Ralbp1 (NM_001198949) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Rip1; RLIP76
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228575 representing NM_001198949 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGAGTGCTTCCTGCCCCCGAGCAGCAGCCCTAGTGAACACCGCAGGGCCGAGCATGGCAGCGGGC TGACCCGGACCCCCAGCTCTGAGGAGATCAGTCCTACAAAGTTCCCTGGATTGTACCGGACAGGCGAGCC CTCGCCTCCCCATGACGTCCTCCATGAGCCTCCTGATACAGTGTCAGATGACGACAAAGACCATGGAAAG AAAAAAGGGAAATTTAAGAAAAAGGAGAAAAGGACTGAAGGCTACGCTGCCTTCCAGGAAGACAGCTCTG GAGATGAAGCTGAAAGTCCTTCCAAGGTGAAGAGGTCCAAGGGAATCCATGTGTTCAAAAAGCCCAGTTT CTCTAAAAAGAAGGAAAAAGATTTTAAAATCAAAGAAAAACCCAAAGAAGAAAAGCATAAAGAAGAAAAG CACAAAGAGGAAAAGCATAAAGAAAAGAAATCGAAAGACTTGACAGCAGCTGATGTTGTTAAACAGTGGA AGGAAAAGAAGAAAAAGAAAAAGCCAATTCAGGAGCCAGAGGTGCCTCAGATGGACGCACCGAGTGTCAA GCCCATTTTTGGGGTTCCCTTGGTTGACGCAGTAGAGAGGACCATGATGTACGACGGCGTCCGGCTGCCA GCTGTCTTCCGGGAGTGTGTGGACTACATGGAGAAGCACGGCATGAAGTGTGAAGGCGTCTACCGGGTGT CAGGAATCAAATCAAAGGTAGATGAGCTAAAAGCAGCCTATGACCGAGAGGAGTCTCCAAACTTAGAAGA ATATGAGCCTAACACTGTAGCCAGCTTGCTGAAGCAGTATTTGCGAGACCTTCCAGAGAATTTGCTTACC AAAGAGCTTATGCCCCGGTTTGAAGAGGCTTGTGGGAAGACCACAGAGATGGAGAAAGTGCAGGAATTCC AGCGCTTGCTCCGGGAACTGCCGGAGTGCAATCATCTTCTGCTTTCCTGGCTCATTGTGCACCTGGACCA CGTCATTGCGAAGGAGCTGGAAACGAAGATGAACATCCAGAACATCTCTATAGTGCTGAGCCCCACCGTG CAGATCAGCAATCGGGTCCTGTACGTGCTTTTCACACATGTGCAAGAGCTCTTTGGCACCGTGGTCCTGA AGCAAGTAACAAGACCTCTGCGCTGGTCCAACATGGCCACGATGCCCACACTGCCAGAGACCCAAGCAGG CATCAAGGAGGAGATCAGGAGACAGGAGTTCCTTTTGAATTGTTTACATCGAGATCTGCAGGGCGGGATA AAGGACTTATCTAAAGAAGAAAGATTATGGGAAGTACAGAGGATTCTGACTGCCCTCAAGAGAAAGCTGA GGGAAGCTAAAAGACAAGAGTGTGAAACCAAGATTGCACAGGAGATAGCTAGTCTTTCAAAAGAGGATGT TTCCAAAGAAGAAATGAATGAAAACGAAGAAGTTATAAATATCCTTCTTGCCCAGGAGAATGAAATCCTG ACAGAACAGGAGGAGCTCCTGGCCATGGAGCAGTTCCTGCGTCGTCAGATCGCCTCAGAGAAAGAAGAGA TCGACCGCCTCCGAGCTGAAATTGCGGAGATCCAGAGCCGTCAGCAGCATGGCCGCAGTGAGACGGAGGA GTACTCGTCCGACAGCGAGAGTGAGAGCGAGGACGAGGAGGAGCTGCAGCTCATCCTGGAGGACCTGCAG CGGCAGAACGAGGAGTTAGAAATAAAGAACAATCACTTGAACCAAGCAGTCCATGAGGAGCGGGAGGCCA TCATCGAGCTCCGAGTGCAGCTCCGGCTGCTCCAGATGCAGCGAGCCAAGTCTGAGCAGCAGCCGCAGGA GGAGGAGGAGCCCGAGAGGCGAGGGGGCATAGGCCCACCGCCCTGTGACGGTGTCCTCGAGGTGCGGGTA GCCAAGGAGCAGGCGAAGGCCTCGCCCAGCAAAGACCGGAAGGAGACGCCCATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001198949
Insert Size	1947 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001198949.1, NP_001185878.1
RefSeq Size	3788 bp
RefSeq ORF	1947 bp
Locus ID	19765
UniProt ID	Q62172
Gene Summary	Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an alternate 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.

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