Numb (NM_001272055) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	m-num; Nb
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228559 representing NM_001272055 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAAACTACGGCAAAGCTTCAGGAGAAAGAAAGACGTTTATGTCCCAGAGGCCAGCCGTCCACATC AGTGGCAGACAGATGAAGAAGGAGTCCGCACTGGAAAGTGTAGCTTCCCAGTTAAGTACCTCGGCCACGT AGAAGTTGATGAGTCAAGAGGAATGCACATCTGTGAAGATGCCGTAAAGAGATTGAAAGCTACGGGAAAG AAAGCAGTGAAGGCCGTTCTGTGGGTGTCAGCGGATGGGCTCAGAGTTGTGGACGAGAAAACTAAGGACC TCATAGTTGACCAGACAATAGAAAAAGTTTCCTTCTGTGCCCCAGATAGGAACTTTGACAGAGCCTTTTC TTACATATGTCGTGATGGCACCACTCGGCGATGGATCTGTCATTGTTTCATGGCTGTCAAAGACACGGGG GAAAGACTGAGCCACGCCGTGGGCTGTGCTTTTGCAGCCTGTTTAGAGCGTAAACAGAAGCGGGAGAAGG AGTGTGGCGTCACTGCTACTTTTGATGCCAGTAGAACCACTTTCACAAGAGAAGGATCATTCCGTGTCAC AACTGCCACTGAGCAAGCCGAAAGAGAGGAGATCATGAAACAGTTGCAAGATGCCAAGAAAGCTGAGACA GACAAGACAGTTGTTGGTCCATCAGTGGCTCCTGGCAACACTGCTCCATCCCCATCCTCTCCCACCTCTC CCACTCCGGATGGCACTGCATCTTCAGAGATGAACAATCCCCATGCTATCCCACGCCGGCATGCACCAAT TGAACAGCTTGCTCGTCAAGGCTCTTTCCGGGGATTTCCTGCTCTTAGCCAGAAGATGTCACCCTTTAAA CGCCAGCTGTCCCTACGCATCAATGAGTTGCCTTCCACTATGCAGAGGAAGACCGATTTCCCAATAAAAA ACACAGTGCCCGAGGTGGAAGGAGAGGCCGAGAGCATCAGCTCCTTGTGTTCCCAGATCACCAGTGCCTT CAGCACGCCCTCTGAGGACCCCTTCTCCTCCGCCCCAATGACCAAACCAGTGACATTGGTGGCACCACAG TCTCCTGTGTTACAAGCTAATGGCACTGACTCAGCCTCCCATGTGCTTACTGCTAAGCCAGCCAATACTG CTCTAGCACACGTAGCAATGCCTGTCCGTGAAACCAACCCCTGGGCCCATGTCCCTGATGCTGCTAACAA GGAAATTGCAGCCATACATCCGGGGACTGAGTGGGGTCAGTCTTCTGGTGCTGCCTCTCCAGGTCTCTTC CAGGCTGGTCACAGACGCACTCCCTCTGAAGCTGACCGCTGGTTAGAAGAAGTGTCAAAGAGTGTGCGGG CCCAGCAGCCTCAGGTCTCAGCTGCCCCTCTGCAGCCAGTTCTCCAGCCGCCTCCGCCCGCCGCCATTGC CCCTCCAGCACCTCCTTTCCAAGGACATGCCTTCCTCACGTCCCAGCCTGTGCCCGTGGGTGTGGTCCCA CCCCTACAACCAGCCTTTGTCCCTACCCAGTCCTACCCTGTGGCCAACGGGATGCCCTACCCAGCCTCTA ATGTGCCTGTAGTGGGCATCACCCCATCCCAGATGGTAGCCAATGTGTTTGGCACTGCAGGCCACCCTCA GACAACTCATCCACATCAGTCGCCAAGCCTGGCCAAGCAGCAGACATTCCCTCAATATGAGACAAGTAGT GCTACCACCAGTCCCTTCTTTAAGCCTCCTGCTCAGCACCTCAATGGTTCTGCAGCTTTCAATGGTGTAG ACAATGGTGGGCTAGCCTCAGGAAACAGGCATGCAGAAGTCCCTCCAGGCACCTGCCCAGTGGATCCTTT CGAAGCTCAGTGGGCCGCACTAGAAAGCAAGTCCAAGCAGCGTACAAATCCTTCTCCTACCAACCCTTTC TCCAGTGACTTACAGAAAACATTTGAAATAGAACTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001272055
Insert Size	1929 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001272055.1, NP_001258984.1
RefSeq Size	3349 bp
RefSeq ORF	1929 bp
Locus ID	18222
UniProt ID	Q9QZS3
Gene Summary	This gene encodes a conserved protein that is distributed asymmetrically during cell division in the developing embryo. The encoded protein participates in cell fate decisions by interacting with the Notch receptor. Loss of function of this gene results in severe defects in neural development and loss of viability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (3, also known as p71 or PTBS-PRRL) is shorter than isoform 1.

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