Zbtb33 (NM_001079513) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW260253; E130014G12Rik; Kaiso; Znf-kaiso
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228633 representing NM_001079513 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGTAGAAAACTGATTTCTGCTACAGACATTCAGTACTCTGCCAGTCTGCTGAACTCCTTGAATG AACAGCGTGGCCATGGTCTCTTTTGTGATGTTACTGTTATTGTGGAAGACCGAAAATTCCGGGCCCATAG GAACATCCTTTCAGCCTCGAGTACATACTTCCATCAGCTCTTCTCAGTTGCTGGGCAAGTTGTTGAACTA AGCTTTATAAGAGCTGAGATTTTTGCAGAAATTCTGAACTATATCTACAGTTCCAAAGTTGTCCGTGTTA GAGCAGATTTGCTTGACGAGTTAATTAAATCAGGGCAGTTACTAGGCGTGAAATTTATAGCAGAGCTTGG TGTCCCACTGTCACAGGTTAAAAGCATATCAGGTACAGAACAGGATGGTACTGCTGAGACCTTACCTTCT AGTTCTAGTGACAAAAGCCTTGACATGGAAAAATCAAAAGATGAAGCCCAAGATAATGGGGCTACAGTAA TGCCCATTATAACTGAGTCTTTTTCACTATCTGCTGAAGATAATGAAATGAAAAAGATCATTGTTACTGA CTCAGATGATGATGATGATGACGATGTCATTTTCTGCTCTGAAATTTTGCCTGCAAAGGAGGATTTGCCA AGTAACAACACAGCAACACAGGTCCAGCCTAACCCAGCCTCTGTTGCTATTTCGGAAGTGACACCTTGCG CTAGCAATAACTCGCCCCCTGTAACAAACATCACACCTACTCAACTTCCCACTCCTGTGAATCAGGCAAC TCTAAGCCAAACACAAGGAAGTGAAGAATTGCTAGTGTCTTCAGCTTCAACACATCTGACTCCTAACATT ATTTTGTTAAATCAGGCTCCACTTACTGCACCACCGAGTGCCAGTTCTTCACTCCCAAATCATATGTCCT CTTCAGTCAATGTCCTTGTACAGAATCAGCAGACGCCTAACAGTGCTGTTTTAACAGGAAACAAGGCTGA GGAAGAGGAAGAAATTATAGATGATGATGATGACATCATTAGCTCCAGTCCAGACTCAGCAGTCAGTAAT ACATCTCTGGTCCCTCAGGCTGATAACTCCAAAAGTACCACTTTAGATGGATCATTGACACAGAAGATGC AGATTCCTGTACTTCCTCAAGAGCCACCTTCTAATTCTTTAAAAATTTCAGATGTCATTACTAGAAACAC TAATGATCCAGGCTTAAGGTCAAAACATGTAATGGAGGGTCAGAAGATCATTACATTAGATACAGCTACT GAAATCGAAGGCTTGTCAACTGGTTGCAAGGTTTATGCAAATATCGGTGAAGATACTTACGATATAGTGA TCCCTGTCAAAGATGACCCTGATGGAGGGGAGGCCAAACTTGATAATGAGCTACCAAAAACATCTGGCAG TGAGCCACCAAACAAGCGTATGAAAGTAAAACATGATGATCACTATGAGTTAATAGTAGATGGAAGGGTC TATTATATTTGCATTGTATGCAAAAGGTCCTATGTCTGTCTGACAAGCTTGCGAAGACATTTTAACATTC ATTCTTGGGAGAAGAAGTATCAGTGCCGTTACTGTGATAAAGTATTTCCTCTTGCAGAATACCGCACCAA GCATGAAATCCATCACACAGGGGAGCGAAGGTATCAGTGTTTAACGTGTGGCAAATCTTTCATCAACTAT CAGTTTATGTCTTCACACATAAAGTCAGTTCATAGTCAAGATCCTTCTGGGGACTCAAAGCTTTATCGCT TACATCCATGCAAGTCTTTACAGATCAGACAGTATGCATACCTTTCTAATAAGTCAAGTGCTATGCCTGT CATGAAGGATGATGCTGTTGGATACAAGGTTGATGCTGGAAAAGAGCCTCCAGTAGGGACCACATCTACT CCTCCTCAGAACAAGTCAACGTTCTGGGAAGATATTTTTATTCAGCAAGAAAATGATTCCATTTTTAAAC AGAATGTAACCGATGGCAGTACTGAGTTTGAATTTATAATACCAGAATCTTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001079513
Insert Size	2016 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001079513.1, NP_001072981.1
RefSeq Size	4948 bp
RefSeq ORF	2016 bp
Locus ID	56805
UniProt ID	Q8BN78
Gene Summary	Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). May recruit the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. Contributes to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1 (By similarity).[UniProtKB/Swiss-Prot Function]

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