Cdc27 (NM_001285990) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI452358; APC3; BC023187
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228706 representing NM_001285990 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGACCTTGTCACTGAGTTTGGAGATTCAGCTTGCTTCACTCTTTCCTTGTTGGGACATGTGTATTG GTGAGAAGCCAGATCCTGACCAAACATTTAAATTAACATCTCTACAGAATTTTAGCAGTTGTCTTCCCAA CACTTGTACAACTCTAGTATCTAATCACAGTTTATCTCACAGACAGCCTGAGACAGTCCTTACAGAAACA CCCCAAGACACAATTGAATTAAACAGACTGAATTTAGAATCTTCCAATTCAAAGTACTCCTTGAATACAG ATTCCTCCGTGTCTTACATTGATTCAACTGTAATTTCACCAGATAACGTCCCCCTTGGACCTGGTACTGC CATATTATCTAAACAGGTTCAAAATAAACCAAAAACTGGTCGAAGTTTATTAGGAGGACCAACTGCTCTT AGCCCATTAACCCCAAGTTTTGGGATTTTGCCATTAGAAACCCCAAGTCCTGGAGATGGATCCTATTTAC AAAACTACACTAATACACCTTCTGTAATTGATGTGGCACCCACCGGAGCACCTACAAAAAAGACTTTTTG TGTTTTACAGTCTGTTGCAAGAATGGGCCAAACTGGAACAAAGTCTGTCTTCTCACAGAGTGGAAATAGT CGAGAGGTCACACCAGTACTTGTTGCACAAACACAAAGTTCTGGCCCACAAACAAGTACAACACCTCAGG TATTGAGCCCCACTATCACATCTCCCCCAAACGCATTGCCTCGGAGAAGTTCCCGCCTTTTCACTAGTGA CAGTTCTACAACCAAGGAGAATAGCAAAAAGTTAAAAATGAAGTTTCCACCTAAAATCCCTAACAGAAAA ACAAAAAGTAAAACTAATAAAGGAGGACTAACTCAGCCCAGCATAAACGATAGTCTGGAAATTACAAAAC TGGACTCCTCTATCATTTCAGAAGGGAAAATAACCACAGTCACACCTCAGATCCAGGCATTTAACCTACA AAAGGCAGCAGCAGAAGGTTTGATGAGCCTTCTTCGTGAAATGGGAAAAGGTTATTTAGCTTTGTGTTCA TACAACTGCAAAGAAGCTATCAATATTTTGAGCCATCTACCTTCTCATCACTACAGTACTGGTTGGGTCC TATGCCAGATTGGACGGGCTTATTTTGAACTTTCAGAATACATGCAGGCTGAAAGAATATTCTCAGAGGT TAGAAGGATTGAGAGTTTCAGAGTTGAAGGAATGGAGATCTACTCTACAACACTCTGGCATCTTCAGAAA GACGTTGCTCTTTCAGTTCTTTCAAAAGATTTAACAGACATGGATAAGAATTCACCAGAGGCCTGGTGTG CTGCAGGGAATTGTTTCAGTCTACAACGAGAGCATGATATAGCAATTAAATTCTTCCAAAGAGCTATCCA GGTCGATCCAAATTACGCTTACGCCTATACTCTATTAGGACATGAGTTTGTGTTAACTGAAGAACTAGAT AAAGCATTAGCATGTTTTCGAAATGCTATAAGAGTAAATCCCAGACATTACAATGCATGGTATGGTTTAG GAATGATTTATTACAAGCAAGAGAAGTTCAGCCTCGCAGAAATGCATTTCCAGAAAGCACTTGATATCAA CCCTCAGAGTTCAGTTTTACTTTGCCACATTGGAGTAGTTCAGCATGCACTAAAGAAGTCTGAGAAGGCT TTGGATACCCTAAACAAAGCCATTGTTATCGATCCCAAGAACCCTCTATGCAAATTTCACAGAGCCTCGG TTTTATTTGCAAATGAAAAATACAAGTCTGCTTTACAAGAACTCGAGGAACTGAAGCAGATTGTCCCCAA AGAGTCCCTCGTTTACTTCCTAATAGGAAAGGTTTACAAGAAGTTGGGTCAAACTCATCTCGCCTTGATG AATTTCTCTTGGGCTATGGATTTAGATCCTAAAGGAGCCAATAATCAGATTAAAGAGGCAATTGACAAGC GCTACCTTCCAGATGATGAGGAGCCAATAACCCAGGAGGAACAGATCATGGGGACAGATGAATCCCAGGA GAGCAGCATGACAGATGCAGATGACACACAACTTCATGCGGCGGAAAGTGACGAATTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285990
Insert Size	2091 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285990.1, NP_001272919.1
RefSeq Size	5703 bp
RefSeq ORF	2091 bp
Locus ID	217232
Gene Summary	Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region and uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 4), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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