Tdrd3 (NM_001253755) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4732418C03Rik; 6720468N18
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228751 representing NM_001253755 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGGTGTCCGGCGCCGCGTTGTCCCAGGCGGGTTGGTACCTTTCAGATGAAGGCGTTGAAGCCT GCACAAGCTCTCCTGGGAAAGGCAGCATAAATGACATCATCCTGATTGCTCTCAATACAGATTTGAGAAC AATTGGCAAGAAGTTCCTTCCCAGTGACATCAACGGTGGAAAGGTAGAGAAGCTTGAAGGTCCATGTGTC TTACAAATTCAGAAGGTCCGCAATGTTGCTGCGCCAAAGGATAACGAGGAATCGCAAGCTGCGCCAAGGA TGCTCCGCGTGCAGATGACGGATGGGCATACAAGTTGTACGGCAGTAGAATTCAGTTATATTTCAAAAAT AAGCCTGAACACACCACCTGGCACAAAAGTGAAGCTCTCTGGCACTGTGGACATAAAGAACGGATTCCTG CTCTTAAGTGACTCTAACACCACAGTTCTTGGTGGTGAAGTGGAACACCTAATTGACAAGTGGGCTTTAC AGAGAAGCTTATTAAAGCATAACAGAAGCAATATTGGAGCTGAAGGTGGCCCACCTCCTTTTCTACCTTT TGGGCAGAAGTGTGCATCTAACGTCCAAGTGGATAGCAGAGAGCTTGATCGAAGAAAGACATTACAAGTT TCCCTGCCTGCCAAGCCTGCAAATGATAATGATGAATTTGAAAAGCAAAGAACTGCTGCCATTGCAGAGG TTGCAAAGAGCAAAGAGACCAAGACATTTGGAGGAGGTGGAGGTGGTGCCAGAAGTAATCTCAATATTGG TGCTGCTGGTCACCGAAATAGGGAGGTTTTACAGAAGGAAAAGGCTTCCAAGTCAGAGAGCAAGAACGAA GGTGTCTATAGAGAATTGGTTGATGAGAAAGCCCTGAAACACATAACAGAAATGGGCTTCAGTAAGGAGG CCTCAAGGCAAGCGCTGATGGATAATGCCAACAACTTAGAAGCAGCACTGAATGTCCTTCTGAACAGCAG TAAACAGAAGCCTGCTGTGGGCCCTCCTGCTAGAGGAAGAGGGAAAGGCAGGGGTCGAGGACGATCAGAA GATGAGGAAGACCTGGGAACCGCAAGACCGTCAGCACCAAGCACGTTATTTGATTTCTTGGAGTCTAAAA TGGGAACTCTGAATGTGGAAGAACCTAAGTCACAGCCACAGCACCTTCATCAAGGACAGCACAGAGGATG GAATGCCGAGCAAAATGGGATGAAGGACGGCACTCAGTCAAGACATCTTCCTCGAAACGATACCAGGCAG CCAAGAAATGAGAGGCCACCTCGCTTTCAAAAAGACACCCCAACTTCCAAGTCAACTGTAGAAAACAGCG TATTATCTAGAAATAGAGGCTCCGAAAGGCCGAGTAGTTCCTCAGGTTCTGATGTATGGGCAGAAGAGAG GATCAAGTGTGACAGGCCATATTCTAGGTATGACAGAACTAAAGACGCTTCACATCCTCTAGGCCTTCAG CACAACGATGGTGCTTTTAAAAAGAGAGAGAACTCTATGCAAAACAGACCAGGAAGAGGCCCTTTGTATG CAGAGGCCAAAGAAAACCCACTTCCTCCAGAATTCGTAGATTACAATAATCAAAGACGTGGGAGAAGAGA AAACCAAACAGGTCATCCTGATCACTGTTATGAGAGGAAACCACGGACAATGAATAGTGAAGCTGTTAGT GGTCTAAAAATTGAGAAACATTTTAGTGTAAACACTGATTACCCGAGGCCTGTGCAGAGCAATAGTCTTG GTGTTCCAAATGGAGAGACAGCTCCGCCTCTGAAAGGAAGGCGCGTAGGACCGATTAAGTCAGCAGGACC TGTCACAGCTGTGCCCTACGATGATAAAATATTTTATAATAGTGGGCCCAAAAGAAGGTCTGGGCCAATT AAGCCTGAAAAGGTGATAGAATCATCTATTCCTGTGGAGTATGCAAAAGTGTGGAAACCTGGCGATGAAT GTTTTGCACTTTATTGGGAAGACAACAAGTTTTACCGAGCAGAAGTTGAAGCCTTGCATTCTTCGGGTAT GACAGCGGTCGTTAAGTTCACGGACTACGGCAACTATGAAGAGGTGCTACTGAGCAATATCAAGCCTGTT CAGACAGAGGCATGGGTACGTGATCCAAATTCTGTACAAAGGCTACTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253755
Insert Size	2151 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253755.1, NP_001240684.1
RefSeq Size	5762 bp
RefSeq ORF	2151 bp
Locus ID	219249
UniProt ID	Q91W18
Gene Summary	Scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. In nucleus, acts as a coactivator: recognizes and binds asymmetric dimethylation on the core histone tails associated with transcriptional activation (H3R17me2a and H4R3me2a) and recruits proteins at these arginine-methylated loci. In cytoplasm, may play a role in the assembly and/or disassembly of mRNA stress granules and in the regulation of translation of target mRNAs by binding Arg/Gly-rich motifs (GAR) in dimethylarginine-containing proteins (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks two exons and its transcription extends past a splice site that is used in variant 1, resulting in a novel 3' coding region and 3' UTR compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1.

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