Grip1 (NM_001277295) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4931400F03Rik; eb; GRIP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228533 representing NM_001277295 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGCAAAACGAGCTGAAAGGAAGGAGATGAAGAGACCCAATAGTTTTCACCTTCCTTTTCGTCCAT CTTTAAGAAAAGGACAGAAGAAAAACGCAGCTCATGTGTCTTTAGCCTCCAGCACTGTGGGGTTGGCTGG CCAGGTCGTTCACACTGAAACCACAGAGGTTGTGCTGACGGCTGACCCTGTCACGGGCTTCGGAATCCAA CTGCAGGGCAGCGTGTTTGCCACAGAGACGCTCTCCTCTCCGCCTCTGATTTCCTATATTGAAGCTGACA GCCCAGCAGAGAGATGTGGTGTGCTACAGATTGGAGACAGAGTCATGGCCATTAATGGAATCCCAACAGA AGACAGCACCTTCGAGGAAGCCAATCAACTCCTGAGAGACTCTTCCATCACGAGCAAAGTCACACTAGAA ATCGAGTTTGATGTTGCAGAGTCTGTCATCCCAAGTAGTGGAACATTTCATGTAAAACTGCCCAAGAAGC ACAGCGTGGAACTTGGAATAACCATCAGTTCGCCATCCAGTAGAAAACCGGGGGACCCCCTTGTCATTTC AGATATCAAGAAAGGCAGTGTGGCACACAGAACCGGAACTCTGGAACTGGGAGACAAACTGCTTGCGATA GATAACATCCGGCTGGATAACTGTTCCATGGAAGATGCGGTCCAGATCCTCCAGCAGTGTGAAGACCTGG TGAAGCTCAAAATCCGCAAAGATGAAGATAACTCAGACGAGCAAGAGAGTTCCGGAGCGATTATTTACAC GGTGGAGCTGAAGCGCTATGGGGGGCCCCTTGGCATCACAATTTCTGGAACTGAAGAGCCGTTTGATCCT ATTATCATCTCGAGCCTCACTAAAGGGGGATTAGCTGAAAGGACTGGAGCGATCCACATCGGAGATAGAA TCCTAGCCATCAATAGCAGCAGCTTGAAGGGGAAGCCTCTGAGTGAAGCCATCCACTTGCTCCAGATGGC AGGAGAGACTGTCACCCTGAAAATTAAGAAACAGACAGATGGCTCAACTTTTCAGACTTCAGGATACAAT TACAACACCTATGATTGGAGGAGTCCAAAGCAAAGAACCAGCCTGTCCCCAGTCCCCAAGCCTCGAAGCC AGACGTACCCAGATGTGGGCCTGAGTAATGAAGACTGGGATCGATCCACAGCCAGTGGCTTTGTAGGGGC TTCTGACAGTGCAGATGCTGAACAAGAGGAAAACTTCTGGTCTCAAGCATTGGAGGACCTGGAGACCTGC GGCCAGTCGGGGATCCTGAGAGAGCTTGAGGCAACAATCATGTCGGGGAGTACTATGAGTTTGAATCATG AGGCTCCAATGGCTCGCAGTCAGCTGGGGCGACAGGCCAGCTTCCAGGAACGGAGCAGTTCACGGCCACA CTATAGCCAAACAACTCGCAGCAACACCCTGCCCTCAGACGTGGGCAGAAAGTCTGTAACCCTGCGGAAA ATGAAGCAAGAAATAAAGGAGATCATGTCCCCAACTCCGGTGGAGCTACACAAGGTGACCTTATACAAGG ACTCTGGCATGGAGGACTTCGGGTTCAGTGTGGCAGATGGCCTGCTGGAGAAAGGCGTGTATGTCAAAAA TATCCGCCCAGCTGGGCCAGGTGATGTTGGGGGCTTGAAGCCCTACGACAGGCTCTTACAGGTAAATCAC GTGCGGACGAGAGACTTTGACTGCTGCCTGGTGGTGCCTCTCATAGCTGAATCTGGCAACAAGCTGGACC TGGTTATTAGCAGAAATCCACTGGCCTCCCAGAAGTCGATAGAACAGCCGGCTCTGCCCAGCGACTGGAG CGAACAGAACAGCGCTTTCTTCCAGCAACCCAGCCACGGTGGTAATCTAGAGACACGAGAACCCACTAAC ACACTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277295
Insert Size	1899 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277295.1, NP_001264224.1
RefSeq Size	3758 bp
RefSeq ORF	1899 bp
Locus ID	74053
UniProt ID	Q925T6
Gene Summary	This gene encodes a protein containing multiple PDZ (post synaptic density protein, Drosophila disc large tumor suppressor, and zonula occludens-1 protein) domains. The encoded protein acts as a mediator between cytoskeletal and membrane proteins, particularly in neuronal cells, and facilitates complex formation at the cell membrane. Mutation of this gene can cause embryonic lethality resulting from defects of the dermo-epidermal junction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013] Transcript Variant: This variant (7) contains multiple differences compared to variant 1, including the lack of multiple exons. It represents use of an alternate promoter and initiates translation at an alternate start codon. The encoded isoform (7, also known as tau) is shorter and has a distinct N-terminus, compared to isoform 1. This isoform is localized to the nucleus (PMID: 15507123). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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