Eef2k (NM_001267710) Mouse Untagged Clone

CAT#: MC228776

Eef2k (untagged) - Mouse eukaryotic elongation factor-2 kinase (Eef2k), transcript variant 2



  "NM_001267710" in other vectors (1)

CNY 6,940.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms C86191; eEF-2K
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228776 representing NM_001267710
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGACGAAGACCTCATCTTCTGCCTGGAAGGTGTTGACGGTGGCAGGTGCTCCCGAGCTGGCCACA
ATGCGGACTCTGACACAGACAGTGACGATGATGAGGGCTATTTCATCTGCCCCATCACTGATGACCACAT
GTCCAATCAGAATGTCAGCTCCAAAGTCCAGAGCTACTATAGCAACCTAACAAAAACAGAGTGCGGCTCC
ACAGGGTCACCAGCCAGCTCCTTCCACTTCAAGGAAGCCTGGAAGCATGCGATCGAGAAAGCCAAGCACA
TGCCTGACCCCTGGGCTGAATTCCATCTCGAGGACATCGCCACAGAACATGCTACTCGGCACAGGTACAA
CGCTGTCACCGGGGAATGGCTGAAAGACGAGGTTCTGATCAAGATGGCGTCTCAGCCCTTCGGCCGTGGA
GCAATGAGGGAGTGCTTCAGGACGAAGAAACTCTCCAACTTCTTGCACGCCCAGCAATGGAAGGGGGCCT
CCAACTACGTGGCCAAGCGCTACATCGAGCCGGTGGACAGGAGCGTGTACTTTGAGGATGTGCAGCTCCA
GATGGAGGCGAAGCTCTGGGGGGAGGATTACAATCGGCACAAGCCCCCCAAGCAGGTGGATATCATGCAG
ATGTGCATCATTGAGCTAAAGGACAGACCAGGCCAGCCCCTCTTCCACTTGGAGCACTACATTGAGGGCA
AGTACATCAAGTACAATTCCAACTCAGGCTTTGTCCGTGATGACAACATCCGACTAACCCCACAGGCCTT
CAGCCATTTCACATTTGAGCGTTCTGGTCATCAGCTGATTGTAGTGGACATCCAGGGTGTGGGTGACCTT
TATACCGACCCACAGATCCACACTGAGAAAGGCACTGACTTTGGAGATGGTAACCTTGGTGTCCGGGGAA
TGGCTCTCTTCTTCTACTCTCATGCCTGCAACCGGATTTGTCAGAGCATGGGCCTTACGCCCTTTGACCT
CTCCCCACGGGAACAGGATGCGGTGAATCAGAGCACCAGGCTATTGCAATCAGCCAAGACCATCTTGAGG
GGGACAGAGGAGAAGTGTGGGAGTCCCCGCATAAGGACACTCTCTAGCAGCCGGCCCCCTTTGCTCCTTC
GCCTGTCAGAGAACTCCGGGGATGAGAACATGAGTGACGTGACCTTTGACTCTCTGCCTTCCTCCCCGTC
TTCAGCTACACCACACAGCCAGAAACTGGACCACCTCCATTGGCCAGTGTTTGGTGACCTCGATAACATG
GGCCCTAGAGACCATGACCGCATGGACAATCACCGGGACTCTGAGAATAGTGGGGACAGTGGGTATCCAA
GCGAGAAGCGAAGTGACCTGGATGATCCTGAGCCCCGAGAACACGGCCACTCCAACGGCAACCGAAGGCA
TGAATCTGACGAGGATAGCCTGGGCAGCTCTGGACGGGTCTGTGTGGAGACGTGGAACCTGCTCAATCCC
TCCCGCCTGCACCTGCCGAGGCCCTCGGCCGTGGCCCTAGAAGTGCAGAGGCTAAATGCCCTGGACCTTG
GAAGGAAAATCGGGAAGTCTGTTTTGGGGAAGGTCCATTTGGCCATGGTGCGATACCACGAGGGCGGGCG
CTTCTGCGAGAAGGATGAGGAGTGGGATCGAGAGTCAGCCATCTTCCATCTGGAGCATGCAGCTGACCTG
GGAGAACTGGAGGCCATCGTGGGCCTAGGCCTCATGTACTCTCAGCTGCCCCACCACATCCTGGCTGATG
TCTCTCTGAAGGAGACAGAGGAGAACAAGACAAAAGGCTTTGATTACTTACTGAAGGCGGCAGAAGCTGG
TGACAGGCATTCCATGATTTTAGTGGCCCGAGCTTTTGACACTGGCCTGAACCTCAGCCCAGACAGGTGT
CAAGACTGGTCGGAAGCCTTGCACTGGTACAACACAGCCCTGGAGACAACAGACTGCGATGAAGGCGGGG
AGTACGATGGGATACAGGACGAGCCCCAGTACGCACTGCTGGCCAGGGAGGCGGAGATGCTGCTCACCGG
GGGATTTGGACTGGACAAGAACCCCCAAAGATCAGGAGATTTGTACACCCAGGCAGCTGAGGCAGCAATG
GAAGCCATGAAGGGCCGGCTAGCCAACCAGTACTACGAGAAGGCGGAAGAGGCCTGGGCCCAGATGGAGG
AATAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001267710
Insert Size 2175 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001267710.1, NP_001254639.1
RefSeq Size 6282 bp
RefSeq ORF 2175 bp
Locus ID 13631
UniProt ID O08796
Gene Summary Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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