Umod (NM_001278605) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	THP; ureh; Urehd; Urehd1; urehr4; uromu
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228557 representing NM_001278605 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGATCCCTTTGACCTGGATGCTGCTGGTAATGATGGTAACCTCCTGGTTCACTCTGGCTGAAGCCA GTAACTCAACAGAAGCGAGACGGTGTTCTGAATGCCACAACAACGCCACCTGCACGGTGGATGGTGTGGT CACAACGTGCTCCTGCCAGACCGGCTTCACTGGTGATGGGCTGGTGTGTGAGGACATGGATGAGTGTGCT ACCCCATGGACTCACAACTGCTCCAACAGCAGCTGTGTGAACACCCCGGGCTCGTTTAAGTGCTCCTGTC AGGATGGTTTTCGTCTGACGCCTGAGCTGAGCTGCACTGATGTGGATGAGTGCTCAGAGCAGGGGCTCAG TAACTGTCATGCCCTGGCCACCTGTGTCAACACAGAAGGCGACTACTTGTGCGTGTGTCCCGAGGGCTTT ACAGGGGATGGTTGGTACTGTGAGTGCTCCCCAGGCTCCTGTGAGCCAGGACTGGACTGCTTGCCCCAGG GCCCGGATGGAAAGCTGGTGTGTCAAGACCCCTGCAATACATATGAGACCCTGACTGAGTACTGGCGCAG CACAGAGTATGGTGTGGGCTACTCCTGTGACGCGGGTCTGCACGGCTGGTACCGGTTCACAGGCCAGGGT GGCGTTCGCATGGCTGAGACCTGTGTGCCCGTCCTGCGATGCAACACGGCGGCACCCATGTGGCTCAATG GCTCTCATCCCTCGAGTAGTGAAGGCATTGTGAGCCGCACGGCCTGTGCACACTGGAGCGACCAATGCTG CCGGTGGTCCACAGAGATCCAGGTGAAGGCTTGCCCAGGTGGCTTCTATATTTACAACTTGACAGCGCCC CCTGAGTGCAATCTGGCTTACTGCACCGATCCTAGTTCCGTGGAGGGGACTTGCGAAGAATGCAGGGTAG ATGAAGATTGCATATCGGATAACGGCAGATGGCGCTGCCAGTGTAAACAGGACTCCAACATCACAGATGT CTCCCAATTGGAGTACAGGCTGGAGTGTGGGGCCAATGACATCAAGATGTCCCTCAGAAAGTGCCAGCTA CAGAGTTTGGGCTTTATGAATGTCTTCATGTACCTGAATGACAGACAATGCTCAGGCTTCAGTGAGAGTG ATGAACGAGACTGGATGTCCATAGTGACCCCTGCCAGGAATGGTCCCTGTGGGACAGTATTGAGGAGAAA CGAAACCCATGCCACCTACAGCAACACCCTCTACCTGGCAAATGCGATCATCATTCGGGACATCATCATA AGAATGAACTTTGAATGCTCTTACCCTCTGGACATGAAAGTCAGCCTGAAGACCTCCCTACAGCCCATGG TCAGTGCCCTGAACATCAGCTTGGGTGGGACAGGCAAGTTCACCGTGCGGATGGCATTGTTCCAGAGCCC TACCTACACACAGCCCCACCAAGGTCCTTCTGTGATGCTGTCCACTGAGGCTTTTCTGTATGTGGGCACC ATGCTGGATGGGGGTGACTTGTCCCGGTTTGTACTGCTAATGACCAACTGCTATGCCACACCCAGTAGCA ACTCCACAGACCCTGTGAAATACTTCATTATCCAGGACAGTTGTCCACGTACAGAAGATACAACCATTCA GGTGACAGAGAATGGCGAGTCATCTCAGGCCCGATTTTCTGTTCAGATGTTCCGGTTTGCAGGAAACTAC GACCTTGTCTACCTTCACTGCGAGGTGTACCTATGTGACTCTACGAGTGAACAGTGTAAACCTACCTGCT CTGGTACTAGATTTCGAAGTGGGAACTTCATAGATCAGACCCGTGTCCTGAACTTGGGTCCCATAACACG ACAAGGTGTCCAGGCCTCAGTGTCCAAGGCTGCTTCCAGCAACTTGAGGCTCCTGAGCATCTGGCTGCTG TTGTTTCCCTCAGCCACTTTGATCTTCATGGTTCAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001278605
Insert Size	1929 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278605.1, NP_001265534.1
RefSeq Size	2581 bp
RefSeq ORF	1929 bp
Locus ID	22242
UniProt ID	Q91X17
Gene Summary	This gene encodes a glycoprotein that is the most abundant protein in mammalian urine under physiological conditions. It is synthesized in the kidney as a glycosyl-phosphatidylinositol anchored protein and released into urine as a soluble form by proteolytic cleavage. It is thought to regulate water and salt balance in the thick ascending limb of Henle and to protect against urinary tract infection and calcium oxalate crystal formation. In mouse deficiency of this gene is associated with increased susceptibility to bacterial infections and formation of calcium crystals in kidneys. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.

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