Rph3a (NM_001302345) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2900002P20Rik; AU022689; AW108370
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228659 representing NM_001302345 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGACACTGTGGTGAACCGATGGATGTACCCTGGTGATGGCCCTCTGCAGTCAAATGACAAGGAAC AGCTGCAGGCAGGATGGTCCGTCCATCCTGGAGCACAGACCGACAGGCAGAGGAAGCAGGAAGAACTGAC AGACGAGGAGAAGGAGATCATCAACAGAGTGATTGCTCGGGCAGAGAAGATGGAAGCCATGGAACAGGAA CGCATTGGGCGCCTGGTGGACCGTCTGGAGACCATGAGGAAGAATGTGGCTGGAGATGGCGTGAACCGCT GCATTCTGTGTGGGGAACAGCTGGGTATGCTGGGCTCGGCCTGTGTCGTGTGTGAAGACTGTAAGAAGAA TGTCTGCACCAAGTGTGGGGTTGAGACCTCCAACAACCGTCCGCATCCGGTATGGCTCTGCAAGATCTGC CTTGAGCAGAGAGAGGTCTGGAAGCGCTCAGGAGCATGGTTCTTCAAAGGTTTCCCCAAGCAGGTCCTTC CACAGCCCATGCCTATAAAGAAGACCAAGCCCCAGCAGCCTGCTGGTGAACCGGCCACCCAGGAGCAGCC TACACCTGAGTCCAGGCATCCAGCCAGGGCTCCAGCTCGAGGTGACATGGAGGACAGGAGGCCCCCAGGG CAGAAGCCAGGCCCTGACCTCACCTCTGCTCCTGGGAGAGGAAGCCATGGGCCTCCCACGCGTAGGGCCT CTGAGGCACGGATGAGTACAGCCGCCCGGGATTCTGAGGGCTGGGACCATGCCCATGGTGGGGGTACTGG AGACACCAGCCGTAGCCCAGCAGGTTTGAGGCGAGCTAACTCAGTCCAGGCAGCCCGCCCTGCCCCAGCC CCAGTGCCAAGCCCAGCACCTCCTCAGCCGGTGCAGCCAGGGCCCCCTGGGGGCAGCAGGGCCACTCCTG GGCCAGGACGCTTTCCGGAGCAGAGCACAGAGGCTCCTCCAAGTGACCCTGGCTATCCAGGGGCTGTCGC CCCAGCCCGAGAGGAGAGGACAGGACCTGCGGGGGGCTTCCAGGCAGCGCCGCACACTGCAGCCCCCTAT TCCCAGGCAGCCCCTGCTCGCCAGCCACCACCTGCGGAGGAGGAGGAGGAAGAAGCCAATAGTTATGACT CTGATGAAGCAACCACACTGGGTGCCCTGGAATTCAGCCTTCTCTATGACCAAGACAACAGCAACCTGCA GTGCACCATCATCAGGGCGAAGGGACTGAAGCCCATGGATTCCAATGGCTTGGCAGATCCCTATGTGAAG CTTCATCTGCTGCCTGGAGCCAGCAAGTCCAACAAGCTTCGTACAAAGACCCTGCGCAACACTCGGAACC CTGTGTGGAATGAGACACTGCAGTATCATGGCATTACAGAGGAGGACATGCAGAGGAAGACACTAAGGAT CTCCGTGTGTGACGAGGACAAGTTTGGCCACAACGAGTTCATTGGTGAGACCAGGTTCTCGCTCAAGAAG CTGAAGGCTAACCAGAGGAAAAACTTCAACATCTGCCTGGAGCGGGTGATCCCGATGAAGAGAGCAGGGA CCACCGGGTCGGCCCGTGGCATGGCTCTCTATGAGGAGGAGCAGGTAGAGCGGATCGGCGATATAGAGGA ACGGGGCAAGATCCTGGTGTCCCTCATGTACAGCACGCAGCAGGGCGGCCTCATTGTGGGAATCATCCGC TGTGTGCACCTGGCCGCCATGGATGCCAACGGCTACTCAGACCCCTTTGTCAAGCTCTGGCTGAAACCGG ACATGGGGAAGAAAGCCAAGCACAAGACTCAGATTAAAAAGAAGACCCTGAATCCCGAGTTTAACGAGGA GTTCTTTTATGATATCAAACACAGCGACCTGGCTAAAAAGTCCCTGGATATCTCGGTGTGGGACTACGAC ATTGGCAAGTCTAATGATTACATCGGAGGCTGCCAGCTGGGGATCTCGGCCAAAGGCGAGCGCTTGAAAC ATTGGTATGAGTGTTTGAAGAACAAAGACAAGAAGATTGAGCGCTGGCACCAACTGCAGAACGAGAACCA CGTGTCCAGTGATTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001302345
Insert Size	2046 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302345.1, NP_001289274.1
RefSeq Size	3918 bp
RefSeq ORF	2046 bp
Locus ID	19894
UniProt ID	P47708
Gene Summary	Plays an essential role in docking and fusion steps of regulated exocytosis (By similarity). At the presynaptic level, RPH3A is recruited by RAB3A to the synaptic vesicle membrane in a GTP-dependent manner where it modulates synaptic vesicle trafficking and calcium-triggered neurotransmitter release (By similarity). In the post-synaptic compartment, forms a ternary complex with GRIN2A and DLG4 and regulates NMDA receptor stability. Plays also a role in the exocytosis of arginine vasopressin hormone (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.

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