Dpysl3 (NM_001291455) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CRMP; CRMP-4; DRP-3; TUC4; U; Ul; Ulip; ULIP-1; Ulip1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228666 representing NM_001291455 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCGGGCCGAAGGGGTTGGGACAGCTCCCACGAGGACGACCTGCCTGTGTACTTGGCCCGTCCGG GCACCACAGATCAGGTCCCACGGCAGAAGTACGGTGGCATGTTCTGCAACGTGGAGGGTGCCTTCGAGAG CAAGACGTTGGATTTTGATGCCCTGAGTGTGGGACAGCGCGGCGCCAAGACTCCCCGGAGCAGCCAGGGC AGCGGCCGTGGCGCAGGGAACCGGCCCGGGATGGAAGTGGACGCGCGCAGGGGCCCGGGCCGGGAGGAGA GCAGGGAACCCGCGCCGGAGTCCCCCAAGCCCGCTGGGGTGGAGATCCGGAGCGCCACCGGCAAGGAGGT CTTGCAGAACCTTGGTCCCAAGGACAAGAGTGACCGTCTTCTAATCAAGGGAGGGAGAATCGTCAATGAT GATCAGTCCTTTTATGCTGATATTTACATGGAGGATGGCTTAATAAAGCAAATTGGAGACAATCTGATTG TCCCCGGAGGTGTGAAGACCATCGAGGCCAACGGGAAGATGGTGATCCCTGGAGGCATTGACGTCCATAC CCACTTCCAGATGCCTTACAAGGGAATGACCACAGTGGACGATTTCTTCCAAGGGACAAAGGCTGCCTTA GCGGGAGGCACCACCATGATCATTGACCACGTGGTACCTGAACCTGAGTCTAGCCTGACAGAGGCCTATG AAAAGTGGCGTGAGTGGGCCGATGGGAAGAGCTGCTGTGACTATGCTTTGCATGTGGACATCACCCACTG GAATGACAGCGTCAAGCAGGAGGTACAGAGCCTCAGCAAGGAAAAAGGCGTTAACTCCTTCATGGTTTAC ATGGCCTACAAGGATTTATATCAAGTGTCCAACACAGAGCTCTATGAGATCTTCACCTGCCTGGGAGAGC TGGGGGCCATTGCTCAAGTTCATGCCGAGAATGGAGATATCATTGCCCAGGAGCAGGCACGGATGCTGGA AATGGGGATAACCGGCCCAGAAGGCCACGTTCTGAGCAGACCGGAAGAGCTGGAAGCTGAGGCTGTGTTC CGTGCCATCACCGTCGCCAGCCAGACCAACTGCCCCCTCTATGTCACCAAGGTCATGAGCAAGAGCGCGG CTGATCTCATCTCACAAGCCAGGAAGAAAGGAAATGTGGTCTTTGGCGAGCCCATCACTGCCAGCCTGGG AATAGATGGAACTCATTACTGGAGTAAGAACTGGGCCAAAGCAGCTGCGTTTGTGACATCCCCACCTCTG AGCCCTGACCCTACCACACCTGACTACATCAACTCCTTGCTGGCCAGCGGGGATCTGCAGCTCTCTGGAA GTGCCCACTGTACCTTTAGCACTGCCCAGAAAGCCATTGGGAAGGACAACTTCACAGCCATCCCTGAAGG CACCAATGGCGTGGAGGAGCGTATGTCTGTCATCTGGGACAAGGCTGTGGCCACGGGGAAGATGGATGAA AACCAGTTTGTGGCCGTGACAAGCACCAATGCTGCCAAGATATTCAACCTGTACCCTCGAAAGGGGAGAA TAGCTGTGGGCTCTGACAGCGACCTTGTCATCTGGGATCCAGATGCCTTGAAGATTGTCTCTGCCAAGAA CCACCAGTCGGTTGCCGAATACAACATCTTTGAAGGGATGGAGCTGCGTGGTGCACCTCTGGTGGTTATC TGCCAGGGCAAGATCATGCTGGAAGATGGCAACCTGCACGTGACCCAGGGGGCTGGCCGCTTCATTCCCT GCAGCCCATTCTCTGACTATGTCTATAAGCGCATTAAAGCAAGGAGGAAGATGGCAGACCTGCATGCAGT CCCAAGAGGCATGTATGATGGACCAGTGTTTGACTTGACCACCACCCCCAAGGGGGGCACCCCAGCTGGC TCTACTCGGGGCTCTCCCACTCGGCCAAACCCGCCAGTGAGGAACCTCCATCAGTCAGGATTTAGCCTGT CAGGCACCCAAGTGGATGAGGGTGTCCGCTCAGCTAGCAAACGCATTGTGGCACCCCCTGGAGGCCGTTC TAACATCACATCCCTGAGTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291455
Insert Size	2052 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291455.1, NP_001278384.1
RefSeq Size	5502 bp
RefSeq ORF	2052 bp
Locus ID	22240
Gene Summary	This gene encodes a protein that belongs to the TUC (TOAD-64/Ulip/CRMP) family of proteins. Members of this family are phosphoproteins that function in axonal guidance and neuronal differentiation during development and regeneration of the nervous system. A mutation in the human gene is associated with amyotrophic lateral sclerosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2014] Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (CRMP4b, also known as isoform 3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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