Cnga3 (NM_001282010) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CNG3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228623 representing NM_001282010 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAAGGTGAACACCCAGTGTTCACAGCCCTCCCCGACCCAACTTTCAATAAAGAATGCGGACAGAG ATCTCGACCACGTAGAGAACGGCCTGGGCAGGTCTCACTCACCATGTGAGGAGACATCTTCCACACTGCA GCAAGGGATCGCCATGGAGACCCGAGGACTGGCTGGATCCGCACGGAGCGTAGTCACAAGCCAGGGACCA GCCAGGGTGTCACGCCTCATCATCTCGATTCGTGCGTGGGCCTCCAGGCACTTACACGATGAAGACCAGA CACCTGACTCCTTTTTGGATCGATTTCATGGATCTGAGCTTAAGGAAGTCTCCACCCGGGAAAGCAATGC CCAGCCCAACCCAGGAGAACAGAAGCCACCAGACGGAGGGGAAGGCAGGAAGGAGGAGCCCATTGTGGTG GACCCCTCCAGCAACATCTACTACCGCTGGCTGACTGCCATCGCGCTCCCAGTCTTCTATAACTGGTGTC TACTTGTATGCAGGGCCTGTTTTGATGAGCTACAATCAGAGCACCTGACGCTGTGGCTGGTCTTGGACTA CTCTGCAGATGTCCTTTATGTTTTAGACATGCTGGTTCGAGCCCGGACAGGTTTCCTTGAGCAAGGCCTA ATGGTCAGAGATACCAAGAGGCTGTGGAAACATTACACAAAGACCTTGCACTTCAAGCTGGACATCCTGT CTCTCATCCCCACAGACCTGGCTTATTTAAAGTTGGGCGTGAACTACCCAGAACTGAGGTTCAACCGCCT CCTGAAGTTCTCTCGGCTCTTTGAATTCTTTGACCGCACAGAGACAAGGACCAACTACCCCAACGTGTTC AGGATAGGGAACTTGGTCCTTTACACACTCATCATCATCCACTGGAATGCCTGCATCTACTTTGCCATTT CCAAGTTCATTGGTTTTGGGACAGACTCCTGGGTCTATCCAAACACCTCCAAGCCGGAGTATGCACGACT CTCCCGGAAGTACATTTACAGTCTCTACTGGTCCACCCTGACCCTGACCACCATTGGTGAGACCCCACCC CCCGTGAAAGATGAAGAGTATCTCTTTGTGGTCATCGACTTCCTGGTGGGTATCCTGATCTTTGCCACCA TAGTGGGCAACGTAGGCTCCATGATTTCCAACATGAACGCTCCCCGGGTAGAGTTCCAGGCTAAGATCGA CTCCGTCAAGCAGTACATGCAGTTCCGGAAGGTAACCAAGGACTTGGAGACTCGGGTTATCCGGTGGTTT GACTATCTGTGGGCCAACAGGAAGACGGTGGATGAAAAGGAAGTGCTCAAAAACCTCCCGGACAAGCTGA AGGCTGAGATCGCCATCAACGTGCACCTGGACACGCTGAAGAAGGTCCGAATCTTCCAGGACTGCGAGGC GGGGCTGCTGGTGGAGCTGGTGCTGAAGCTCCGCCCCACTGTGTTCAGCCCCGGGGACTACATATGCAAA AAGGGGGACATTGGAAGAGAGATGTACATCATCAAGGAGGGCAAGCTGGCTGTGGTGGCTGATGACGGGG TCACCCAGTTTGTGGTCCTCAGTGACGGCAGTTACTTTGGGGAGATTAGCATCTTAAACATTAAGGGGAG CAAGTCTGGGAACCGCAGGACGGCCAACATCAGGAGTATCGGCTACTCAGACCTGTTCTGCCTCTCCAAG GATGACCTAATGGAAGCCCTCACCGAGTACCCAGACGCTAAGAGGGCTCTGGAGGAAAAGGGCCGTCAGA TTCTGATGAAGGACAACCTAATTGATGAGGACCTAGTCGCGGCCAGGGTAGATACCAGGGACGTTGAGGA GAAGGTGGAGTACCTGGAGTCGTCCCTGGACATCCTGCAGACGAGGTTTGCCCGACTCCTGGCTGAGTAC AGTGCCTCCCAGATGAAGCTGAAACAGCGGCTCACTCGGCTGGAGAGCCAGATGAACAGGAGGTGTTGTG GCTTCTCACCTGACAGGGAGAATTCCGAGGATGCTTCAAAGACTGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282010
Insert Size	2010 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282010.1, NP_001268939.1
RefSeq Size	3623 bp
RefSeq ORF	2010 bp
Locus ID	12790
UniProt ID	Q9JJZ8
Gene Summary	Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cyclic GMP which leads to an opening of the cation channel and thereby causing a depolarization of cone photoreceptors. Essential for the generation of light-evoked electrical responses in the red-, green- and blue sensitive cones (By similarity). Induced a flickering channel gating, weakened the outward rectification in the presence of extracellular calcium, increased sensitivity for L-cis diltiazem and enhanced the cAMP efficacy of the channel when coexpressed with CNGB3.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1).

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