Esrp1 (NM_001290383) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2210008M09Rik; A630065D16; BC031468; Rbm35a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228654 representing NM_001290383 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGCGTCTCCGGATTACTTGGTGGTGCTTTTTGGGATCACTGCTGGGGCCACCGGAGCCAAGCTGG GTTCGGATGAGAAGGAGCTGATCCTGCTGTTATGGAAAGTCGTGGATTTGGCCAACAAGAAGGTGGGACA GCTGCACGAAGTACTAGTTAGACCGGATCAGTTGGAACTGACAGAGGATTGTAAAGAAGAAACTAAAATA GACGCCGAAAACCTGTCTTCCGCGCCGCAGCTGGACCAAGCCCTCCGACAGTTTAACCAGTCAGTGAGCA ATGAACTGAACATCGGGGTAGGGACTTCCTTCTGTCTCTGTACTGACGGGCAGCTTCACATTAGGCAGAT CTTGCATCCCGAGGCTTCCAAGAAGAATGTGTTACTGCCTGAGTGCTTCTATTCCTTTTTCGATCTTCGG AAAGAGTTCAAGAAGTGCTGCCCGGGCTCTCCCGATATCGACAAGCTGGATGTCGCAGCGATGACAGAAA GCCTGAACTTTGAGAAGAGTGACTCTGTGTCCCGATACGGAGCCTCCCAAGTTGAAGACATGGGGAACAT AATTTTAGCAATGATCTCCGAGCCCTACAATCACAGATTTTCAGATCCAGAGAGAGTAAACTACAAGTTT GAAAGTGGCACTTGCAAGATGGAACTTATTGATGACAGCACAGTGGTGAGAGCTCGAGGTTTGCCCTGGC AATCTTCAGATCAAGATATTGCAAGGTTCTTCAAAGGACTCAATATTGCCAAGGGTGGCGCAGCACTTTG CCTGAATGCTCAGGGCCGGAGGAACGGAGAAGCTCTGGTTAGATTTGTAAGTGAGGAGCACAGAGATCTA GCCCTGCAGAGGCACAAACATCACATGGGGACCCGGTACATTGAGGTTTACAAAGCAACAGGCGAAGATT TCCTTAAAATTGCCGGAGGGACATCCAATGAGGTAGCCCAGTTTCTCTCCAAGGAAAACCAAGTCATCGT GCGCATGAGGGGGCTCCCTTTCACGGCCACGGCTGAAGAAGTGGTGGCCTTCTTTGGACAGCACTGCCCC ATCACTGGAGGGAAGGAAGGCATCCTCTTCGTTACCTACCCAGATGGTAGGCCAACCGGAGATGCTTTTG TGCTCTTTGCTTGTGAGGAGTATGCTCAGAACGCCCTGAGGAAGCACAAGGAGTTGCTGGGTAAAAGATA CATCGAGCTCTTCAGGAGCACAGCTGCCGAAGTTCAGCAGGTGCTGAACCGATTCTCCTCAGCCCCTCTG ATTCCACTTCCAACCCCTCCCATTATCCCTGTACTACCTCAGCAGTTTGTGCCTCCCACGAATGTCAGAG ACTGCATACGTCTGCGAGGTCTTCCCTATGCTGCCACAATAGAAGACATTCTGGACTTCCTGGGGGAGTT TTCCACAGATATTCGTACACATGGAGTGCACATGGTATTGAATCACCAGGGACGCCCGTCAGGAGATGCC TTTATCCAGATGAAGTCAACAGACAGAGCATTCATGGCTGCGCAGAAGTACCATAAAAAAACCATGAAGG ACAGATATGTTGAAGTCTTTCAGTGTTCAGCTGAGGAGATGAACTTTGTGTTAATGGGGGGCACTTTAAA TCGAAATGGCTTGTCCCCACCGCCATGTAAGTTACCATGCCTGTCTCCTCCCTCCTACACATTCCCAGCT CCCACGGCTGTGATTCCTACCGAAGCTGCCATTTATCAACCTTCTCTGCTCTTGAACCCACGGGCACTGC AGCCCTCCACTGCATACTACCCGGCAGGCACTCAGCTCTTCATGAACTACACAGCCTACTATCCCAGCCC CCCAGGTTCGCCTAATAGTCTTGGCTACTTCCCTACAGCTGCTAATCTTAGCAGTGTCCCGCCCCAGCCT GGCACCGTGGTCAGAATGCAGGGCCTGGCCTACAATACTGGAGTTAAGGAAATTCTTAACTTCTTCCAAG GTTACCAGTATGCAACCGAGGATGGACTTGTACACACAAATGACCAGGCCAGGACTCTACCCAAAGAATG GGTTTGTATTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290383
Insert Size	2043 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290383.1, NP_001277312.1
RefSeq Size	3966 bp
RefSeq ORF	2043 bp
Locus ID	207920
UniProt ID	Q3US41
Gene Summary	mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2 (By similarity). Regulates splicing and expression of genes involved in inner ear development, auditory hair cell differentiation, and cell fate specification in the cochlear epithelium (PubMed:29107558).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter by an amino acid, compared to isoform 1.

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