Fign (NM_001267847) Mouse Untagged Clone

CAT#: MC228841

Fign (untagged) - Mouse fidgetin (Fign), transcript variant 3



  "NM_001267847" in other vectors (1)

CNY 7,130.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms fi; fidget
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228841 representing NM_001267847
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGTGGACGCCGGAGCATGCCCAGTGGCCAGAACAGCACTTTGACATCACCTCGACCACTCGGTCTC
CCGCCCACAAAGTTGAAGCCTATAGAGGTCATTTACAGCGCACCTACCAGTATGCCTGGGCGAATGATGA
CATATCTGCTCTGACTGCGTCCAACCTTCTGAAAAAATATGCAGAGAAGTATTCTGGCATTTTGGAAGGT
CCCGTAGACCGACCTGTACTCAGCAATTATTCAGACACACCATCAGGACTCGTGAATGGTCGGAAAAATG
ATAGCGAACCCTGGCAGCCTTCCTTGAATTCAGAAGCCGTTTATCCTATGAACTGTGTCCCGGATGTCAT
CACTGCCAGCAAAGCTGGTGTCAGCTCAGCCCTCCCTCCAGTAGACGTCTCGGCAAGTATAGGGAGCTCC
CCTGGGGTGGCCAGCAACCTGACAGAGCCAAGTTACTCAAGTAGTACCTGTGGAAGCCACACAGTACCTA
GTCTTCATGCGGGGCTCCCATCTCAGGAATATGCTCCGGGATACAACGGCTCATATTTGCATTCTACCTA
CAGCAGCCAGGCCACACCTGCACTTCCTTCGCCTCATCCGTCCCCGTTGCATAGTTCTGGGCTTTTGCAG
CCACCACCCCCTCCTCCTCCCCCACCAGCCCTGGTCCCAGGCTACAACGGGACTTCCAATCTCTCCAGTT
ACAGTTATCCCTCTGCTAGCTATCCTCCTCAGACTGCCGTGGGGTCTGGGTACAGCCCTGGCGGAGCACC
CCCTCCTCCTTCAGCATACCTGCCTTCAGGAATTCCTGCTCCCACCCCTCTGCCCCCCACTACCGTTCCT
GGCTACACCTACCAAGGTCATGGTTTGACACCCATTGCACCCTCTGCTCTGACCAACAATTCAGCAAGTT
CTCTCAAAAGGAAAGCTTTCTATATGGCAGGGCAAGGAGACATGGACTCCAGTTACGGAAATTACAGCTA
TGGCCAACAGAGATCGACACAGAGTCCTATGTACCGAATGCCCGACAACAGCATTTCAAACTCGAATCGG
GGGAATGGCTTTGACAGAAATGCTGAAACATCATCCTTAGCATTTAAGCCAACAAAGCAGCTGATGCCCT
CTGAGCAGCAAAGGAAATTCAGCAGCCAGTCCAGTAGGGCTCTGACCCCTCCTTCCTATAGTACTGCTAA
AAATTCATTGGGATCAAGGTCCAGTGAATCCTTTGGGAAGTATACGTCGCCAGTCATGAGTGAGCACGGA
GACGACCATCGGCAGCTCCTCGCTCATCCAATACAAGGTCCCGGACTCCGTGCAGCTACCTCATCCAACC
ACTCTGTGGACGAGCAACTGAAGAATACTGACACACACCTCATTGACCTGGTCACCAATGAGATCATCAC
CCAAGGACCTCCTGTGGACTGGAGCGACATAGCTGGTCTTGACCTGGTGAAGGCGGTTATTAAGGAGGAG
GTTTTGTGGCCAGTGCTGAGGTCAGATGCATTCAGTGGGCTGACCGCCTTACCTAGAAGCATCCTTCTCT
TCGGACCTCGGGGTACAGGCAAAACACTTTTGGGCAGATGCATAGCTAGTCAGCTAGGGGCCACGTTTTT
CAAAATTGCCGGTTCCGGACTCGTTGCAAAGTGGATAGGAGAAGCGGAGAAAATCATCCACGCCTCTTTT
CTTGTGGCCAGGTGTCGCCAGCCCTCGGTGATTTTTGTTAGTGACATTGACATGCTTCTCTCCTCTCAAG
TGAGCGAGGAACACAGTCCAGTCAGTCGGATGAGAACCGAATTTCTGATGCAGCTGGACACTGTACTGAC
TTCGGCTGAGGACCAAATCGTAGTCATTTGTGCCACCAGTAAACCAGAAGAAATAGATGAATCTCTTCGG
AGGTACTTCATGAAAAGACTTTTAATCCCACTTCCTGACAGCACAGCGAGGCACCAGATAATAGTACAGC
TGCTCACACAGCACAATTACTGTCTCAATGACAAGGAGTTTGCACTGCTTGTCCAGCGCACAGAAGGCTT
TTCTGGACTAGACGTGGCTCATTTGTGTCAGGAAGCAGCAGTGGGACCTCTCCATGCTATGCCAGCTACA
GACCTTTCAGCCATTATGCCCAGCCAGTTAAGGCCCGTTACATATCAAGACTTTGAAAATGCTTTCTGCA
AGATTCAGCCTAGCATATCTCAAAAAGAGCTTGATATGTATGTTGAATGGAACAAAATGTTTGGTTGCAG
TCAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001267847
Insert Size 2247 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001267847.1, NP_001254776.1
RefSeq Size 9798 bp
RefSeq ORF 2247 bp
Locus ID 60344
UniProt ID Q9ERZ6
Gene Summary ATP-dependent microtubule severing protein. Severs microtubules along their length and depolymerizes their ends, primarily the minus-end, suppressing microtubule growth from and attachment to centrosomes. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) has an alternate segment in the 5' coding region, which results in a downstream in-frame start codon, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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