Nsun7 (NM_001302833) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4921525L17Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228729 representing NM_001302833 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGACCCAACGAGTGAAAGGGACCTTTTTGACCAAGAAAACATGGAGGAGATCTCCCAACTCGCTT CCCTGGAGATGTCGGGGGATGTAGTCGCCAATACAAACTCTTCAACCGTCGTCCTAGAAAAACCCAGCTA CCCAGACTCCGTGTACGTTACGGCAGCGAACATTTTTCAGGGTATTCGGATCCAAAGGTCGCCAGACAAA GTCATAATAAATTACGGAAGTGAACCCCTGCAGCCCTCCTCCTCCCGCTCCGAGGATGAATCTTTCCAGC GCTTGTCCTATGAGTTGGCTTTCAGTACCCTGAAGTATCAAGATATTTTGGAAAGTATATTAATAGATAG CTATATCTTCTCAAGTACCACAATCGCAAGTCAGCTGAACAGTCTTATCATTGTGATGCTGTATGACTTC CAAGACAGAAAATTCCAGCCCCGCATCCTTTCCGAGAATGAAGAGACCATACCAGAAGTTCAAGAAGTCG AGAACCTTCTAAACGGCTTTAAGACAAAATTGGCTGCAGCCTTGGCGAGATGTCGAATCAAACACGACGC CCTCTCGATTTACCATATTCTGCCAGAGACAGTTAGGAAACAGGAACAAAGGGCCTCAACTTTGCCTCTT TATGCTTGGATAAATACCAGTAAAATAAGCCTTGAAGAAGTCTATAATAATTTGAGGAGGAAAGGTTACT CTAAAGTCAAGTCTATAACGAGTGTCAATGAGAAAGTCTATGCAGTAGACCAGCACTGCTTCAATGTCTT AATTTTCCCAGCTCATCTCAAAACAGACCTTTTAAACATAGATCTCATAAAAGACTATAAACTTATTTTC CAGGACAAATCTCGAAGTCTTGCCGTCCACTCTGTGAAGGCTTTGATAAACATCGATGATGATGTCCTAA TGGTCAACACGGGTTCGTGGTACACAGTTGCCCATATGTCCATCTTAACAAGTGGTCACACTTCGAAAAT CTTTGTATGTGGAATACAACAAGAAGAGAAGGATTTTAATGCGAGGAAACTTTTTACAAGAATGGGATGT CAAAATATTGAAATACTTCACGAAACATTTCTTAGCATTGAATCCAAAGATCACCGGTTACAGAATGTGA AGGTCATTCTGTTGCTACCTCGCTGTTCCAGCCTCGGTGTCAGCAACCCAGTGGAATTCATTCTAAATGA ACACGAAGATAAAAGTTTACTTCAAGATCTTTCTCAAGGTGGTCTACCGAAAGATAAACTCGAGACTTTG GTTCAGCAGCAGTTTGAACAGCTAACACACGCAATGAAATTTACCAAAGTTCAAGCAATTGTCTATTGCA CATGTTCTGTTTCTAAAGAGGAAAATGAAGACGTTGTTGAGAAAGCACTGGAATATCAAAGCTCAGGGGT TAAAATGCAACCTTACAGACTCAGTCCTCCTGTTCTCCCACTGTGCACCCTAAAAGAGATAGAACTGTCT ATGGATAGATTCTTCAGATTGGAACCATCTGATATGAACAATGGCTGTTTTCTTTCTATCTTAACAAGAG AGCGGGACCCATCTGAGACAGTGTCAGTGAAAGACGTTCTGGCCAGGGCAGCAGCCAAAGGTCTACTGGA AGGGGTTGAGGTTGGGAAGACGCTCAAAAGGGACAAGAAGAGGAAAAAATCGAAAGCACTGCCATCCAGG GCTCCTCACCATGGGGACCCACTTCGCGACCATTTGGCAGTGGATGGTAACGACACCAGCAACGTCCAAA TGAAAATCTCCGAGCTCTTGCACCGAGAAAGCAAGATAAGTACTTCAACAAAAATGTCCGCCCCAGCAAA GACTGTGTCTCAAGCCGGCACTTCCTCCCAAGTCAGAAAGCCCAGCAAGCCTTTATCGACCCCACTGGTG AGGAATTTTTCCAGGCCGGTAGAAAGGCCAACGAACTTTGTGAGAGCGCGGCCAGAAGGCAAAGTGATTC CTTTGAAACCAATTGAAATCGTTCTGCCGCCAGTGATATTCCCATTGAGTTCCCAGGGGCCCAGGGTTCA GATGCCAGCCACCCATTTTTATTACCGCTTCATTGGATCAAAGGACTCTTCTAGGGAGGACAGGATCAAT AATTTCCTGATAAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302833
Insert Size	2118 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302833.1, NP_001289762.1
RefSeq Size	2772 bp
RefSeq ORF	2118 bp
Locus ID	70918
UniProt ID	Q14AW5
Gene Summary	This gene encodes a member of the NOL1/NOP2/sun domain RNA methyltransferase family. Mice with a mutation in this gene exhibit male sterility due to impaired sperm motility. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2014] Transcript Variant: This variant (1) encodes the longest isoform (1).

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