Ints9 (NM_001253731) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	BC028953; D14Ertd231e
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228536 representing NM_001253731 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGACTGTGGACTGGACATGACTTCCACCCTCAATTTCCTCCCTTTGCCTCTTGTTCAAAGCCCCA GGCTATCTAATCTTCCCGGATGGTCTCTAAAAGACGGAAATGCTTTTTTGGACAAGGAGCTAAAAGAGTG CTCCGGTCATGTATTTGTGGATTCTGTGCCTGAATTCTGCTTACCAGAGACCGAGCTAATAGACCTGTCT ACAGTCGATGTGATCCTCATCTCTAACTACCACTGCATGATGGCGCTGCCCTACATCACCGAGCACACAG GATTCACAGGCACAGTGTATGCCACAGAACCCACCATGCAGATCGGCAGGCTTCTCATGGAAGAGCTGGT GAATTTCATCGAAAGAGTGCCAAAGGCCCAGTCTGCCTCCTTGTGGAAGAATAAGGATATACAGCGGCTG CTGCCTTCTCCCCTCAAGGATGCAGTGGAAGTGTCAACCTGGAGAAGATGCTACACGATGCAGGAGGTGA ACTCGGCCCTCAGTAAAATCCAGCTGGTGGGATATTCCCAGAAAATCGAGCTGTTTGGTGCTGTCCAGGT GACCCCTCTGAGCTCTGGCTATGCCCTTGGAAGCTCCAACTGGATTATCCAGTCACATTATGAGAAAGTA TCTTACGTCTCTGGATCCTCCTTGCTTACCACACACCCTCAGCCCATGGACCAAGCTTCTCTCAAAAACA GTGACGTTCTGATTCTCACAGGGCTGACACAGATCCCTACTGCAAACCCGGACGGCATGGTGGGCGAGTT CTGCAGCAACCTCGCTCTGACAGTTCGGAATGGAGGAAACGTGTTGGTCCCTTGCTATCCTTCAGGCGTG ATCTATGACCTCCTGGAGTGCTTATATCAATACATTGACTCCGCTGGCCTCTCCAACATCCCTTTCTACT TCATCTCCCCCGTTGCCAACAGTTCACTCGAGTTTTCCCAGATTTTTGCTGAGTGGCTTTGTCACAACAA ACAGAGTAAAGTTTACCTCCCAGAACCACCTTTCCCACATGCAGAGCTCATTCAGACGAATAAGCTGAAG CACTACCGCAGCATCCACGGTGACTTCAGCAATGACTTCAGACAGCCGTGTGTGCTGTTCACCGGACATC CTTCCCTCCGCTTTGGGGACGTCGTCCACTTCATGGAGCTCTGGGGGAAGTCCAGCCTCAACACCATCAT ATTCACAGAACCCGATTTCTCCTACCTGGAGGCCTTGGCTCCTTACCAGCCGCTGGCCATGAAGTGCATC TACTGCCCAATTGACACTCGACTGAATTTCATCCAGGTGTCAAAGCTGCTTAAAGAAGTACAGCCCCTGC ACGTGGTCTGTCCTGAGCAGTACACCCAGCCACCGCCAGCCCAGGCCCACAGGATGGACCTCATGATCGA CTGCCAGCCACCAGCCATGTCCTATCGACGGGCTGAGGTCCTTGCCCTGCCCTTCAAACGACGCTATGAG AAAATTGAAATCATGCCAGAGCTGGCAGACTCCCTGGTGCCCATGGAAATCAAGCCTGGCATCTCCTTGG CGACTGTCTCAGCTGTGCTGCATACGAAGGATAACAAACATGTGCTTCAGCCCCCTCCCAAGCCCACCCA GCCCACCAGCAGTAAGAAGAGGAAGCGGGTGAACGAGGACATTCCTGACTGCAAAGTACTGAAGCCTCTG CTGAGTGGCTCCATCCCCGTAGAGCAGTTTGTGCAGACCCTGGAGAAGCATGGCTTCAGTGACATTAAGG TGGAAGACACAGCCAAGGGACATATTGTCCTGCTGCAGGAAGCCGAGACACTCATCCAGATTGAAGAAGA CTCCACTCACATCATCTGTGACAATGACGAGACACTGAGGGTCCGACTGCGGGACCTTGTCCTCAGGTTC CTGCAGAAGTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253731
Insert Size	1905 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253731.1, NP_001240660.1
RefSeq Size	2727 bp
RefSeq ORF	1905 bp
Locus ID	210925
UniProt ID	Q8K114
Gene Summary	Component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an alternate splice site in the 5' region, which results in a downstream AUG start codon, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1.

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