Tlk2 (NM_001294329) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4933403M19Rik; PKU-alpha; PKUalpha; Tlk
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228568 representing NM_001294329 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCAGCGAAATCGGAAAAGGAAAGCCGAGCCATATGACACTAGCCAAGCGTCGAGCAGAACAGCCTC TGTATGGTTTAGATGGCAGTGCAGCAAAGGAGGCCTCAGAAGAGCAGTCTGCTCTGCCCACCCTCATGTC AGTGATGTTAGCAAAACCGCGACTTGACACAGAGCAGTTAGCGCCACGGGGAGCTGGCCTCTGCTTCACT TTCGTCTCTGCTCAACAAAACAGTCCTTCTTCTACGGGTTCTGGCAATACAGAACATTCTTGCAGCTCCC AAAAACAGATCTCCATCCAGCACAGACAGACCCAGTCTGACCTCACAATAGAAAAAATATCTGCACTAGA AAACAGTAAGAACTCTGACTTAGAGAAGAAGGAAGGAAGAATAGATGATTTATTAAGAGCCAACTGTGAT TTGAGACGACAGATAGATGAACAGCAAAAGATGCTAGAGAAATACAAGGAACGATTAAATAGATGTGTCA CCATGAGCAAGAAGCTCCTTATAGAAAAGTCAAAACAAGAGAAGATGGCGTGCAGAGATAAGAGCATGCA GGACCGATTGCGATTAGGCCACTTTACTACTGTCCGGCATGGAGCCTCGTTTACTGAGCAGTGGACAGAT GGTTATGCTTTCCAAAACCTCATCAAGCAACAGGAAAGGATAAATTCACAGAGAGAAGAGATAGAAAGGC AACGGAAAATGTTAGCAAAACGGAAACCTCCTGCCATGGGTCAGGCCCCTCCTGCAACCAATGAGCAGAA ACAACGGAAAAGCAAGACTAATGGAGCTGAAAATGAAACGTTAACGTTAGCCGAGTACCATGAACAAGAG GAAATCTTCAAACTTAGATTAGGTCATCTTAAGAAAGAGGAAGCAGAAATCCAGGCAGAGCTGGAAAGGC TGGAAAGGGTTAGGAATCTACACATCAGGGAATTAAAAAGGATACATAATGAAGACAATTCGCAGTTTAA AGACCATCCAACACTAAATGACAGATATTTGTTGTTACATCTTTTGGGTAGAGGAGGTTTCAGTGAAGTT TACAAGGCATTTGATCTAACGGAGCAAAGATATGTAGCTGTGAAAATTCACCAGTTAAATAAAAACTGGA GAGATGAGAAAAAGGAGAATTACCACAAGCATGCGTGTAGGGAATACCGGATTCACAAGGAGCTGGACCA CCCCAGGATAGTGAAGCTGTATGATTACTTTTCACTGGACACTGACTCGTTTTGTACAGTATTAGAATAC TGTGAAGGGAATGACCTGGACTTCTACCTAAAACAGCACAAATTAATGTCGGAGAAAGAAGCCCGATCCA TTATTATGCAGATTGTGAATGCTTTAAAGTACTTAAATGAAATAAAACCTCCCATCATACACTATGACCT CAAACCAGGTAATATCCTTTTAGTAAATGGTACAGCATGTGGAGAGATAAAAATTACAGATTTTGGTCTT TCCAAGATCATGGATGATGATAGCTACAATTCAGTGGATGGCATGGAGCTGACGTCACAAGGAGCTGGTA CTTATTGGTATTTACCACCAGAGTGTTTTGTGGTTGGGAAAGAGCCACCAAAGATCTCAAATAAAGTCGA TGTTTGGTCAGTGGGTGTGATCTTCTACCAGTGTCTTTATGGGAGGAAGCCTTTTGGCCATAACCAGTCC CAGCAAGATATTCTACAAGAGAATACTATTCTTAAGGCTACTGAAGTACAGTTCCCGCCAAAGCCAGTAG TAACACCTGAAGCAAAGGCATTTATCAGGAGATGTCTGGCCTATCGAAAGGAAGATCGCATTGATGTGCA GCAGCTGGCCTGTGACCCCTACTTGTTGCCTCACATCCGAAAGTCAGTCTCCACAAGTAGCCCTGCAGGA GCTGCTATTGCATCAACCTCTGGGGCATCCAATAACAGTTCTTCGAATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001294329
Insert Size	1941 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001294329.1, NP_001281258.1
RefSeq Size	5341 bp
RefSeq ORF	1941 bp
Locus ID	24086
Gene Summary	Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly (By similarity). Negative regulator of amino acid starvation-induced autophagy (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (C) differs in the 5' UTR and lacks two alternate exons in the coding region compared to variant A; the 5'-most initiation codon, as used in variant A, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the alternate AUG to encode an isoform (C) that has a distinct N-terminus, and is shorter, compared to isoform A. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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