Adarb2 (NM_001289530) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Adar3; RED2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228717 representing NM_001289530 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCTGTCCTGGGGAGTGGCAGAGGGTCTGGAGGGCTAAGCAGTCAACTCAAATGCAAGTCCAAGA GGAGGAGGAGGAGGAGGTCCAAGAGGAAAGACAAAGTCAGCATACTGTCAACCTTCCTTGCTCCTTTCAA GTACCTAAGTCCTGGCACCACAAACACAGAGGATGAAGATAACCTAAGTACCAGCAGTGCTGAAGTGAAG GAAAACCGAAATGTGAGCAACCTGGGGACCAGGCCCCTGCCCCCTGGAGACTGGGCCAGAGGCAGCACAC CCAGCGTTAAAAGGAAAAGGCCGCTGGAGGAAGGAAATGGGGGACATTTCTGCAAACTCCAGCTGATCTG GAAGAAGCTCTCCTGGTCTGTGACACCCAAGAATGCGCTGGTCCAGCTGCATGAGCTGAAGCCTGGTCTG CAGTACCGAATGGTGTCACAGACGGGCCCGGTGCACGCCCCAGTTTTCGCAGTGGCTGTAGAAGTGAACG GCCTCACATTTGAAGGCACGGGGCCCACTAAGAAGAAGGCCAAGATGCGGGCAGCAGAAATGGCCCTGAA GTCCTTTGTGCAGTTCCCCAATGCCTTCCAGGCCCACCTGGCCATGGGCAGTAGTACCAGCCCGTGCACT GACTTCACCTCTGACCAGGCTGACTTCCCAGACACGTTGTTCAAGGAGTTTGAACCATCCTCAAAAAACG AAGACTTCCCAGGCTGTCATCCCGTTGATACTGAGTTTCTGTCCTCTGCCTATCGGCGAGGACGGCTACT CTACCACACCCTGGACCTCATGGGCCAGGCTCTGCCTGACAGGTCCAGGCTGGCTCCGGGAGCCTTGGGT GAGAGGAACCCTGTAGTGGTACTGAATGAGTTACGCTCAGGTCTTCGGTATGTGTGCCTCTCCGAGACAG CCGAGAAGCCCCGAGTCAAGAGCTTTGTCATGGCTGTGTGCGTGGATGGGAGGACGTTCGAAGGCTCTGG ACGCAGCAAGAAGCTGGCCAAGGGCCAAGCAGCACAAGCTGCCTTGCAGGCCCTCTTCGATATCCGGCTG CCTGGTCACATACCCAGCAGGAGTAAAAGCAACCTCTTGCCACAGGATTTTGCAGACTCTGTGTCCCAGC TGGTCACACAGAAGTTCCGTGAACTGACAGTCGGCCTGACATCTGTGTATGCCCGCCACAAAACACTGGC AGGAATCGTCATGACCAAAGGCTTGGACACCAAACAAGCGCAGGTCATCGTTCTGTCCTCTGGGACCAAG TGTATCAGCGGTGAGCACATCAGTGACCAGGGGCTGGTTGTCAACGACTGTCATGCTGAGATCGTGGCGA GGCGGGCATTTCTTCACTTCCTCTACTCTCAGCTGGAGCTGCACTTGAGCAAGCATCAAGAGGACCCCGA GAGATCAATATTCATACGTTTAAAGGAAGGAGGTTACAGGCTGCGAGAAAACATCCTCTTCCATCTCTAC GTGAGCACATCCCCCTGTGGAGATGCCAGAGTCAACTCTCCATATGAAATCACCACAGACTTGAACAGCA GCAAACACATCGTCAGGAAGTTCCGAGGGCACCTGCGCACCAAGATTGAGTCTGGGGAAGGAACAGTCCC TGTGCGGGGCCCCAGTGCAGTCCAGACGTGGGATGGCATCCTATTGGGGGAGCAGCTAATCACCATGTCC TGCACAGACAAGATTGCCAGCTGGAATGTGCTGGGACTACAAGGCGCTCTCCTCTGTCACTTCATCGAGC CTGTGTACCTCCACAGCATCATTGTGGGCAGCCTGCACCACACGGGCCATCTTGCACGGGTCATGAGCCA CAGGATGGAGGGAATCGGCCAGCTGCCTGCCTCATACCGACAAAACAGGCCTCTCCTTAGTGGCGTGAGT CACGCAGAGGCCCGGCAGCCAGGAAAGTCTCCCCACTTCAGTGCCAACTGGGTCGTGGGCAGCGCCGACC TGGAGATCATTAATGCCACCACTGGGAAGAGGAGCTGTGGGGGCTCATCCCGGCTCTGCAAACACGTGTT TTCTGCTTGGTGGGCACGGCTGCATGCTAAGCACACGGATCCCCAGCCATGGAGACACACCATCCATGTA CTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289530
Insert Size	2106 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289530.1, NP_001276459.1
RefSeq Size	5628 bp
RefSeq ORF	2106 bp
Locus ID	94191
Gene Summary	Lacks editing activity. It prevents the binding of other ADAR enzymes to targets in vitro, and decreases the efficiency of these enzymes. Capable of binding to dsRNA but also to ssRNA (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1, which results in a frameshift and a protein (isoform 2) with a shorter and distinct C-terminus compared to isoform 1.

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