Jade3 (NM_001289684) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5730598B06; AI851988; AW544806; mKIAA0215; Phf16
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228763 representing NM_001289684 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTTTTGTCCGGCCCCGGAAATATATCCGTTGCTCCAGCCCAGAGTCTGCAGAACCTGGCTATATCA ATACCCTGGAGCAAGCAGCATCTACATGCCGCTATGACCTAGATGACATGGATATCTTTTGGCTTCAGGA ACTCAATGAAGACCTTGGAGAAATGGGTTATGGCCCAATTGATGAGACTCTTATGGAAAAGACAATAGAA GTTCTGGAACGCCACTGCCATGAAAATATGAACCATGCTATTGAAACAGTGGAAGGGCTAGGAATAGAAT ATGATGAAGATGTGATCTGTGATGTGTGCCGGTCTCCAGACAGTGAAGAAGGGAATGATATGGTATTCTG TGATAAGTGTAACGTCTGTGTACATCAGGCTTGCTATGGCATTCTCAAGATTCCAGAAGGTAGTTGGCTG TGCCGCTCCTGTGTCCTGGGCATTTATCCACAGTGTGTTTTATGTCCTAAGAAAGGTGGAGCCATGAAGA CCACCAGAACAGGGACTAAATGGGCTCATGTCAGCTGTGCCCTGTGGATTCCAGAAGTCAGTATCGCTTG TCCTGAGAGAATGGAGCCTGTCACAAAAATCTCCCATATCCCACCTAGCCGGTGGGCCTTAGTTTGCAAC CTGTGCAAGTTGAAGACAGGGGCTTGTATTCAGTGCTCTGTGAAAAGCTGTATCACTGCCTTTCATGTCA CCTGTGCCTTTGAGCACGGCCTGGAAATGAAGACCATCCTAGATGAGGGGGATGAAGTAAAGTTCAAGTC ATTTTGCCTCAAGCATAGCCAAAACAAGCCAAAACTTGGGGACGCCGAGTACCACCACCACAGAGTCGCC GAGCAGAGCCAGGCAAAAAGTGAGAAAACCAGCCTACGGGCACAGAAGCTTCGTGAACTAGAAGAAGAAT TCTATACCTTAGTCCAGGTAGAAGATGTTGCCAAAGAGATGGAGTTATCTGCGTTCACTGTCGATTTTAT CTATAACTACTGGAAACTAAAGCGCAAAAGCAACTTCAATAAGCCATTAATTCCCCCAAAAGAAGAGGAA GAAAATGGCTTGGTTCAGCCAAAAGAAGAAAGCATTCACACTCGGATGAGAATGTTTATGCATCTGCGGC AGGACCTTGAGAGGGTCCGAAATCTGTGCTACATGATAAGCAGACGAGAGAAGCTGAAACTATCACACAC CAAAGTGCAGGAACAGATTTTTGGTTTGCAAGTCCAGCTTATCAATGAAGAAATTACAGAAGGACTTTCT TTGACAAATGCACTAGAGAACTCACTGTTTTATCCACCACCACGAATTACCTTAAAGTTAAAAATGCCTA AATCAACCTCAGAAGATTGCAAAGACAGCTCCACAGAAACTGAACACCAACTCTCGTCTCCTGGCAGCAG CTCCCCTGGTCACAGTAAAAGGAGCCCTCAGATGCCTGAGGAACCACTGGATATGAATGTGAAAATATAT CCAAGATATCCACTAGAAAGCAAAAGTAACTGTTTGCAGACTAGCCGAAGCCATTCTCGATGTGAAACCA AGAGCTCCAGTCCTACTCCGAGAGCTCCATCTGCAGAATTCTATCATGGGCAATCACTAGGGAAGCCTCT GGCCCTTCAGGCTGCCCTCCATGGACAGGTGTCTATTGGTAATGGGAAGAATCAGCCTAACTCCAGGGTT TCTAGTTCCAATGGCCTGGAGGGCAACTGGTCTGGGAACATCACCCAGAAAGTTAATTCAAGTGAGGTGT GCTATGACCAGGAGTCCATGCTCAGCTCCCACTTACCCAGTCCAGGCAACATTAGAAAATCTAGCATGGA ACATTTCAGCAGGTCCTTCAAGGAGGCCACCAATACATGGGTGAAGCCTACTGAGGACCTCCAGTACTGT GTTAAGCCAACTAAGAATGTGAGCTCTAAGGAGCAGTTGTGGGGTAGACAGCTTCTCAGGCGTCCCACAG GAAGAGCTTCATATCAGGAAACTGATGGCTATTGCCCTGATTTGGAGCCCAGTGATTCAGAAGCAGAAGG GGAGGGGAGTAAAGAAACACCAAGGGTAAAGAGAGAGAGTTCTGACAGAGAAAATCCTTCCCATGATTCT GCTCGGGAGTGCCATGGGAAAACCAAGACACACCCTCATTCCCACAGCTCAATGCAAAGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289684
Insert Size	2163 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289684.1, NP_001276613.1
RefSeq Size	5009 bp
RefSeq ORF	2163 bp
Locus ID	382207
Gene Summary	Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains an alternate exon in the 5' region and initiates translation at a downstream start codon, compared to variant 1. This results in a protein (isoform 2) that has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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