Tlk2 (NM_001294329) Mouse Untagged Clone

CAT#: MC228568

Tlk2 (untagged) - Mouse tousled-like kinase 2 (Arabidopsis) (Tlk2), transcript variant C



  "NM_001294329" in other vectors (1)

CNY 6,270.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 4933403M19Rik; PKU-alpha; PKUalpha; Tlk
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228568 representing NM_001294329
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCAGCGAAATCGGAAAAGGAAAGCCGAGCCATATGACACTAGCCAAGCGTCGAGCAGAACAGCCTC
TGTATGGTTTAGATGGCAGTGCAGCAAAGGAGGCCTCAGAAGAGCAGTCTGCTCTGCCCACCCTCATGTC
AGTGATGTTAGCAAAACCGCGACTTGACACAGAGCAGTTAGCGCCACGGGGAGCTGGCCTCTGCTTCACT
TTCGTCTCTGCTCAACAAAACAGTCCTTCTTCTACGGGTTCTGGCAATACAGAACATTCTTGCAGCTCCC
AAAAACAGATCTCCATCCAGCACAGACAGACCCAGTCTGACCTCACAATAGAAAAAATATCTGCACTAGA
AAACAGTAAGAACTCTGACTTAGAGAAGAAGGAAGGAAGAATAGATGATTTATTAAGAGCCAACTGTGAT
TTGAGACGACAGATAGATGAACAGCAAAAGATGCTAGAGAAATACAAGGAACGATTAAATAGATGTGTCA
CCATGAGCAAGAAGCTCCTTATAGAAAAGTCAAAACAAGAGAAGATGGCGTGCAGAGATAAGAGCATGCA
GGACCGATTGCGATTAGGCCACTTTACTACTGTCCGGCATGGAGCCTCGTTTACTGAGCAGTGGACAGAT
GGTTATGCTTTCCAAAACCTCATCAAGCAACAGGAAAGGATAAATTCACAGAGAGAAGAGATAGAAAGGC
AACGGAAAATGTTAGCAAAACGGAAACCTCCTGCCATGGGTCAGGCCCCTCCTGCAACCAATGAGCAGAA
ACAACGGAAAAGCAAGACTAATGGAGCTGAAAATGAAACGTTAACGTTAGCCGAGTACCATGAACAAGAG
GAAATCTTCAAACTTAGATTAGGTCATCTTAAGAAAGAGGAAGCAGAAATCCAGGCAGAGCTGGAAAGGC
TGGAAAGGGTTAGGAATCTACACATCAGGGAATTAAAAAGGATACATAATGAAGACAATTCGCAGTTTAA
AGACCATCCAACACTAAATGACAGATATTTGTTGTTACATCTTTTGGGTAGAGGAGGTTTCAGTGAAGTT
TACAAGGCATTTGATCTAACGGAGCAAAGATATGTAGCTGTGAAAATTCACCAGTTAAATAAAAACTGGA
GAGATGAGAAAAAGGAGAATTACCACAAGCATGCGTGTAGGGAATACCGGATTCACAAGGAGCTGGACCA
CCCCAGGATAGTGAAGCTGTATGATTACTTTTCACTGGACACTGACTCGTTTTGTACAGTATTAGAATAC
TGTGAAGGGAATGACCTGGACTTCTACCTAAAACAGCACAAATTAATGTCGGAGAAAGAAGCCCGATCCA
TTATTATGCAGATTGTGAATGCTTTAAAGTACTTAAATGAAATAAAACCTCCCATCATACACTATGACCT
CAAACCAGGTAATATCCTTTTAGTAAATGGTACAGCATGTGGAGAGATAAAAATTACAGATTTTGGTCTT
TCCAAGATCATGGATGATGATAGCTACAATTCAGTGGATGGCATGGAGCTGACGTCACAAGGAGCTGGTA
CTTATTGGTATTTACCACCAGAGTGTTTTGTGGTTGGGAAAGAGCCACCAAAGATCTCAAATAAAGTCGA
TGTTTGGTCAGTGGGTGTGATCTTCTACCAGTGTCTTTATGGGAGGAAGCCTTTTGGCCATAACCAGTCC
CAGCAAGATATTCTACAAGAGAATACTATTCTTAAGGCTACTGAAGTACAGTTCCCGCCAAAGCCAGTAG
TAACACCTGAAGCAAAGGCATTTATCAGGAGATGTCTGGCCTATCGAAAGGAAGATCGCATTGATGTGCA
GCAGCTGGCCTGTGACCCCTACTTGTTGCCTCACATCCGAAAGTCAGTCTCCACAAGTAGCCCTGCAGGA
GCTGCTATTGCATCAACCTCTGGGGCATCCAATAACAGTTCTTCGAATTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001294329
Insert Size 1941 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001294329.1, NP_001281258.1
RefSeq Size 5341 bp
RefSeq ORF 1941 bp
Locus ID 24086
Gene Summary Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly (By similarity). Negative regulator of amino acid starvation-induced autophagy (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (C) differs in the 5' UTR and lacks two alternate exons in the coding region compared to variant A; the 5'-most initiation codon, as used in variant A, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the alternate AUG to encode an isoform (C) that has a distinct N-terminus, and is shorter, compared to isoform A. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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