Csf3r (NM_001252651) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Cd114; Csfgr; G-CSF-R; G-CSFR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228796 representing NM_001252651 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTAGGGCTGGGAGCCTGCACCCTGACTGGAGTTACCCTGATCTTCTTGCTACTCCCCAGAAGTCTGG AGAGCTGTGGACACATCGAGATTTCACCCCCTGTTGTCCGCCTGGGGGACCCTGTCCTGGCCTCTTGCAC CATCAGCCCAAACTGCAGCAAACTGGACCAACAGGCAAAGATCTTATGGAGACTGCAAGATGAGCCCATC CAACCTGGGGACAGACAGCATCATCTGCCTGATGGGACCCAAGAGTCCCTCATCACTCTGCCTCACTTGA ACTACACCCAGGCCTTCCTCTTCTGCTTAGTGCCATGGGAAGACAGCGTCCAACTCCTGGATCAAGCTGA GCTTCACGCAGGCTATCCCCCTGCCAGCCCCTCAAACCTATCCTGCCTCATGCACCTCACCACCAACAGC CTGGTCTGCCAGTGGGAGCCAGGTCCTGAGACCCACCTGCCCACCAGCTTCATCCTAAAGAGCTTCAGGA GCCGCGCCGACTGTCAGTACCAAGGGGACACCATCCCGGATTGTGTGGCAAAGAAGAGGCAGAACAACTG CTCCATCCCCCGAAAAAACTTGCTCCTGTACCAGTATATGGCCATCTGGGTGCAAGCAGAGAATATGCTA GGGTCCAGCGAGTCCCCAAAGCTGTGCCTCGACCCCATGGATGTTGCCCCCACCATCAGACTGGACACGT GGTGTCAGAAGAAGCAACTAGATCCAGGGACAGTGAGTGTGCAGCTGTTCTGGAAGCCAACGCCCCTGCA GGAAGACAGTGGACAGATCCAGGGCTACCTGCTGTCCTGGAGTTCCCCAGATCATCAAGGGCAGGACATA CACCTTTGCAACACCACGCAGCTCAGCTGTATCTTCCTCCTGCCCTCAGAGGCCCAGAACGTGACCCTTG TGGCCTACAACAAAGCAGGGACCTCTTCACCTACTACAGTGGTTTTCCTGGAGAACGAAGGTCCAGCTGT GACCGGACTCCATGCCATGGCCCAAGACCTTAACACCATCTGGGTAGACTGGGAAGCCCCCAGCCTTCTG CCTCAGGGCTATCTCATTGAGTGGGAAATGAGTTCTCCCAGCTACAATAACAGCTATAAGTCCTGGATGA TAGAACCTAACGGGAACATCACTGGAATTCTGTTAAAGGACAACATAAATCCCTTTCAGCTCTACAGAAT TACAGTGGCTCCCCTGTACCCAGGCATCGTGGGACCTCCTGTAAATGTCTACACCTTCGCTGGAGAGAGA GCTCCTCCTCATGCTCCAGCGCTGCATCTAAAGCATGTTGGCACAACCTGGGCACAGCTGGAGTGGGTAC CTGAGGCCCCTAGGCTGGGGATGATACCCCTCACCCACTACACCATCTTCTGGGCCGATGCTGGGGACCA CTCCTTCTCCGTCACCCTGAACATCTCCCTCCATGACTTTGTCCTGAAGCACCTGGAGCCCGCCAGTTTG TATCATGTCTACCTCATGGCCACCAGTCGGGCAGGGTCCACCAACAGTACAGGCCTTACCCTGAGGACCC TAGATCCATCTGACTTAAACATTTTCCTGGGCATACTTTGCTTAGTACTCTTGTCCACTACCTGTGTAGT GACCTGGCTCTGCTGCAAACGCAGAGGAAAGACTTCCTTCTGGTCAGATGTGCCAGACCCAGCCCACAGT AGCCTGAGCTCCTGGTTGCCCACCATCATGACAGAGGAAACCTTCCAGTTACCCAGCTTCTGGGACTCCA GCGTGCCATCAATCACCAAGATCACTGAACTGGAGGAAGACAAGAAACCGACCCACTGGGATTCCGAAAG CTCTGGGAATGGTAGCCTTCCAGCCCTGGTTCAGGCCTATGTGCTCCAAGGAGATCCAAGAGAAATTTCC AACCAGTCCCAGCCTCCCTCTCGCACTGGTGACCAGGTCCTCTATGGTCAGGTGCTTGAGAGCCCCACCA GCCCAGGAGTAATGCAGTACATTCGCTCTGACTCCACTCAGCCCCTCTTGGGGGGCCCCACCCCTAGCCC TAAATCTTATGAAAACATCTGGTTCCATTCAAGACCCCAGGAGACCTTTGTGCCCCAACCTCCAAACCAG GAAGATGACTGTGTCTTTGGGCCTCCATTTGATTTTCCCCTCTTTCAGGGGCTCCAGGTCCATGGAGTTG AAGAACAAGGGGGTTTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252651
Insert Size	2190 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252651.1, NP_001239580.1
RefSeq Size	3583 bp
RefSeq ORF	2190 bp
Locus ID	12986
Gene Summary	Receptor for granulocyte colony-stimulating factor (CSF3). In addition it may function in some adhesion or recognition events at the cell surface.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks two exons in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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