Add3 (NM_001277100) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI463285; R75380
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228732 representing NM_001277100 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCCGACACCAGCCCAGCTGTGGTTACCACGCCTCCTCCTCCCAGCATGCCTCACAAAGAGAGGT ATTTTGACCGAATCAATGAAAGTGATCCGGAATACCTTCGAGAGAGGAACATGTCTCCTGATCTACGACA GGACTTCAATATGATGGAGCAGAGGAAGCGAGTCACTCAGATCCTGCAGAGCCCTGCCTTTCGGGAAGAC CTGGAATGCCTCATCCAAGAGCAGATGAAGAAAGGCCACAACCCAAGCGGCTTATTAGCGTTACAGCAGA TTGCGGATTACATCGTGACCAGCTCCTTCTCGGGCTTCTCCTCGCCCTCCCTGAGTCTTGGCATGGTCAC ACCTATTAATGACCTTCCTGGTGCAGATACATCCTCGTATGTGAAGGGAGAAAAGCTTACTCGCTGCAAA CTTGCCAGCCTCTACAGGCTTGCAGACTTGTTTGGGTGGGCGCACCTGGCAAATACGTACATCTCGGTAC GAATAAGTAAGGAGCAAGACCACATTATAATAATTCCCAGAGGCCTCTCTTTTTCTGAAGCGACAGCCTC CACTTTGGTGAAAGTCAACATAATCGGAGAAGTGGTGGACCAGGGGAGCACTGATCTGAAAATCGACCAC ACGGGCTTCAGCCCGCATGCTGCCATCTATTCAACACGCCCCGATGTGAAGTGTGTGATCCACATCCACA CCCTTGCCACAGCAGCGGTGTCCTCCATGAAGTGTGGGATCCTTCCAATTTCCCAAGAGTCTCTCATCCT GGGAGATGTCGCCTACTATGACTACCAGGGGTCGCTTGATGAGGAGGAAGAAAGAATTGAACTTCAGAAA GTGCTGGGTCCAAGCTGTAAGGTGCTCGTCCTCAGGAACCATGGCATGGTGGCTCTTGGCGAGACCCTCG AGGAGGCTTTCCATTACATCTTTAATGTACAGATGGCCTGTGAGATCCAGGTGCAGGCAGTAGCAGGGGC AGGCGGAGTAGACAATCTGCTCGTACTGGATCTTCAGAAATATAAAGCTTTCACCCACGGTGTCGCCATG TCTGGAGGAGGCGGTGTAAACATGGCTTCCCATCAGAAGTGGAAGGTTGGCGAGATTGAGTTTGAAGGGC TGATGAGGACCCTGGACAACTTGGGCTACAGAACAGGCTATGCGTACAGGCATCCTCTGGTCCGAGAGAA GCCTAGGCACAAGAGTGACGTGGAGATCCCTGCAACCGTGACTGCCTTCTCCTTTGAAGATGACAGTGCT CCACTCTCTCCCCTCAAGTTCATGGCACAGAGACAGCAGCGTGAAAAAACAAGATGGCTGAATTCACCAA ATACTTACATGAAGGTGAATGTGCCGGAGGAGTCTCGGAACGGGGAAACTAGTCCCAGGACCAAAATCAC GTGGATGAAAGCAGAAGACTCCTCTAAAGTCAGTAGTGGGACACCGATCAAAATTGAAGACCCCAATCAG TTTGTTCCTTTAAACACAAACCCGACGGAGGTGCTAGAAAAGAGAAATAAGATCCGGGAACAGAATCGCT ATGATCTGAAGACAGCAGGGCCGCAGTCTCAGCTGCTGGCGGGGATCGTTGTGGACAAGCCGCCTTCGAC CATGCAGTTTGACGATGACGATCAGGGCCCACCAGCTCCTCCTAACCCATTCAGCCACCTCCTGGAAGGA GAACTGGAAGAGTATACGAAGACAATCGAGAGAAAGCAGCAAGGCCTGGACGACGCTGAGCAGGGGTCAC TCTCAGACGACGCTGCATCTGTTTCACAAATTCAGTCTCAAACTCAGTCACCGCAGAGTGTCCCTGAGAG ATTAGAAGAAAACCACGAGCTGTTTTCCAAGAGCTTCACCTCCATGGACGCGCCTGTCATGATCATGAAC GGCAAGGACGAGATGCATGATGTCGAAGACGAGCTTGCTCAGCGAGTCAGTAGGCTGACCACAAGCACGA CCATAGAGAACATCGAGATCACCATTAAGTCCCCAGAAAGAACTGAAGAGGTCCTGTCGCCTGATGGCTC ACCTTCAAAGTCGCCCTCCAAGAAAAAGAAGAAATTCCGCACGCCTTCTTTTCTCAAAAAGAACAAAAAA AAGGAGAAAGTTGAGGCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277100
Insert Size	2121 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277100.1, NP_001264029.1
RefSeq Size	4511 bp
RefSeq ORF	2121 bp
Locus ID	27360
UniProt ID	Q9QYB5
Gene Summary	Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Plays a role in actin filament capping. Binds to calmodulin.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) differs in the 5' UTR, compared to variant 1. Variants 1, 2, and 5 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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