Capn5 (NM_001301250) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	nCL-3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228548 representing NM_001301250 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTCCTGCGCGAAGGCCTATGAGGACCAGAACTACTCGGCGCTGAAGCGGGCCTGCCTGCGCAAGA AGGTGCTGTTCGAGGATCCCCTCTTCCCTGCCACCGACGACTCCCTTTACTATAAGGGCACCCCAGGGCC CACAGTCAGGTGGAAGCGGCCTAAGGATATCTGCGACGATCCCCGGCTCTTCGTAGATGGCATCAGCTCC CATGACCTGCACCAGGGCCAGGTGGGCAACTGCTGGTTTGTGGCTGCCTGCTCATCACTGGCCTCCCGAG AGTCACTCTGGCAGAAGGTCATCCCAGACTGGAAGGAGCAGGAATGGAACCCCGAGAAGCCTGACAGCTA TGCTGGCATCTTCCACTTCAACTTCTGGCGCTTTGGGGAGTGGGTGGACGTAATCGTCGATGACCGGCTG CCCACAGTCAACAACCAGCTCATTTACTGCCATTCCAACTCCAAAAATGAGTTCTGGTGTGCCCTGGTGG AGAAGGCCTATGCCAAGCTGGCCGGCTGTTACCAGGCCCTGGACGGAGGCAACACGGCCGATGCATTGGT GGATTTCACAGGTGGTGTTTCTGAACCCATTGACCTGACCGAGGGGGACTTGGCCACTGACGAGGCTAAG AGGAATCAGCTCTTTGAGCGAGTGCTGAAGGTGCACAGCAGAGGCGGGCTCATCAGTGCCTCCATCAAGG CTGTGACAGCAGCTGACATGGAGGCCCGCCTGGCATGTGGCCTGGTGAAGGGCCATGCATACGCTGTCAC CGATGTGCGCAAGGTGCGCCTGGGCCATGGCCTGCTGGCCTTCTTCAAGTCAGAGAAGCTTGATATGATC CGTCTGAGGAACCCCTGGGGCGAGCGGGAGTGGACGGGGCCCTGGAGTGACACGTCAGAGGAATGGCAGA AAGTGAGCAAGAGTGAGAGGGAGAAGATGGGCGTGACCGTGCAGGATGATGGGGAATTCTGGATGACCTT TGAGGACATGTGCCGGTACTTTACTGACATCATTAAATGCCGCCTGATTAACACGTCCTACCTGAGCATC CATAAGACATGGGAGGAGGCCCGGCTGCATGGTGCCTGGACGAGACATGAGGACCCACAGCAGAACCGCA GTGGAGGCTGCATCAACCACAAGGACACTTTCTTCCAGAACCCACAGTACGTATTTGAAGTCAAGAAGCC AGAAGATGAAGTGTTGATCAGTATCCAGCAGCGGCCGAAGCGCTCAACTCGCCGGGAGGGCAAAGGCGAG AATCTGGCCATTGGCTTCGACATCTATAAGGTGGAAGAGAACCGCCAATACCGTATGCACAGCCTACAGC ATAAGGCCGCCAGCTCCATCTACATCAATTCCCGCAGCGTTTTTTTGAGGACAGAGCTGCCCGAGGGCCG CTACGTTATCATCCCTACCACCTTTGAGCCAGGCCACACTGGCGAGTTCCTGCTCCGAGTCTTCACAGAT GTCCCCTCCAACTGCCGGGAACTACGCCTGGATGAGCCCCCTCGGACCTGTTGGAGTTCCCTCTGTGGCT ACCCTCAGCAGGTGGCCCAGGTACATGTCCTGGGGGCTGCTGGCCTCAAGGACTCCCCAACAGGAGCAAA CTCATATGTGATCATCAAGTGTGAGGGCGAAAAGGTTCGCTCAGCTGTGCAGAGAGGGACCTCGACACCA GAGTACAATGTAAAAGGCATCTTCTATCGCAAGAAGCTGGCTCAGCCTATCACCGTGCAGGTTTGGAATC ACCGAGTCCTGAAGGATGAATTCCTGGGCCAGGTGCACCTGAAGACTGCCCCGGATGACCTGCAGGACCT CCACACCCTCCATCTCCAGGACCGCAGTAGCCGGCAGCCCAGTGACCTGCCAGGCATTGTAGCTGTGCGA GTCCTCTGCAGTGCCTCTCTCACGGCTGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301250
Insert Size	1923 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301250.1, NP_001288179.1
RefSeq Size	4351 bp
RefSeq ORF	1923 bp
Locus ID	12337
UniProt ID	O08688
Gene Summary	The protein encoded by this gene is a member of the calpain family of proteins. Unlike many members of the calpain gene family, this gene lacks a calmodulin-like domain, required for calcium binding. Mouse models for Huntington's disease displayed increased levels of the protein encoded by this gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR and represents use of an alternate promoter, compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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