Adgrg1 (NM_001198894) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Cyt28; Gpr56; TM7LN4; TM7XN1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228676 representing NM_001198894 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTCCAGGTGCTGCGGCAGATGGTCTACTTCCTACTGAGTCTGTTTTCTCTGGTGCAAGGTGCAC ACAGTGGCAGCCCCCGAGAAGACTTCCGCTTCTGTGGCCAGCGGAACCAGACCCAACAGAGCACCCTCCA CTATGATCAATCTTCAGAGCCTCACATCTTTGTGTGGAACACAGAGGAGACCCTCACAATTCGTGCCCCC TTCCTGGCAGCCCCAGATATTCCCCGCTTCTTCCCAGAGCCTAGAGGGCTCTATCACTTCTGCCTCTACT GGAGTCGCCACACTGGGAGACTCCACTTGCGCTATGGCAAGCATGACTACCTGCTTAGTAGCCAAGCCTC CAGACTCCTCTGCTTCCAGAAACAGGAGCAGAGCCTGAAGCAGGGAGCCCCGCTGATCGCCACCTCTGTC AGCTCCTGGCAGATTCCCCAGAACACCAGCCTGCCTGGGGCTCCGAGCTTCATCTTCTCCTTCCACAATG CCCCACACAAGGTCTCCCACAATGCATCTGTGGACATGTGTGATCTCAAGAAGGAATTGCAGCAGCTTAG CAGGTACCTGCAGCACCCTCAAAAGGCTGCCAAGCGGCCCACCGCAGCGTTCATCAGCCAGCAGTTACAG AGCCTGGAGTCAAAGCTGACCTCTGTGAGCTTCCTGGGAGACACATTATCCTTTGAGGAGGACCGGGTCA ATGCTACAGTGTGGAAGCTGCCACCCACAGCCGGTCTAGAGGATCTGCATATCCACTCCCAGAAGGAGGA GGAGCAGAGTGAGGTCCAGGCATACTCGCTGTTGCTTCCCCGGGCCGTATTCCAGCAGACCAGAGGCCGT CGCCGGGATGACGCCAAGAGGCTCCTGGTAGTAGACTTCAGCAGCCAAGCTTTGTTCCAGGACAAGAATT CTAGCCAAGTCCTGGGTGAGAAGGTCTTGGGTATTGTCGTGCAGAACACCAAAGTCACCAACCTCTCAGA TCCGGTGGTACTCACCTTCCAGCACCAGCCTCAGCCAAAAAATGTGACTCTGCAGTGCGTGTTCTGGGTT GAAGACCCGGCATCAAGCAGCACAGGGAGCTGGAGCAGTGCAGGCTGCGAGACAGTGAGCAGAGACACAC AGACATCCTGCCTGTGCAACCACCTGACCTACTTTGCAGTGCTGATGGTGTCATCCACAGAGGTAGAAGC CACTCACAAACACTACCTCACGCTCCTGTCCTACGTGGGCTGTGTCATCTCTGCTCTGGCTTGTGTCTTC ACTATTGCTGCCTACCTCTGCTCCAGGAGGAAGTCACGTGACTACACCATCAAAGTCCACATGAACCTGC TGTCCGCTGTCTTCCTGCTGGACGTGAGCTTCCTGCTCAGCGAGCCTGTGGCACTGACGGGCTCCGAAGC AGCCTGTCGCACCAGTGCCATGTTCCTGCACTTCTCCCTGCTTGCCTGCCTCTCCTGGATGGGCCTCGAG GGCTACAATCTCTACCGACTGGTGGTGGAGGTCTTCGGTACCTATGTGCCCGGCTATCTGCTCAAGCTGA GCATCGTGGGCTGGGGTTTTCCTGTCTTCCTGGTCACTCTGGTGGCGTTGGTGGATGTGAATAACTACGG CCCCATTATCCTAGCTGTGCGCCGGACTCCGGAACGTGTCACCTACCCCTCTATGTGCTGGATCCGGGAC TCCCTGGTGAGCTATGTCACCAACCTGGGCCTCTTCAGTCTGGTGTTCCTGTTCAACCTGGCTATGCTGG CCACCATGGTGGTGCAGATCCTGCGGCTTCGCCCGCACAGCCAGAACTGGCCCCACGTGCTGACCCTGCT GGGCCTCAGCCTGGTCCTTGGCCTCCCCTGGGCCTTGGTCTTCTTTTCCTTTGCTTCCGGCACCTTCCAG CTTGTCATCCTCTACCTCTTCAGCATCATAACTTCCTTCCAAGGCTTCCTCATCTTCCTGTGGTACTGGT CCATGCGGTTCCAGGCCCAAGGCGGCCCCTCCCCTCTGAAGAACAACTCAGACAGCGCCAAACTCCCCAT CAGCTCCGGCAGCACCTCCTCCAGCCGCATCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001198894
Insert Size	2064 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001198894.1, NP_001185823.1
RefSeq Size	3605 bp
RefSeq ORF	2064 bp
Locus ID	14766
UniProt ID	Q8K209
Gene Summary	Receptor involved in cell adhesion and probably in cell-cell interactions. Mediates cell matrix adhesion in developing neurons and hematopoietic stem cells. Receptor for collagen III/COL3A1 in the developing brain and involved in regulation of cortical development, specifically in maintenance of the pial basement membrane integrity and in cortical lamination (PubMed:21768377). Binding to the COL3A1 ligand inhibits neuronal migration and activates the RhoA pathway by coupling to GNA13 and possibly GNA12 (By similarity). Plays a role in the maintenance of hematopoietic stem cells and/or leukemia stem cells in bone marrow niche (PubMed:23478665). Plays a critical role in tumourigenesis (By similarity). Plays essential role in testis development (PubMed:20981830).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.

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