Mybl1 (NM_001290397) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A-myb; G1-419-6; repro9
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228692 representing NM_001290397 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAAGAGGTCGCGCAGTGAGGACGAGGATGATGACCTTCAATATGCTGATCATGATTATGAAGTAC CTCAACAAAAAGGACTGAAAAAACTCTGGAACAGAGTAAAATGGACAAGAGATGAGGATGACAAGTTAAA GAAGTTGGTTGAACAACACGGAACTGATGATTGGACTCTAATTGCTAGTCATCTTCAAAATCGTTCTGAT TTTCAGTGCCAACATCGATGGCAGAAGGTTTTAAATCCAGAATTGATAAAGGGTCCTTGGACTAAGGAAG AAGATCAGAGGGTTATTGAATTAGTTCAGAAATATGGGCCAAAAAGGTGGTCTTTAATTGCAAAACATTT AAAAGGAAGAATAGGCAAGCAGTGCAGAGAAAGATGGCACAATCACCTGAACCCTGAAGTGAAGAAGTCT TCCTGGACAGAAGAAGAAGACAGGATCATATATGAAGCACACAAGCGCCTGGGAAACCGTTGGGCCGAGA TTGCTAAGTTACTTCCTGGAAGGACTGATAATTCTATCAAAAATCATTGGAATTCTACCATGCGAAGAAA AGTGGAACAGGAGGGCTATTTACAAGATGGAATAAAATCAGAGCGGTCTTCATCAAAACTTCAACACAAA CCTTGTGCGACTATGGACCATTTGCAAACCCAGAATCAGTTTTACATTCCTGTTCAGATCCCTGGGTATC AGTATGTGTCGCCTGATGGCAATTGTGTTGAACATGTTCAGACATCTGCCTTTATTCAGCAACCCTTTGT TGATGAAGATCCTGATAAAGAAAAAAAAATAAAGGAGCTCGAGTTGCTTCTTATGTCAGCCGAGAATGAA GTTAGAAGGAAGAGGCTTCCACCTCAACCTGGAAGCTTTTCTAGCTGGTCTGGTAGTTTCCTCATGGATG ATAGTATGTCTAACACACTGAATAATCTGGAGGAACACACTACTGAGTTTTATAGCATGGATGAAAATCA GACTGTTTCTGCTCAGCAGAATTCACCCACAAAGTTCCTAGCTGTAGAAGCAAATGCCGTGCTGTCTTCT CTACAGACCATCCCAGAATTCGCAGAAACTCTGGAACTAATTGAATCGGATCCTGTAGCATGGAGTGATG TTACCAGTTTTGATCTTTCTGATGCTGCTGCTTCTCCTGTCAAGTCTACTCCAGTTAAATTAATGAGAAT TCAACATAATGAAGGAGCCATGGAATGCCAGTTTAACGTCAGTCTTGTACTTGAAGGGAAAAAGAACAGT CGTAATGGTGGAGACAGTGAAGCTATTCCTTTAACATCCCCAAATGTGGTCAAGTTTAGCACTCCTCCAA CCATCCTCAGAAAGAAGAAAAGAATTCGAGTGGGTCAGTCTGCAGGCAGTGAGCTTGGCGATGGCTCACT TAGCGAAGTTGGTAATGCAGCACTCAAACACACACCAGTGAAAACACTACCATTTTCTCCTTCTCAGTTT TTTAACACATGTCCTGGAAATGAACAACTTAATATAGAAAACCCTTCCTTTACATCAACCCCAATTTGTG GGCAGAAAGTTCTCATTACAACTCCTCTTCAGAAGGAAGCAACCCCCAAAGATCAAAAAGAAAATGTAGG ATTCAGAACTCCTACTATTAGAAGATCTATACTGGGCACCACACCAAGAACTCCTACTCCTTTTAAGAAT GCACTTGCTGCTCAGGAAAAAAAATATGGACCTCTTAAAATTGTGTCCCAGCCACTTGCCTTTTTGGAAG AAGACATTCGAGAAGTTTTAAAAGAAGAAACTGGAACAGATATATTCCTCAAAGAGGAAGATGAACCTGC TTATAAAAGCTGCAAACAGGAGCACTCTGCATCTGTGAAGAAGGTCAGAAAATCCCTTGCCTTAGAGAGC TGGGACAAAGAAGAACCAGGGACTCAACTGCTAACTGAAGACATTTCAGACATGCAGTCAAATTGTGAAT GGGAAACAGTGGTATATGGGAAGACAGAAGACCAACTTATCATGACTGAACAAGCGAGAAGATATCTGAG TACTTACACAGCTACCAGCAGCACATCAAGAGCTCTAATACTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290397
Insert Size	2076 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290397.1, NP_001277326.1
RefSeq Size	4804 bp
RefSeq ORF	2076 bp
Locus ID	17864
Gene Summary	Transcription factor that specifically recognizes the sequence 5'-YAAC[GT]G-3' (PubMed:7813437, PubMed:23523368). Acts as a master regulator of male meiosis by promoting expression of piRNAs: activates expression of both piRNA precursor RNAs and expression of protein-coding genes involved in piRNA metabolism, such as PIWIL1 (PubMed:21750041, PubMed:23523368). The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons, which is essential for the germline integrity (PubMed:23523368).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.

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