Eef2k (NM_001267710) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C86191; eEF-2K
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228776 representing NM_001267710 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACGAAGACCTCATCTTCTGCCTGGAAGGTGTTGACGGTGGCAGGTGCTCCCGAGCTGGCCACA ATGCGGACTCTGACACAGACAGTGACGATGATGAGGGCTATTTCATCTGCCCCATCACTGATGACCACAT GTCCAATCAGAATGTCAGCTCCAAAGTCCAGAGCTACTATAGCAACCTAACAAAAACAGAGTGCGGCTCC ACAGGGTCACCAGCCAGCTCCTTCCACTTCAAGGAAGCCTGGAAGCATGCGATCGAGAAAGCCAAGCACA TGCCTGACCCCTGGGCTGAATTCCATCTCGAGGACATCGCCACAGAACATGCTACTCGGCACAGGTACAA CGCTGTCACCGGGGAATGGCTGAAAGACGAGGTTCTGATCAAGATGGCGTCTCAGCCCTTCGGCCGTGGA GCAATGAGGGAGTGCTTCAGGACGAAGAAACTCTCCAACTTCTTGCACGCCCAGCAATGGAAGGGGGCCT CCAACTACGTGGCCAAGCGCTACATCGAGCCGGTGGACAGGAGCGTGTACTTTGAGGATGTGCAGCTCCA GATGGAGGCGAAGCTCTGGGGGGAGGATTACAATCGGCACAAGCCCCCCAAGCAGGTGGATATCATGCAG ATGTGCATCATTGAGCTAAAGGACAGACCAGGCCAGCCCCTCTTCCACTTGGAGCACTACATTGAGGGCA AGTACATCAAGTACAATTCCAACTCAGGCTTTGTCCGTGATGACAACATCCGACTAACCCCACAGGCCTT CAGCCATTTCACATTTGAGCGTTCTGGTCATCAGCTGATTGTAGTGGACATCCAGGGTGTGGGTGACCTT TATACCGACCCACAGATCCACACTGAGAAAGGCACTGACTTTGGAGATGGTAACCTTGGTGTCCGGGGAA TGGCTCTCTTCTTCTACTCTCATGCCTGCAACCGGATTTGTCAGAGCATGGGCCTTACGCCCTTTGACCT CTCCCCACGGGAACAGGATGCGGTGAATCAGAGCACCAGGCTATTGCAATCAGCCAAGACCATCTTGAGG GGGACAGAGGAGAAGTGTGGGAGTCCCCGCATAAGGACACTCTCTAGCAGCCGGCCCCCTTTGCTCCTTC GCCTGTCAGAGAACTCCGGGGATGAGAACATGAGTGACGTGACCTTTGACTCTCTGCCTTCCTCCCCGTC TTCAGCTACACCACACAGCCAGAAACTGGACCACCTCCATTGGCCAGTGTTTGGTGACCTCGATAACATG GGCCCTAGAGACCATGACCGCATGGACAATCACCGGGACTCTGAGAATAGTGGGGACAGTGGGTATCCAA GCGAGAAGCGAAGTGACCTGGATGATCCTGAGCCCCGAGAACACGGCCACTCCAACGGCAACCGAAGGCA TGAATCTGACGAGGATAGCCTGGGCAGCTCTGGACGGGTCTGTGTGGAGACGTGGAACCTGCTCAATCCC TCCCGCCTGCACCTGCCGAGGCCCTCGGCCGTGGCCCTAGAAGTGCAGAGGCTAAATGCCCTGGACCTTG GAAGGAAAATCGGGAAGTCTGTTTTGGGGAAGGTCCATTTGGCCATGGTGCGATACCACGAGGGCGGGCG CTTCTGCGAGAAGGATGAGGAGTGGGATCGAGAGTCAGCCATCTTCCATCTGGAGCATGCAGCTGACCTG GGAGAACTGGAGGCCATCGTGGGCCTAGGCCTCATGTACTCTCAGCTGCCCCACCACATCCTGGCTGATG TCTCTCTGAAGGAGACAGAGGAGAACAAGACAAAAGGCTTTGATTACTTACTGAAGGCGGCAGAAGCTGG TGACAGGCATTCCATGATTTTAGTGGCCCGAGCTTTTGACACTGGCCTGAACCTCAGCCCAGACAGGTGT CAAGACTGGTCGGAAGCCTTGCACTGGTACAACACAGCCCTGGAGACAACAGACTGCGATGAAGGCGGGG AGTACGATGGGATACAGGACGAGCCCCAGTACGCACTGCTGGCCAGGGAGGCGGAGATGCTGCTCACCGG GGGATTTGGACTGGACAAGAACCCCCAAAGATCAGGAGATTTGTACACCCAGGCAGCTGAGGCAGCAATG GAAGCCATGAAGGGCCGGCTAGCCAACCAGTACTACGAGAAGGCGGAAGAGGCCTGGGCCCAGATGGAGG AATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001267710
Insert Size	2175 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001267710.1, NP_001254639.1
RefSeq Size	6282 bp
RefSeq ORF	2175 bp
Locus ID	13631
UniProt ID	O08796
Gene Summary	Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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