Mid1 (NM_001290506) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	61B3-R; DXHXS1141; Fxy; Trim18
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228523 representing NM_001290506 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAACACTGGAGTCGGAGCTGACCTGTCCTATTTGTCTGGAGCTTTTTGAGGACCCTCTCCTGCTTC CCTGCGCACATAGCCTCTGCTTCAACTGTGCCCACCGAATCCTGGTCTCTCACTGTGCCACCAACGAGCC TGTGGAGTCCATCAACGCCTTCCAGTGCCCCACCTGCCGGCATGTCATCACGCTCAGCCAGCGAGGTCTA GACGGGCTCAAGCGCAACGTCACCCTCCAAAACATCATTGACAGATTTCAGAAAGCATCTGTGAGCGGGC CCAACTCTCCCAGTGAGACCCGCAGGGAACGGGCCTTTGACGCCAACACCATGTCCTCTGCCGAGAAGGT TCTCTGCCAGTTCTGTGACCAGGATCCTGCCCAGGATGCTGTGAAGACTTGCGTTACTTGTGAAGTGTCC TACTGTGATGAGTGCCTGAAAGCCACTCATCCGAACAAGAAGCCCTTTACAGGCCATCGTCTGATTGAGC CAATCCCGGACTCGCACATCCGGGGGCTGATGTGCCTGGAGCACGAGGATGAGAAGCAAAACTTGGAGAG TAATCTCACCAATCTTATTAAGAGAAACACAGAACTGGAGACTCTTTTGGCTAAACTCATCCAAACTTGT CAACATGTTGAAGTCAATGCATCCCGTCAAGAAGCCAAACTGACAGAAGAATGTGATCTTCTCATTGAAA TCATTCAGCAACGAAGACAAATTATTGGAACAAAGATTAAAGAAGGCAAGGTGATCAGGCTCCGCAAGTT AGCTCAGCAGATTGCAAACTGTAAACAGTGCCTTGAGAGGTCTGCATCGCTCATCTCGCAAGCGGAGCAC TCGCTGAAGGAAAATGACCACGCCCGTTTTCTACAGACAGCAAAGAATATCACTGAGAGAGTCTCCATGG CAACTGCATCCTCCCAGGTCCTAATTCCCGAAATCAACCTCAATGACACGTTTGACACTTTTGCCTTGGA TTTTTCCCGGGAGAAGAAACTGCTAGAATGTCTGGATTACCTAACAGCTCCCAACCCTCCCGCGATTAGA GAAGAGCTCTGCACCGCTTCCTACGACACCATCACCGTCCACTGGACCTCAGAGGACGAGTTCAGCGTGG TCTCCTACGAGCTCCAGTACACCATATTCACCGGACAAGCCAATGTTGTCAGTCTGTGTAACTCGGCGGA CAGCTGGATGATCGTGCCCAACATCAAGCAGAACCACTACACCGTGCACGGCCTGCAAAGTGGCACCAAG TATATCTTCACGGTGAAGGCCATCAACCAGGCGGGCAGCCGTAGCAGCGAGCCCGGAAAGCTGAAGACCA ACAGTCAGCCGTTTAGACTGGATCCCAAATCGGCTCATCGCAAGCTGAAGGTGTCCCACGACAACCTGAC TGTCGAGCGCGACGAGTCGTCCTCTAAGAAGAGTCACGCGCCGGAGCGCTTCGCTGGTCAGGGGAGCTAC GGAGTGGCTGGCAACGTGTTCATCGACAGCGGCCGTCACTACTGGGAAGTGGTCACCAGCGGAAGCACAT GGTACGCCATCGGCCTGGCGTACAGATCGGCGCCGAAACACGAGTGGATCGGGAAGAACGCGGCGTCCTG GGCCCTCTGCCGCTGCCACAACCACTGGGCGGTGCGACACGACGGCAAGGAGACCCCCATCGCGCCGGCC CCTCACCTCAGGCGCGTCGGCGTCCTGCTGGACTACGACAACGGCTCCATCGCCTTCTACGACGCTCTGA GCTCCGTCCACCTCCACACCTTCCACGCGGCGCTCGCGCAGCCCGTGTGCCCCACCTTCACCGTGTGGAA CAAGTGTCTGACCATCGTCACGGGTCTGCCCATCCCGGACCATCTGGACTGTACGGAGCAGCGACCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290506
Insert Size	1890 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290506.1, NP_001277435.1
RefSeq Size	3824 bp
RefSeq ORF	1890 bp
Locus ID	17318
UniProt ID	O70583
Gene Summary	Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) differs in the 5' UTR and uses two alternate in-frame splice sites, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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