Kif2c (NM_001290662) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4930402F02Rik; ESTM5; Knsl6; MCAK; X83316
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228631 representing NM_001290662 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTGACATTGACGATGTGGCTGCAATAAACCCAGAGCTCCTACAGCTTCTTCCCTTACGACCGAAGG ACAGTCTGCCACTGCAGGAAAACGTAACGGTCCCGAAGCAAAAACGCAAATCAGTCAACTCCAAAATTCC AGCTCTAAAAGAAGGCCTTCGAAGCCGCTCCACTCGCATGTCCACTGTCTCAGAAGTTCGAATCCCTGCT CAGGAGAATGAAATGGAGGTGGAGCTGCCTGTGCCTACAAACTCTCGCAAGCAGTTTGCCATTCCCAGCC ACCCTCGGGCCTCCTGCTCCACAGTGACAGAATTGCCGTTGTTGATGGTCAGTGAGGAGGCAGAAGAGCA AGCCCACTCCACCCGCAGTACCTCATCTGCAAACCCTGGGAACTCAGTTCGGAGGAAATCGTGTATCGTG AAGGAGATGGAGAAAATGAAGAACAAGCGTGAAGAGAAGAGGGCCCAGAACTCGGAGCTAAGAATAAAGC GAGCACAGGAATATGACAGCAGCTTTCCAAACTGGGAATTTGCCCGGATGATCAAAGAATTTCGGGTTAC TATGGAATGTAGCCCGCTTACTGTGACAGACCCTATCGAAGAGCACAGGATCTGTGTCTGTGTCAGGAAA CGCCCACTGAATAAACAAGAACTGGCCAAGAAAGAAATCGATGTGATTTCTGTTCCCAGCAAGTGTCTCC TCCTGGTGCATGAACCCAAGTTAAAAGTGGACTTAACGAAGTACCTGGAGAACCAAGCTTTCTGCTTTGA CTTTGCATTTGATGAGACAGCTTCCAATGAAGTTGTCTACAGGTTCACAGCAAGGCCACTGGTACAGACG ATTTTTGAAGGGGGAAAAGCAACATGCTTTGCCTATGGACAGACAGGCAGTGGGAAGACACATACAATGG GTGGAGATCTGTCTGGTAAAAGTCAGAACGCATCTAAAGGGATCTACGCCATGGCTTCCCGGGATGTCTT CCTTCTGAAGAACCAGCCTCGGTACCGGAACTTAAACCTGGAAGTTTATGTGACATTCTTCGAGATCTAC AATGGGAAGGTGTTTGATCTGCTCAACAAAAAGGCCAAGTTACGTGTGCTGGAGGACAGCAGGCAGCAGG TGCAGGTTGTGGGACTGCAGGAGTACCTGGTCACCTGTGCTGATGATGTCATCAAGATGATCAACATGGG CAGTGCCTGCAGGACTTCTGGACAAACATTTGCCAACTCCAATTCCTCCCGCTCCCACGCCTGCTTCCAG ATTCTTCTTCGAACTAAAGGGAGATTACATGGCAAATTCTCTTTGGTGGATCTGGCAGGGAATGAACGAG GGGCCGACACATCTAGTGCTGACCGGCAGACTCGCATGGAGGGAGCAGAAATTAACAAGTCTCTCCTGGC TCTGAAGGAGTGCATCCGGGCGCTGGGACAGAACAAGGCTCACACCCCGTTCCGTGAGAGCAAGCTGACC CAGGTGCTGAGGGACTCGTTCATTGGGGAGAACTCGAGGACCTGCATGATCGCCATGATCTCACCAGGCA TAAGCTCCTGTGAATACACCTTAAACACGCTGAGATACGCAGACAGAGTCAAGGAGCTAAGCCCCCACAG CGGACCCAGTGGAGAGCAGCCGGTTCAGATGGAAACAGAAGTGATGGAAGCCAGCTCTAATGGGACGTCT CTGACTGGCAATGAAGAGGAGGAGCTGTCTTCCCAGATGTCCAGCTTTAATGAAGCCATGACTCAGATCA GGGAGCTGGAAGAGAGGGCCCTGGAAGAGCTCAGAGAGATCATACAGCAAGGACCAAACTGGCTTGAGCT CTCCGAGATGACAGATCAGCCAGACTATGACTTGGAGACCTTTGTGAACAAAGCAGAATCTGCCCTGACA CAGCAGGCTAAGCAGGCGAAGCACTTCTCAGCCCTTCGAGAAGTCATCAAGGCCCTGCGCCTAGCCATGC AACTGGAAGAACAGGCTAGCAAACAAATAAACAGCAAGAAACGGCACCAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290662
Insert Size	2013 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290662.1, NP_001277591.1
RefSeq Size	2792 bp
RefSeq ORF	2013 bp
Locus ID	73804
Gene Summary	In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells (PubMed:14980225). Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 2, which has a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.