Dnm1l (NM_001276340) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6330417M19Rik; AI450666; Dlp1; Dnm; Dnmlp1; Dr; Drp1; pyt; python
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228752 representing NM_001276340 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCGCTGATCCCGGTCATCAATAAGCTGCAGGACGTCTTCAACACAGTGGGTGCGGACATCATCC AGCTGCCTCAGATCGTCGTAGTGGGAACGCAGAGCAGTGGGAAGAGCTCAGTGCTGGAAAGCCTAGTGGG CAGGGACCTTCTTCCCAGAGGAACTGGTGTGGTCACCCGGAGACCTCTCATTCTGCAGCTAGTCCACGTT TCACCAGAAGATAAAAGAAAAACAACAGGAGAAGAAAATGGTAAATTTCAGAGCTGGAGAGTTGAAGCAG AAGAATGGGGTAAATTTCTTCACACCAAAAACAAGCTTTACACAGATTTTGATGAAATTCGACAAGAAAT TGAAAATGAAACTGAAAGAATTTCAGGAAATAATAAGGGGGTAAGCCCTGAGCCAATCCATCTCAAGGTT TTCTCGCCCAACGTTGTCAACCTGACACTTGTGGATTTACCGGGAATGACCAAAGTACCTGTAGGCGATC AGCCCAAGGACATCGAGCTTCAGATCAGAGAACTTATTCTTCGGTTCATCAGTAATCCCAATTCCATTAT CCTCGCCGTCACTGCTGCAAATACAGATATGGCAACATCAGAAGCACTCAAGATTTCCAGAGAGGTAGAT CCAGATGGGCGCAGAACTCTAGCTGTAATCACTAAACTTGATCTCATGGATGCGGGTACTGATGCCATGG ATGTATTGATGGGAAGGGTTATTCCAGTCAAGCTTGGAATAATTGGAGTAGTTAACAGAAGCCAACTGGA TATTAACAATAAGAAGAGTGTAACTGATTCAATCCGTGATGAGTATGCTTTTCTTCAAAAGAAGTACCCA TCTCTGGCCAACAGAAATGGAACAAAGTATCTTGCTAGGACCCTGAATAGGTTACTTATGCATCATATCA GAGATTGTTTACCAGAGCTGAAAACAAGAATAAATGTCTTAGCTGCTCAGTATCAGTCTCTTCTAAATAG CTATGGTGAACCGGTGGATGATAAAAGTGCTACTTTACTCCAGCTTATTACCAAATTTGCCACAGAGTAT TGTAACACGATTGAAGGAACCGCAAAGTACATTGAAACTTCTGAGCTATGCGGTGGTGCTAGGATTTGTT ATATTTTCCATGAGACTTTCGGGCGAACCTTAGAATCTGTGGACCCACTAGGTGGCCTTAACACTATTGA CATCCTGACTGCCATCAGAAATGCTACTGGCCCCCGTCCTGCTTTATTTGTGCCTGAAGTTTCATTTGAG TTACTGGTCAAACGTCAGATTAAGCGTCTAGAAGAGCCCAGCCTACGGTGTGTGGAGCTGGTCCATGAGG AGATGCAGAGGATCATTCAGCATTGTAGCAATTACAGCACACAGGAATTGTTACGGTTCCCTAAACTTCA CGATGCCATAGTTGAAGTAGTGACCTGTCTTCTTCGTAAAAGGTTGCCCGTGACAAATGAAATGGTGCAT AACTTAGTGGCAATTGAGCTAGCGTATATCAACACAAAACACCCCGACTTTGCTGATGCCTGTGGGCTAA TGAACAATAATATAGAGGAACAAAGAAGAAACAGGCTAGCAAGAGAGCTGCCTTCAGCTGGATCACGGGA CAAGTTAATTCAGGACAACAGAAGAGAAACTAAAAATGTCCCATCTGCAGGTGGTGGGATTGGAGACGGT GGTCAGGAACCAACAACAGGCAACTGGAGAGGAATGCTGAAAACTTCAAAAGCTGAAGAATTACTTGCTG AAGAAAAATCAAAACCAATTCCAATTATGCCAGCAAGTCCACAGAAAGGCCATGCTGTCAATTTGCTAGA TGTGCCAGTTCCAGTTGCAAGAAAACTGTCTGCCCGAGAACAGCGAGATTGTGAGGTTATTGAAAGACTT ATCAAATCATATTTTCTAATTGTCAGAAAGAATATTCAAGACAGTGTCCCAAAGGCAGTAATGCATTTTT TGGTTAATCATGTGAAAGATACTCTTCAGAGTGAACTGGTAGGGCAGCTGTATAAGTCATCCTTATTAGA TGACCTTCTGACTGAATCCGAGGACATGGCCCAACGAAGAAAAGAAGCAGCGGATATGCTGAAGGCATTA CAAGGAGCCAGTCAAATTATTGCTGAAATCCGAGAGACTCATCTTTGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276340
Insert Size	2151 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276340.1, NP_001263269.1
RefSeq Size	4085 bp
RefSeq ORF	2151 bp
Locus ID	74006
UniProt ID	Q8K1M6
Gene Summary	This gene encodes a member of the dynamin family. The encoded protein is localized to the cytoplasm and mitochondrial membrane, is involved in mitochondrial and peroxisomal division, and is essential for mitochondrial fission. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 2. [provided by RefSeq, Feb 2013] Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, compared to variant 6. The encoded isoform (c) is shorter than isoform e. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.

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