Usp44 (NM_001206851) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	E430004F17Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228745 representing NM_001206851 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAGGTGCAAGCACGTTGAGCAGTTGCAGCTTGCTCAAGGCCATTCCATCTTGGACCCTCAGAAAT GGTACTGTATGGTCTGCAACACAACCGAGTCCATTTGGGCTTGCCTCAGCTGTTCCCACGTCGCCTGTGG GAAGTACATCCAAGAGCACGCACTGAAGCACTTTCAAGAAAGCAGCCATCCCGTTGCCTTCGAGGTGAAC GACATGTACGCTTTTTGTTATCTTTGTAACGATTATGTTCTAAACGATAACGCAGCCGGAGACCTGAAGT CACTACGGAGTACACTAAGTACAATCAAAAGTAAAAAGTACCCCTGTGTGGTTCCGAGTGACTCGGTTTT ACATCCTGTGGATGCACAGGACCGTGTTTACTCCTTACTCGACGGCACCCAATCTCTGCCCGGAAACGAG GATCCAACGTGCGCCGCCCTCTGGCACAGGAGACGGGTGCTCATGGGGAAAGCCTTTCGAACTTGGTTCG AACAGTCAGCAATTGGAAGGAAAGGGCAAGAGCCAACTCAGGAGAGAATGGTAGCAAAACGAGAGGCGAA GAGAAGGCAGCAGCAGGAGTTGGAGCAGCAGATGAAAGCCGAGCTGGAAAGCACACCTCCGCGGAAGAGT TTACGCTTACAAGGCTCCTCGGAGGAGGCGGCCACAATCGAAATCGTGCCAGTGCGGGCACCGCCCCCAC CTCCCGCATCCCCAGCAAAGGACAAAGCCGCGCTACCTACTTCGGAAGACAGGACTTTTAAAAAAGTAAG TGACTCCTTAATCAAACGAAGGCCCATGGTAACTCCTGGTGTAACCGGACTGAGAAACTTAGGAAATACT TGCTATATGAATTCTGTTCTTCAAGTGTTGAGTCATTTACTCATTTTTCGACAATGTTTTTTAAAGCTTG ACCTGAACCAGTGGCTGGCTGTGGCTGCCAGCGATAAGGCCCGATCCTATAAGCACTCAGCCGTCACGGA GGCGGCGGCACAGCAGATGAACGAAGGGCAAGAGAAAGAGAAAGGCTTCGTATGCTCCAGACATTCGGGT TTATCCTCAGGCCTGAGCGGAGGGGCCTCAAAAGGTCGGAACATGGAGCTTATTCAGCCAAGGGAGCCCA GTTCCCCGTACAGTTCTCTCTGCCATGAATTGCATATCCTGTTCCAAGTCATGTGGTCTGGAGAGTGGGC CTTGGTCTCACCGTTTGCCATGCTTCACTCCGTGTGGAGGCTGATCCCTGCTTTCCGCGGTTACGCCCAG CAGGATGCTCAGGAATTTCTTTGTGAACTTCTGGATAAGATACAACGTGAACTAGAGACAACCGGAACCA AGTTACCAGCCCTCATCCCCACTTCCCAAAGGAGACTTATTGAGCAAGTTCTGAATGTGGTGAATAACAT TTTTCACGGACAATTTCTTAGTCAGGTTACATGTCTTGCTTGTGACAACAAATCAGATACCATAGAATCC TTCTGGGACTTGTCCCTGGAGTTTCCAGAGAGATACCAGTGCAGTGGGAAAGATGCTGCCTCCCAGCCGT GCCTGGTTACTGACATGTTGGACAAATTTACAGAAACAGAAGCTTTAGAAGGGAAAATCTACATGTGTGA CCATTGTAACTCAAAGCGTAGAAAGTTTTCGTCAAAATCAGTTGTATTCACAGAAGCCCAGAAGCAGCTT ATGATATGCCATCTACCTCAGGTTCTCAGACTGCACCTCAAACGGTTCAGATGGTCAGGACGTAATAACC GAGAGAAGATTGGTGTTCATGTTGTCTTTGAGGAAACCTTAAACATGGAGCCTTATTGCTGCAGGGAGAC CCTGAACGCTCTCAGACCAGAGTGCTTTCTCTATAATTTATCTGCTGTCGTAATTCACCATGGAAAAGGA TTTGGCTCAGGACACTACACTGCCTACTGCTACAATTCCGAAGGAGGGTTCTGGGTCCACTGCAACGATT CCAAGCTGAGCATGTGCACGATGGAAGAAGTACGCAAGGCCCAAGCTTATATCTTATTTTATACTCAGCG AGTTACTGAGAATGGACATTCGAAACTCTTGCCTCCCGAGCTCCTGTCCAATAGCCAGCACCCCAGTAAG GAGACTGATGCCTCTTCTAATGAAGTCCTTAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001206851
Insert Size	2136 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001206851.1, NP_001193780.1
RefSeq Size	2533 bp
RefSeq ORF	2136 bp
Locus ID	327799
UniProt ID	Q8C2S0
Gene Summary	Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination.[UniProtKB/Swiss-Prot Function]

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