Golga1 (NM_001290649) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	0710001G09Rik; 2210418B03Rik; AW107649; awag; Golgi97; golgin-97
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228810 representing NM_001290649 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGCAAAACTAAAGAAGAAAATTGCAGAAGAGACTGCTGTTGCTCAGAGACCAGGCGGCACAACAA GGATCCCACGCTCTGTGAGCAAGGAGTCTGTGGCCTCCATGGGAGCTGACTCTGGAGATGATTTTGCTTC CGATGGTAGCAGCTCCAGAGAAGATCTCTCATCCCAGCTTTTGAGAAGAAATGAACAGATACGGAAGTTA GAGGCCAGACTTTCTGCTTCCATGCGGAAATTTCAAGAACAGAATGAAACATTCCAAGCCAGCAGAGCCA AAATGGCAGAAGGACTGGCTTTGGCTTTAGCCAGGAAGGACCAGGAATGGTCAGAAAAGATGGAACAGCT TGAAAAGGATAAAAGGTTTCTAACATCTCAGCTGCAAGAAGTAAAGAACCAGAGCTTGAGCCTTTTTCAA AAAAGAGATGAAATTGATGAATTAGAGGGGTTCCAGCAGCAGGAAATCAGCAAAGTAAAACACATGCTTT TAAAAAAAGAAGAATGCTTGGGGAAGATGGAGCAAGAATTGGATGCACGAACCAGGGAACTTAACCGTAC TCAGGAGGAGTTGGTGACTTCTAATCAGTTGTCATCTGACTTAAACGAGCGGCTAGAAGAATTGCAGAGA CACTGCTCAACGCTGGAAGAGCAGAGAGATCACTTGACAGCTTCAAAAGCAGGTGCAGAACATAAGATTG TAGTCCTGGAACAAAAGGAACAAGAGCTCCAGGCGATCATCCAGCAGCATTCCATTGACCTACAAAAGGT CACTGCTGAAACGCAAGAAAAAGAAAAAGTCATCACACATCTACAAGAGAAGGTTATATTCTTGGAAAAG AGACTAGAACAGAATTTATCAGGAGAAGATCATGTACAAGAACTCTTGAAAGAGAAAACAGTTGCTGAAC AGAATCTGGAAGATACTAGGCAGCAGCTCTTGGCTGCCAGGAACAGCCACACCAAGGCACTTTACCTCCT GGAGACTCGGGTGAAGGACCTAGAGCGGAGTCTACAGGCTGCTGAAGAACAGCTAAGCCAGAGTAGAAAT GTTGTGGCTGACCAGGAAGCCCAGATCCAGAAGCTTATCACTACAAACCAGGAGAACAGCCTTTCCCAGC AGCAGGTTCTTGCCCTGGAACAGCATTGCAGGGAGCGCATCCATGCACTGGAGGCCCAAATTGAAGCTCT GGAACAAACACGGGTAGCTGACCAGATAGCCTCAGAGCAGGGAATGCTACAACTGCAGCAGGAAAATGTG GCCCTTAAAGAAAGCAGAAATGAATGTGAACATTCCTTACAACATCACCAATTGGAACTAAAGAAACTAA AGGATGAATGGAGCCAAAGAGAAATTGTGAGTGTAGCAATGGCTCAAGCCCTGGAGGAGGTGCGGAAACA GAGAGAAGAGTTCCAGCAACAGGCCACTGAGTTGACAGCCATCATAGAAGAGAAAAATCAGAGCCTGTGT GAAAAGGATGAGGCACTTCTCCAGAAGGAGCAGGAACTTCGACAGCTGGAAAAAGGTCACAGTTCTGCCC TGCTGCAGATGCACAAACTGCAGAGGGAACTAGAGGCCTTGAAGACTTGCAAGGCTCAGGAGGCAATGCC AGCCACAACAGGAGAGGACTGCCTGCCACTGCAGGGCCAGGAGCCACTTGTCATTTCAAAAGCCATGCAG AATTCTGAGTATGAGCTTCCAGCTGCAGAAGGAACTCCAAACGGTGAGGTTGGAGCCAGTGATCTAAAGC AGCTACAGAAAGAAAAGCAGGACTTAGAACAGCAACTTATCGAGAAAAATAAGATTATGAAGCAAATGCA GCAGAGGATGCTGGAACTTAAGAAGACTCTACAGAAAGAGTTGAAAATCAGACCTGACAGTGAGCTTTTT GAGGTCCGGGAGAAGACAGGCCCTGAGATACCAAATATGGCCCCTTCTGTCACTAATAATACTGACCTGA CTGATGCCCGAGAGATCAACTTTGAATACCTTAAACATGTTGTTTTAAAATTCATGTCCTGTCGAGAATC TGAGGCTTTTCATCTCATAAAAGCAGTGTCAGTGCTGCTGAATTTCTCACAAGAAGAGGAGAATATGCTC AAAGAGACTCTGGAATATAAGATGTCTTGGTTTGGATCCAAACCAACTCCCAAGGGCAGCATCCGGCCAT CTATCTCCAACCCTCGGATACCATGGTCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290649
Insert Size	2202 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290649.1, NP_001277578.1
RefSeq Size	4793 bp
RefSeq ORF	2202 bp
Locus ID	76899
UniProt ID	Q9CW79
Gene Summary	Involved in vesicular trafficking at the Golgi apparatus level. Involved in endosome-to-Golgi trafficking.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.

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