Pus7 (NM_001289780) Mouse Untagged Clone

CAT#: MC228612

Pus7 (untagged) - Mouse pseudouridylate synthase 7 homolog (S. cerevisiae) (Pus7), transcript variant 1



  "NM_001289780" in other vectors (1)

CNY 6,460.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms C330017I15Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228612 representing NM_001289780
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAATGACAAGCACGTCCCTGAAACGTGGGTGCCTTGTTGTGGAAGATAATGACAGTGTGACCCCAC
ACGACGAAACAAAGAAACAGAAGGTGTCCGAGGGCTGTCTGACCAGCAGTCAGGATGGGGTAGAGAATGA
CGGTCTACACCGCAGTGAGAACGAGCCTGGGCCTCCAGAAGCTGAAAGCACCGTGAAAGATGACGAGAAT
TCCAGCGCTCAGGTGCAAGAGGAAGAGGAGGAGGAGGAGGAAGAAGACGGCCTTTCAGAAGCAGGGGAAG
AGGAAGAAGCAGAGAGTTTTGCAGACATGATGAAACATGGGCTCACAGAACTTGATGTGGGCATCTGCAA
GTTCGTGAGTTCCCATCACGGCTTCTCGGGGATCTTAAAAGAAAGATACTCTGACTTTGTCGTTCACGAA
ATCGGGAAAGATGGACGGATCAGCCATTTGGATGACTTATCTGTTCCGGTGGATGAAGAGGACCCTCCAG
AAGACGCTCTCACTGTCTTGACAGCTGAGGACAGGCAGCAGTTGGAGGAGCTCCAGCTTTTCAAAAATAA
AGAAACCAGTGTTGCCATCGAGGTCATTGAGGACACCAAAGAGAAGCGAACAGTCATCCATCAAGCCATT
AAATCTCTCTTCCCAGGACTAGAGACAAAGACAGAGGATAGAGAGGGGCGGAAATACATCGTAGCCTACC
ATGCAGCTGGCAAAAAGGCTTTGGCAAAGGTCAGAGCTGCTGCAGATCCAAGAAAACATTCTTGGCCAAA
ATCCCGGGGAAGTTACTGCCATTTTGTTCTCTACAAAGAAAACAAAGACACCATGGATGCCATCAATGTT
CTATCCAAGTACCTGCGGGTAAAGCCAAACATATTCTCCTATATGGGAACCAAAGACAAAAGGGCAATCA
CAGTCCAGGAGATTGCTGTTCTCAAAATAAGTGCACAAAGACTTGCCCACCTCAATAAGTGCCTGATGAA
CTTGAAGCTCGGGAATTTCAGCTATCAGAAAACCCCTCTGAAGTTAGGAGCGCTCCAAGGAAACCACTTC
ACTGTTGTTCTCAGGAATATAACAGGAACTGATGAGCAGGTGCAGCAAGCCATGCAGTCTCTGAGGGAGA
CCGGCTTCATTAACTACTATGGCATGCAAAGGTTCGGGACCACAGCTGTTCCAACGTACCAGGTTGGAAG
AGCAATTCTACAGAATTCCTGGACAGAAGTCATGGATTTAATACTGAAACCCCGTTCTGGAGCTGAGAAG
GGTTACTTGGTGAAATGCAGAGAAGAATGGGCCAAGACCAAAGACCCAGCCTCTGCTCTCAAAAAGCTGC
CCGTCAAAAGGTGTGTGGAAGGGCAGCTGCTCCGAGGACTTTCCAGATATGGAATGAAGAATATAGTCTC
TGCATTTGGCATAATTCCAAGAAACAATCGCTTAATGTACATCCACAGTTATCAGAGCTACGTGTGGAAC
ACCATGGTGAGCAGAAGAATTGAGGAGTACGGGCTGAGGCCTGTGCCCGGGGACCTTGTTCTCAAAGGAG
CCACGCCCACCTACATTGAGGAAGATGACGTTGACAACTACTCCATCCACGATGTGGTGATGCCTTTGCC
TGGGTTTGATGTTATCTACCCCAAGCATAAAATCAGCGAGGCCTACAGGGAAATGCTTGCTGCAGACAAC
CTTGATATTGACAACATGAGACACACGATTCGAGATTACTCCTTATCGGGGGCCTACCGAAGGATCATCA
TCAGGCCTCAGAGCGTCAGCTGGGAAGTTGTTGCATATGATGATCCTAAAATTCCACTTTTCAACACGGA
TGTGGACAACCTCGAGGGGAAACCACCACCAGTGTTTGCTTCTGAAGGCAAATACAGGGCTCTGAAGATG
GACTTTTCTCTTCCTCCTTCTACTTACGCCACCATGGCCATTCGAGAAGTGCTCAAAATGGACACCAGCA
TCAAGAACCAGACCCAGCTAAATACGAGCTGGCTGCGATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001289780
Insert Size 2001 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001289780.1, NP_001276709.1
RefSeq Size 3925 bp
RefSeq ORF 2001 bp
Locus ID 78697
UniProt ID Q91VU7
Gene Summary Pseudouridylate synthase that catalyzes pseudouridylation of RNAs. Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex. Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3'. In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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