Scnn1b (NM_001272023) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228543 representing NM_001272023 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGTGAAGAAGTACCTCCTGAAGTGCCTGCACCGGCTGCAGAAGGGCCCAGGCTACACCTACAAGG AGCTGCTAGTGTGGTACTGCAATAACACCAACACCCACGGCCCCAAACGCATCATCTGTGAGGGGCCCAA GAAGAAGGCCATGTGGTTCCTGCTTACGCTGCTCTTCGCCTGCCTGGTGTGCTGGCAGTGGGGAGTCTTC ATCCAGACCTACCTGAGCTGGGAGGTCAGCGTCTCGCTCTCCATGGGCTTCAAGACGATGAACTTCCCGG CGGTCACCGTCTGCAATTCCAGCCCCTTCCAGTACTCCAAGGTCAAGCACTTGCTCAAGGACTTGGATGA GCTAATGGAGGCAGTCCTGGAAAAGATTCTGGCTCCAGAGGCCAGCCACAGCAACACCACCAGGACCCTG AACTTTACCATCTGGAACCACACACCCCTGGTCCTTATTGATGAGCGGAACCCTGACCACCCGGTGGTTC TCAATTTGTTTGGGGACAGCCACAACAGCAGCAACCCGGCTCCAGGAAGCACCTGCAATGCCCAGGGATG CAAAGTGGCCATGAGGCTGTGCAGTGCCAATGGAACTGTGTGTACCTTGCGGAACTTCACCAGTGCCACC CAGGCCGTAACTGAGTGGTACATCCTGCAGGCCACCAACATCTTCTCACAAGTGCTTCCTCAGGACCTGG TGGGGATGGGCTATGCCCCTGATCGCATAATCCTAGCTTGCCTGTTTGGAACAGAGCCCTGCAGTCATCG GAACTTCACGCCTATCTTCTACCCTGATTATGGCAACTGCTACATCTTCAACTGGGGCATGACAGAGGAG ACACTTCCTTCTGCCAACCCTGGGACTGAATTTGGTCTCAAGTTGATCCTGGACATCGGTCAGGAGGACT ATGTCCCCTTCCTTGCGTCCACAGCAGGGGCTAGGCTGATGCTTCATGAGCAGAGGACGTACCCCTTCAT TAGAGAGGAGGGCATCTATGCCATGGCAGGAACTGAGACGTCTATTGGGGTGCTGGTGGACAAGCTGCAA CGCAAGGGGGAGCCCTACAGTCCCTGCACCATGAACGGCTCCGATGTTGCCATAAAGAACCTCTACAGTG TCTACAACACCACCTATTCCATCCAGGCCTGTCTTCATTCCTGTTTCCAAGACCACATGATCCGTAACTG CAGTTGTGGCCACTACTTATACCCACTGCCTGAAGGAGAGAAATACTGCAACAACAGGGACTTCCCAGAC TGGGCCTATTGCTATCTAAACCTGCAGATGAGTGTGACCCAGAGAGAGACCTGCCTCAGCATGTGCAAGG AGTCCTGCAATGACACCCAGTATAAGATGACCATCTCCATGGCTGACTGGCCATCTGAGGCCTCTGAGGA TTGGATCTTACATGTCCTGTCTCAGGAGCGGGACCAGAGCTCGAATATCACCCTGAGCAGGAAGGGTATT GTCAAGCTCAATATCTACTTCCAAGAGTTCAACTACCGTACCATTGAGGAATCTCCAGCCAACAATATTG TGTGGCTGCTCTCTAACCTGGGGGGCCAGTTTGGCTTCTGGATGGGGGGCTCGGTGCTGTGCCTCATTGA GTTTGGGGAGATCATTATCGACTTCATTTGGATCACCATCATCAAGCTGGTGGCCTCCTGCAAAGGCCTG CGCAGGAGGCGGCCACAGGCACCCTACACTGGCCCACCGCCCACTGTGGCTGAGCTGGTAGAGGCCCACA CCAACTTTGGCTTCCAGCCTGACACAACCAGCTGCAGGCCCCACGGCGAGGTCTACCCTGACCAACAGAC TCTGCCCATCCCGGGGACTCCACCCCCCAACTATGACTCCCTGAGGCTGCAGCCGCTGGACACCATGGAG TCGGACAGTGAGGTGGAGGCCATCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001272023
Insert Size	1917 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001272023.1, NP_001258952.1
RefSeq Size	2697 bp
RefSeq ORF	1917 bp
Locus ID	20277
UniProt ID	Q9WU38
Gene Summary	Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.

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