Usp44 (NM_001206851) Mouse Untagged Clone

CAT#: MC228745

Usp44 (untagged) - Mouse ubiquitin specific peptidase 44 (Usp44), transcript variant 1



  "NM_001206851" in other vectors (1)

CNY 6,840.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms E430004F17Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228745 representing NM_001206851
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATAGGTGCAAGCACGTTGAGCAGTTGCAGCTTGCTCAAGGCCATTCCATCTTGGACCCTCAGAAAT
GGTACTGTATGGTCTGCAACACAACCGAGTCCATTTGGGCTTGCCTCAGCTGTTCCCACGTCGCCTGTGG
GAAGTACATCCAAGAGCACGCACTGAAGCACTTTCAAGAAAGCAGCCATCCCGTTGCCTTCGAGGTGAAC
GACATGTACGCTTTTTGTTATCTTTGTAACGATTATGTTCTAAACGATAACGCAGCCGGAGACCTGAAGT
CACTACGGAGTACACTAAGTACAATCAAAAGTAAAAAGTACCCCTGTGTGGTTCCGAGTGACTCGGTTTT
ACATCCTGTGGATGCACAGGACCGTGTTTACTCCTTACTCGACGGCACCCAATCTCTGCCCGGAAACGAG
GATCCAACGTGCGCCGCCCTCTGGCACAGGAGACGGGTGCTCATGGGGAAAGCCTTTCGAACTTGGTTCG
AACAGTCAGCAATTGGAAGGAAAGGGCAAGAGCCAACTCAGGAGAGAATGGTAGCAAAACGAGAGGCGAA
GAGAAGGCAGCAGCAGGAGTTGGAGCAGCAGATGAAAGCCGAGCTGGAAAGCACACCTCCGCGGAAGAGT
TTACGCTTACAAGGCTCCTCGGAGGAGGCGGCCACAATCGAAATCGTGCCAGTGCGGGCACCGCCCCCAC
CTCCCGCATCCCCAGCAAAGGACAAAGCCGCGCTACCTACTTCGGAAGACAGGACTTTTAAAAAAGTAAG
TGACTCCTTAATCAAACGAAGGCCCATGGTAACTCCTGGTGTAACCGGACTGAGAAACTTAGGAAATACT
TGCTATATGAATTCTGTTCTTCAAGTGTTGAGTCATTTACTCATTTTTCGACAATGTTTTTTAAAGCTTG
ACCTGAACCAGTGGCTGGCTGTGGCTGCCAGCGATAAGGCCCGATCCTATAAGCACTCAGCCGTCACGGA
GGCGGCGGCACAGCAGATGAACGAAGGGCAAGAGAAAGAGAAAGGCTTCGTATGCTCCAGACATTCGGGT
TTATCCTCAGGCCTGAGCGGAGGGGCCTCAAAAGGTCGGAACATGGAGCTTATTCAGCCAAGGGAGCCCA
GTTCCCCGTACAGTTCTCTCTGCCATGAATTGCATATCCTGTTCCAAGTCATGTGGTCTGGAGAGTGGGC
CTTGGTCTCACCGTTTGCCATGCTTCACTCCGTGTGGAGGCTGATCCCTGCTTTCCGCGGTTACGCCCAG
CAGGATGCTCAGGAATTTCTTTGTGAACTTCTGGATAAGATACAACGTGAACTAGAGACAACCGGAACCA
AGTTACCAGCCCTCATCCCCACTTCCCAAAGGAGACTTATTGAGCAAGTTCTGAATGTGGTGAATAACAT
TTTTCACGGACAATTTCTTAGTCAGGTTACATGTCTTGCTTGTGACAACAAATCAGATACCATAGAATCC
TTCTGGGACTTGTCCCTGGAGTTTCCAGAGAGATACCAGTGCAGTGGGAAAGATGCTGCCTCCCAGCCGT
GCCTGGTTACTGACATGTTGGACAAATTTACAGAAACAGAAGCTTTAGAAGGGAAAATCTACATGTGTGA
CCATTGTAACTCAAAGCGTAGAAAGTTTTCGTCAAAATCAGTTGTATTCACAGAAGCCCAGAAGCAGCTT
ATGATATGCCATCTACCTCAGGTTCTCAGACTGCACCTCAAACGGTTCAGATGGTCAGGACGTAATAACC
GAGAGAAGATTGGTGTTCATGTTGTCTTTGAGGAAACCTTAAACATGGAGCCTTATTGCTGCAGGGAGAC
CCTGAACGCTCTCAGACCAGAGTGCTTTCTCTATAATTTATCTGCTGTCGTAATTCACCATGGAAAAGGA
TTTGGCTCAGGACACTACACTGCCTACTGCTACAATTCCGAAGGAGGGTTCTGGGTCCACTGCAACGATT
CCAAGCTGAGCATGTGCACGATGGAAGAAGTACGCAAGGCCCAAGCTTATATCTTATTTTATACTCAGCG
AGTTACTGAGAATGGACATTCGAAACTCTTGCCTCCCGAGCTCCTGTCCAATAGCCAGCACCCCAGTAAG
GAGACTGATGCCTCTTCTAATGAAGTCCTTAGCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001206851
Insert Size 2136 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001206851.1, NP_001193780.1
RefSeq Size 2533 bp
RefSeq ORF 2136 bp
Locus ID 327799
UniProt ID Q8C2S0
Gene Summary Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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