGAAAAATTCATGAACGAGTTTTTCTTAAATGTGAA AGACATCCTCCGAGCCCCTGTAGACATCACTGGCCAGTTTGAAAAGTGGGAAGCTGAAGAAGTGGAGCTA AATAAGAACTTCTATGGCAAGAAGACCGCAGTTCACGAAGCCTTGTGTGATAACATTGACACCCGGACTG TCATGGAAGAGATGCGGGCTTTGGTCAGTCAGTGCAACCTCTACATGGCAGCCAGGAAGGCTGAACGGAG GAGGCCCAACCGGGCTCTGCTGGAGAACATTGCCATGTACCTCACCCACATGCTGAAGATCTTTGGGGCC ATAGAGGAGGAGAGCCCCCTGGGGTTCCCAGTTGGTGGGCCTGGAACCAACCTGAACCTCGAGTCAACAG TCATGCCCTACCTTCAGGTGTTATCAGAATTCAGAGAGGGAGTACGAAAGATTGCCCGAGAGAAAAAAGT TCTTGAGGTTCTACAGCTCAGTGATGCCCTCCGGGATGACATCCTGCCTGAGCTTGGGGTCCGGTTTGAA GACCATGAAGGGCTGCCAACAGTGGTGAAGTTGGTGGACAGAGACACCTTACTGAAAGAGAAGGAAGGAA AGAAAAGGGCTGAAGAAGAGAAGAGGAGGAAGAAAGAGGAGGCGGCCAGAAAGAAACAGGAGCAAGAAGC AGCAAAGCTGGCCAAGATGAAGATACCACCCAGTGAGATGTTCCTGTCAGAAGTCAACAAGTATTCTAAA TTTGATGAAAATGGTCTGCCTACCCACGACACCGAAGGCAAAGAGCTGAGCAAGGGCCAGGCCAAGAAGC TGAAGAAGCTCTTTGAAGCCCAGGAGAAACTGTACAAGGAGTATCTGCAAATGTTACAGAATGGCAGCCT CCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252593
Insert Size	2247 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252593.1, NP_001239522.1
RefSeq Size	2987 bp
RefSeq ORF	2247 bp
Locus ID	27267
UniProt ID	Q9ER72
Gene Summary	Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate, in-frame, exon in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1.

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