Fign (NM_001267847) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	fi; fidget
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228841 representing NM_001267847 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTGGACGCCGGAGCATGCCCAGTGGCCAGAACAGCACTTTGACATCACCTCGACCACTCGGTCTC CCGCCCACAAAGTTGAAGCCTATAGAGGTCATTTACAGCGCACCTACCAGTATGCCTGGGCGAATGATGA CATATCTGCTCTGACTGCGTCCAACCTTCTGAAAAAATATGCAGAGAAGTATTCTGGCATTTTGGAAGGT CCCGTAGACCGACCTGTACTCAGCAATTATTCAGACACACCATCAGGACTCGTGAATGGTCGGAAAAATG ATAGCGAACCCTGGCAGCCTTCCTTGAATTCAGAAGCCGTTTATCCTATGAACTGTGTCCCGGATGTCAT CACTGCCAGCAAAGCTGGTGTCAGCTCAGCCCTCCCTCCAGTAGACGTCTCGGCAAGTATAGGGAGCTCC CCTGGGGTGGCCAGCAACCTGACAGAGCCAAGTTACTCAAGTAGTACCTGTGGAAGCCACACAGTACCTA GTCTTCATGCGGGGCTCCCATCTCAGGAATATGCTCCGGGATACAACGGCTCATATTTGCATTCTACCTA CAGCAGCCAGGCCACACCTGCACTTCCTTCGCCTCATCCGTCCCCGTTGCATAGTTCTGGGCTTTTGCAG CCACCACCCCCTCCTCCTCCCCCACCAGCCCTGGTCCCAGGCTACAACGGGACTTCCAATCTCTCCAGTT ACAGTTATCCCTCTGCTAGCTATCCTCCTCAGACTGCCGTGGGGTCTGGGTACAGCCCTGGCGGAGCACC CCCTCCTCCTTCAGCATACCTGCCTTCAGGAATTCCTGCTCCCACCCCTCTGCCCCCCACTACCGTTCCT GGCTACACCTACCAAGGTCATGGTTTGACACCCATTGCACCCTCTGCTCTGACCAACAATTCAGCAAGTT CTCTCAAAAGGAAAGCTTTCTATATGGCAGGGCAAGGAGACATGGACTCCAGTTACGGAAATTACAGCTA TGGCCAACAGAGATCGACACAGAGTCCTATGTACCGAATGCCCGACAACAGCATTTCAAACTCGAATCGG GGGAATGGCTTTGACAGAAATGCTGAAACATCATCCTTAGCATTTAAGCCAACAAAGCAGCTGATGCCCT CTGAGCAGCAAAGGAAATTCAGCAGCCAGTCCAGTAGGGCTCTGACCCCTCCTTCCTATAGTACTGCTAA AAATTCATTGGGATCAAGGTCCAGTGAATCCTTTGGGAAGTATACGTCGCCAGTCATGAGTGAGCACGGA GACGACCATCGGCAGCTCCTCGCTCATCCAATACAAGGTCCCGGACTCCGTGCAGCTACCTCATCCAACC ACTCTGTGGACGAGCAACTGAAGAATACTGACACACACCTCATTGACCTGGTCACCAATGAGATCATCAC CCAAGGACCTCCTGTGGACTGGAGCGACATAGCTGGTCTTGACCTGGTGAAGGCGGTTATTAAGGAGGAG GTTTTGTGGCCAGTGCTGAGGTCAGATGCATTCAGTGGGCTGACCGCCTTACCTAGAAGCATCCTTCTCT TCGGACCTCGGGGTACAGGCAAAACACTTTTGGGCAGATGCATAGCTAGTCAGCTAGGGGCCACGTTTTT CAAAATTGCCGGTTCCGGACTCGTTGCAAAGTGGATAGGAGAAGCGGAGAAAATCATCCACGCCTCTTTT CTTGTGGCCAGGTGTCGCCAGCCCTCGGTGATTTTTGTTAGTGACATTGACATGCTTCTCTCCTCTCAAG TGAGCGAGGAACACAGTCCAGTCAGTCGGATGAGAACCGAATTTCTGATGCAGCTGGACACTGTACTGAC TTCGGCTGAGGACCAAATCGTAGTCATTTGTGCCACCAGTAAACCAGAAGAAATAGATGAATCTCTTCGG AGGTACTTCATGAAAAGACTTTTAATCCCACTTCCTGACAGCACAGCGAGGCACCAGATAATAGTACAGC TGCTCACACAGCACAATTACTGTCTCAATGACAAGGAGTTTGCACTGCTTGTCCAGCGCACAGAAGGCTT TTCTGGACTAGACGTGGCTCATTTGTGTCAGGAAGCAGCAGTGGGACCTCTCCATGCTATGCCAGCTACA GACCTTTCAGCCATTATGCCCAGCCAGTTAAGGCCCGTTACATATCAAGACTTTGAAAATGCTTTCTGCA AGATTCAGCCTAGCATATCTCAAAAAGAGCTTGATATGTATGTTGAATGGAACAAAATGTTTGGTTGCAG TCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001267847
Insert Size	2247 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001267847.1, NP_001254776.1
RefSeq Size	9798 bp
RefSeq ORF	2247 bp
Locus ID	60344
UniProt ID	Q9ERZ6
Gene Summary	ATP-dependent microtubule severing protein. Severs microtubules along their length and depolymerizes their ends, primarily the minus-end, suppressing microtubule growth from and attachment to centrosomes. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) has an alternate segment in the 5' coding region, which results in a downstream in-frame start codon, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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