Clcn4 (NM_001302387) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Clc4-2; Clcn4-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228753 representing NM_001302387 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTTCCTCGAGGAGCCCTTCCCTGACGTGGGGACCTACGAGGACTTCCACACCATAGACTGGCTGA GGGAAAAGTCCCGGGATACCGACAGACATAGGAAGATCACCAGCAAAAGTAAGGAGTCTATTTGGGAGTT CATCAAGAGCCTGCTGGACGCGTGGTCGGGATGGGTGGTGATGCTACTCATTGGGCTGCTGGCAGGTACC TTGGCTGGAGTCATCGATCTCGCTGTGGATTGGATGACGGACCTCAAGGAGGGGGTCTGTCTGTCCGCAT TCTGGTACAGCCATGAACAGTGCTGTTGGACCTCCAACGAGACCACTTTTGAGGACAGGGACAAGTGTCC CCTGTGGCAGAAGTGGTCAGAGCTTCTTCTGAGCCAGTCAGAGGGCGCCAGCGCTTACATTCTGAATTAC TTAATGTACATTCTATGGGCGTTGCTGTTTGCATTTCTGGCTGTCTCCCTGGTACGTGTGTTCGCACCGT ATGCCTGTGGCTCTGGCATACCCGAGATAAAGACTATTTTGAGTGGCTTTATCATCAGGGGATACTTGGG GAAATGGACTCTTCTAATCAAGACTGTCACCCTCGTGCTCGTCGTATCCTCTGGCCTGAGCCTTGGCAAA GAGGGCCCACTGGTGCATGTGGCATGTTGCTGTGGCAACTTCTTCAGCAGCCTTTTCTCCAAGTATAGCA AGAATGAAGGCAAGAGGCGTGAGGTGCTTTCAGCTGCAGCTGCTGCTGGTGTCTCTGTGGCCTTTGGTGC TCCGATAGGAGGTGTGCTCTTCAGTCTAGAGGAGGTCAGTTACTACTTTCCCTTGAAAACCTTGTGGAGG TCATTCTTTGCAGCCCTGGTGGCTGCCTTCACACTGCGCTCCATCAACCCCTTTGGAAATAGCCGCCTGG TTCTCTTTTATGTGGAGTATCATACACCCTGGAGGCGTAAGACCACCAGGCTGGGCAGGTACCCAGTGTT GGAGGTTATTGCGGTGACAGCCGTCACCGCCATCGTGGCCTACCCCAATCCCTACACTCGCCAGAGCACC AGTGAGCTCATCTCTGAGCTCTTCAACGATTGTGGGGCTCTCGAGTCTTCTCAGCTCTGTGACTACATCA ACGACCCCAACATGACTCGGCCTGTGGATGACATTCCGGACCGGCCGGCTGGGGTTGGAGTTTACACAGC CATGTGGCAGCTGGCCTTGGCACTGATCTTCAAAATAGTCATTACTATATTTACCTTTGGCATGAAGATT CCCTCAGGTCTCTTCATCCCCAGTATGGCTGTCGGAGCCATGGCAGGCCGGATGGTGGGAATCGGTGTGG AGCAGCTGGCCTACCATCACCATGACTGGATCATCTTCAGGAACTGGTGCAGGCCTGGAGCGGACTGTGT CACACCAGGGCTTTATGCGATGGTGGGAGCTGCAGCCTGTCTAGGTGGGGTGACTAGGATGACAGTGTCT CTAGTGGTCATTATGTTTGAACTGACTGGAGGTCTGGAGTATATTGTACCCCTGATGGCAGCTGCTGTCA CCAGCAAGTGGGTGGCTGATGCCTTTGGGAAAGAAGGGATTTATGAAGCCCACATCCATCTGAATGGGTA CCCATTTCTTGATGTGAAGGATGAGTTCACCCACCGTACGCTGGCCACTGATGTGATGCGGCCCCGGAGG GGAGAACCGCCATTATCGGTACTAACCCAGGACAGCATGACTGTGGAGGACGTGGAGACTCTCATCAAGG AGACAGACTACAACGGCTTTCCTGTGCTCGTCTCCAGAGACTCGGAGCGTCTCATCGGGTTTGCCCAGAG GCGGGAGCTAATCTTGGCTATAAAAAATGCCAGGCAGAGGCAAGAGGGCATTGTGAGCAATTCCATCATG TACTTCACAGAGGAGCCTCCTGAGCTGCCTGCCAACAGCCCACATCCACTGAAGCTGAGGCGCATTTTGA ACCTGAGCCCTTTCACGGTCACAGATCACACCCCCATGGAGACGGTGGTGGACATTTTCCGGAAACTGGG GCTCCGACAATGCCTGGTGACACGGAGTGGGAGACTTCTTGGGATCATCACAAAAAAGGATGTTCTGAGA CACATGGCCCAGATGGCAAACCAGGACCCTGAATCCATCATGTTTAATTAG ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-NotI
ACCN	NM_001302387
Insert Size	2151 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302387.1, NP_001289316.1
RefSeq Size	4231 bp
RefSeq ORF	2151 bp
Locus ID	12727
Gene Summary	Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate in-frame splice site in the central coding region, compared to variant 1. It encodes isoform 3 which is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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