Hgf (NM_001289459) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C230052L06Rik; HGF/S; HGF/SF; NK; NK1; NK2; SF; SF/HG; SF/HGF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228791 representing NM_001289459 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTGGGGGACCAAACTTCTGCCGGTCCTGTTGCTGCAGCATGTCCTCCTGCACCTCCTCCTGCTTC ATGTCGCCATCCCCTATGCAGAAGGACAGAAGAAAAGAAGAAATACACTTCATGAATTTAAAAAGTCAGC AAAAACTACTCTTACCAAGGAAGACCCATTACTGAAGATTAAAACCAAAAAAGTGAACTCTGCAGATGAG TGTGCCAACAGGTGTATCAGGAACAGGGGCTTTACGTTCACTTGCAAGGCCTTCGTTTTTGATAAGTCAA GAAAACGATGCTACTGGTATCCTTTCAATAGTATGTCAAGTGGAGTGAAAAAAGGGTTTGGCCATGAATT TGACCTCTATGAAAACAAAGACTATATTAGAAACTGCATCATTGGTAAAGGAGGCAGCTATAAAGGGACG GTATCCATCACTAAGAGTGGCATCAAATGCCAGCCTTGGAATTCCATGATCCCCCATGAACACAGCTTTT TGCCTTCGAGCTATCGCGGTAAAGACCTACAGGAAAACTACTGTCGAAATCCTCGAGGGGAAGAAGGGGG ACCCTGGTGTTTCACAAGCAATCCAGAGGTACGCTACGAAGTCTGTGACATTCCTCAGTGTTCAGAAGTT GAATGCATGACCTGCAATGGTGAAAGCTACAGAGGTCCCATGGATCACACAGAATCAGGCAAGACTTGTC AGCGCTGGGACCAGCAGACACCACACCGGCACAAGTTCTTGCCAGAAAGATATCCCGACAAGGGCTTTGA TGATAATTATTGCCGCAATCCTGATGGCAAGCCGAGGCCATGGTGCTACACTCTTGACCCTGACACCCCT TGGGAGTATTGTGCAATTAAAACGTGCGCTCACAGTGCTGTGAATGAGACTGATGTCCCTATGGAAACAA CTGAATGCATTCAAGGCCAAGGAGAAGGTTACAGGGGAACCAGCAATACCATTTGGAATGGAATTCCCTG TCAGCGTTGGGATTCGCAGTACCCTCACAAGCATGATATCACTCCCGAGAACTTCAAATGCAAGGACCTT AGAGAAAATTATTGCCGCAATCCAGATGGGGCTGAATCACCATGGTGTTTTACCACTGACCCAAACATCC GAGTTGGCTACTGCTCTCAAATTCCCAAGTGTGACGTGTCAAGTGGACAAGATTGTTATCGTGGCAATGG GAAAAATTACATGGGCAACTTATCCAAAACAAGGTCTGGACTTACATGTTCCATGTGGGACAAGAATATG GAGGATTTACACCGTCATATCTTCTGGGAGCCAGATGCTAGCAAATTGAATAAGAATTACTGCCGGAATC CTGATGATGATGCCCATGGACCTTGGTGCTACACGGGGAATCCTCTTATTCCTTGGGATTATTGCCCTAT TTCCCGTTGTGAAGGAGATACTACACCTACAATTGTCAATTTGGACCATCCTGTAATATCCTGTGCCAAA ACAAAACAACTGCGGGTTGTAAATGGCATTCCAACACAAACAACAGTAGGGTGGATGGTTAGTTTGAAAT ACAGAAATAAACATATCTGTGGAGGATCATTGATAAAGGAAAGTTGGGTTCTTACTGCAAGACAATGTTT TCCAGCCAGAAACAAAGACTTGAAAGACTATGAAGCTTGGCTTGGCATCCACGATGTTCATGAGAGAGGC GAGGAGAAGCGCAAGCAGATCTTAAACATTTCCCAGCTGGTCTATGGTCCTGAAGGCTCAGACTTGGTTT TACTGAAGCTTGCTCGACCTGCAATCCTGGATAACTTTGTCAGTACAATTGATTTACCTAGTTATGGTTG TACAATCCCTGAAAAGACCACTTGCAGTATTTACGGCTGGGGCTACACTGGATTGATCAACGCGGATGGT TTATTACGAGTAGCTCATCTGTATATTATGGGGAATGAGAAATGCAGTCAGCACCATCAAGGCAAGGTGA CTTTGAATGAGTCTGAGTTATGTGCTGGGGCTGAAAAGATTGGATCAGGACCATGTGAGGGAGATTATGG TGGCCCACTCATTTGTGAACAACACAAAATGAGAATGGTTCTTGGTGTCATTGTTCCTGGTCGTGGATGT GCCATCCCAAATCGTCCTGGTATTTTTGTTCGAGTAGCATATTATGCAAAATGGATACACAAAGTAATTT TGACATACAAGTTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289459
Insert Size	2187 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289459.1, NP_001276388.1
RefSeq Size	2827 bp
RefSeq ORF	2187 bp
Locus ID	15234
UniProt ID	Q08048
Gene Summary	This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the hepatocyte growth factor alpha and beta chains, which heterodimerize to form the mature active protein. Although this protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Homozygous knockout mice for this gene exhibit embryonic lethality due to impaired development of the placenta and liver. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same isoform (1).

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