Gga3 (NM_001252067) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C230037M19Rik; mKIAA0154
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228553 representing NM_001252067 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGCGGAAGGGGAGAGCCTGGAGTCCTGGCTGAATAAAGCTACCAATCCTTCCAACCGCCAGG AAGACTGGGAATATATCATTGGTTTCTGTGATCAGATCAACAAAGAGCTTGAAGGGCCCCAGATTGCTGT GCGACTCCTGGCCCACAAGATCCAGTCTCCACAGGAATGGGAGGCAGTGCAGGCCCTCACGGTGCTGGAG GCGTGTATGAAGAACTGTGGCAGGAGACTTCATAATGAAGTGGGGAAGTTCCGGTTTTTGAATGAATTAA TCAAAGTCGTCTCTCCAAAGTACTTGGGAGACAGGGTGTCTGAAAAGGTGAAGACTAAGGTCATCGAGTT GCTTTTTAGCTGGACACTGGCCTTGCCGGAAGAAGCAAAGATCAAAGATGCCTACCATATGTTGAAGAGA CAAGGCATAGTGCAGTCGGACCCCCCCATCCCCATGGATAGAACGCTGATCCCCTCTCCCCCACCTCGGC CCAAAAACCCCGTTTTTGATGATGAGGAGAAATCCAAGCTTTTAGCCAGGCTGCTGAAGAGCAAGAACCC AGATGACCTGCAAGAGGCCAACAGACTCATCAAGTCCATGGTGAAGGAAGATGAGGCTCGCATCCAGAAA GTGACCAAGCGTCTACACACACTGGAGGAGGTTAACAACAATGTCAAGCTGCTCCACGAGATGCTGCTTC ACTACAGCCAAGAGTACTCGTCAGACGCCGACAAGGAGCTCATGAAGGAGCTCTTTGATCGTTGTGAGAA TAAGAGGCGGACGCTGTTTAAACTAGCCAGCGAGACAGAGGACAATGACAACAGTTTGGGGGACATCCTG CAGGCCAGTGACAACCTGTCCAGGGTCATCAACTCTTACAAAACTATTATTGAAGGGCAGATCGTCAATG GTGAGGTGACCACCTCAACCATGCCTGACTCTGAAGGCCTTACTGACCCCGCCCCCACTGCTCCCAAAGA GTCACCTGGAAGTAGTCAGTGGCACCTGTTCCAGAATGAACCACCATCAGATCTGGACTTCTTCAGTCCC AGGCCAGTGCCTGCGGCCTCCTGCCCCTCAGATGGACCCCAGCTCCCTCCCCCAGTTTCTACCTCAAGTA TGTCCCAAGCTCCACTGCCTGCTGCCTTCCCAGCTCCTGTGGTCCCAGCCAGTGCACCAACCCACAGCAC TGGCTCCTTCATGTTCTCTTCTGGACCTGCCCCAGCCTTGGCTCCAAAGGCTGAGCCCAAAGGTCCAGAA TACCCCAGCTCCAGCACCTCGCACCGCCTAGATGCCCTTGATCAGCTTCTGGAAGAGGCCAAAGTAACCT CAGGCTTGGTGAAACCTGTTTCTTGCTTCTCTCCTGGGCCCACTGCCTCCCCGCTGCTGCCTGCCTCTGC CCCAGCCAGACCTCTCCTGCCCTTCTCCACTGGGCCCGGAAGCCCTCTCTTCCAGTCCCAAGGCAGCCCT CAGAAAGGCCCAGAGCTCTCCTTGGCCAGTGTCCATGTGCCCCTGGAATCGATCAAGCCTAGCAGTGCCC TTCCCGTGACAGCCTATGATAAAAATGGCTTCCGCATCCTTTTCCACTTTGCTAAGGAGTGTCCGCCAGG ACGGCCTGATGTGCTGGTGGTGGTGGTTTCCATGCTGAATACGGCTCCGTTGCCAGTCAAGAGCATAGTG CTGCAGGCTGCAGTGCCCAAGTCAATGAAAGTGAAGTTGCAGCCACCCTCTGGGACAGAACTTTCCCCAT TTAGTCCTATCCAGCCACCTGCAGCCATCACCCAGGTCATGCTGCTGGCCAATCCAATGAAGGAAAAGGT GCGGCTTCGATATAAACTGACCTTTGCTTTGGGGGAGCAGCTGAGCACAGAACTGGGTGAAGTGGACCAG TTTCCTCCTGTGGAGCAGTGGGGGAACCTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252067
Insert Size	1923 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252067.1, NP_001238996.1
RefSeq Size	3469 bp
RefSeq ORF	1923 bp
Locus ID	260302
Gene Summary	Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.

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