Gpsm1 (NM_001199147) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1810037C22Rik; Ags3; AW107933
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228727 representing NM_001199147 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGCTTCTGCTATGGAGGGCCAGCCTCTGGCCCTGAGCTTGGCAGAGAAGGCTGTGTGCAAGGTGG TATATGGTGCCCCTCGCCCTCGCCCATTGCTGCTGCCCGTGGGCCTGGAGCTGTGGCTGTATGTACAGAA GATGCGTAACCTCCAGAGAAAGAGGATGGAGGCCTCCTGTCTAGAGCTGGCCCTGGAAGGGGAGCGGCTG TGCAAGGCGGGGGACTTCAAGGCAGGTGTGGCTTTCTTCGAGGCTGCTGTGCAGGTGGGCACTGAGGACC TGAAGACACTGAGTGCCATCTACAGCCAGCTGGGCAATGCCTACTTCTACCTGAAGGAGTATGCCCGGGC CCTGCAGTTCCACAAACATGACTTGCTGCTGGCACGGACCATTGGTGACCGCATGGGGGAGGCCAAGGCT AGTGGGAACCTGGGCAACACACTCAAGGTCCTAGGCCGATTCGATGAGGCAATCGTCTGCTGCCAACGAC ACTTGGACATTGCCCAGGAGCAGGGGGACAAGGTTGGGGAGGCGAGAGCACTCTACAACATTGGAAATGT GTACCACGCCAAGGGCAAACAGCTTTCCTGGAATGCTGCACAGGACCCCGGGCACCTGCCACCTGATGTC CGCGAGACACTGCACAGGGCCTCTGAGTTTTATGAGAGGAACCTGTCTTTGGTGAAGGAACTAGGCGACC GGGCGGCCCAGGGCAGGGCCTATGGCAACCTGGGTAACACCCACTACCTACTGGGAAACTTCACGGAGGC CACAACCTTCCACAAAGAGCGCCTGGCCATCGCCAAGGAGTTTGGGGACAAGGCAGCTGAGCGGAGGGCC TACAGCAACCTGGGCAATGCTCACATCTTCTTGGGGCGCTTTGATGTGGCTGCTGAACATTACAAGAAGA CGCTGCAGCTGTCTCGGCAGCTCCGGGACCAGGCAGTGGAGGCTCAGGCTTGCTACAGCCTGGGCAACAC CTACACACTGCTACAGGACTACGAGCGTGCTGCTGAGTACCACCTGCGGCACCTAGTCATTGCCCAGGAG CTGGCTGATAGGGTGGGAGAGGGCCGAGCATGCTGGAGCCTGGGGAACGCCTATGTGTCCATGGGGAGCC CTGCACAGGCCTTGACCTTTGCCAAGAAACATCTGCAGATCTCCCAGGAGATTGGAGACCGAAACGGAGA ACTGACAGCCCGCATGAATATTGCCCACCTGCAGCTGGCCCTGGGCCGGCTGACTAGCCCAGCAGCAGCG GAGAAGCCAGATCTGGCTGGCTATGAGGCACAAGGAGCAAGACCCAAACGGACACAGAGGCTGAGTGCCG AGACCTGGGACCTGCTGCGGCTCCCCCTGGACCGGGAGCAGAATGGAGAGACCCACCACACAGGGGACTG GAGGGGTCCAGGCAGGGACTCGCTTCCCCTCCCCATGAGGAGCAGGAAGTACCAGGAAGGTCCAGATGCC ATTGAGAGGAGACCCCGGGAGGGTAGTCACTCTCCACTGGACAGTGCTGATGTAAGGGTACAGGTGCCTC GTACGGGTATTCCCAGGGCCCCGTCTTCTGACGAGGAGTGTTTCTTTGACCTGCTGAGTAAGTTCCAGAG CAGTCGCATGGATGACCAGCGCTGTCCCCTGGAGGAAGGCCAGGCTGGGGCTGCTGAGGCCACAGCTGCC CCATCCGTGGAGGATAGAGCAGCTCAGTCCTCCGTGACAGCTTCACCACAGACAGAGGAGTTCTTTGACC TCATTGCCAGCTCCCAGAGCCGCCGGCTGGACGACCAGAGGGCTAGCGTAGGCAGCCTGCCTGGGCTACG CATCACCCTCAACAATGTGGGGCACCTCCGAGGCGACGGGGACGCCCAGGAGCCGGGGGATGAGTTTTTC AACATGCTTATCAAATACCAGTCCTCCAGGATTGATGACCAGCGCTGCCCACCCCCTGATGTGCTGCCCC GTGGCCCTACCATGCCTGATGAGGATTTCTTCAGCCTTATCCAGAGGGTGCAGGCTAAGCGGATGGATGA GCAGCGTGTGGACCTTGCTGGGAGTCCAGAGCAAGAGGCCAGTGGGCTGCCTGATCCCCAGCAGCAGTGT CCACCGGGTGCCAGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199147
Insert Size	2118 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199147.1, NP_001186076.1
RefSeq Size	3458 bp
RefSeq ORF	2118 bp
Locus ID	67839
UniProt ID	Q6IR34
Gene Summary	Guanine nucleotide dissociation inhibitor (GDI) which functions as a receptor-independent activator of heterotrimeric G-protein signaling. Keeps G(i/o) alpha subunit in its GDP-bound form thus uncoupling heterotrimeric G-proteins signaling from G protein-coupled receptors. Controls spindle orientation and asymmetric cell fate of cerebral cortical progenitors. May also be involved in macroautophagy in intestinal cells. May play a role in drug addiction.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (3).

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