Itk (NM_001281965) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Emt; Tcsk; Tsk
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228512 representing NM_001281965 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAACTTCATCCTCCTGGAAGAACAGCTGATCAAGAAGTCCCAACAGAAGAGAAGGACTTCTCCCT CGAATTTTAAAGTTCGTTTCTTTGTCTTAACGAAAGCCAGCCTGGCCTACTTTGAGGACCGCCACGGGAA GAAGCGCACGTTGAAGGGCTCCATTGAACTCTCCAGAATCAAGTGTGTGGAGATTGTCAAGAGTGACATT AGCATCCCGTGCCACTATAAATACCCTTTTCAGACTCTTGTGTACTTACAGGTCGTGCATGACAACTATC TCCTGTATGTGTTTGCTCCAGACTGTGAGAGTCGGCAGCGCTGGGTGCTGACCCTTAAAGAAGAAACGAG GAATAACAACAGCCTGGTATCCAAGTATCACCCTAATTTCTGGATGGATGGGCGGTGGAGGTGCTGCTCC CAGCTGGAGAAGCCTGCTGTAGGCTGTGCTCCCTACGACCCATCCAAGAATGCTTCAAAGAAGCCTCTTC CTCCTACTCCTGAAGACAACAGGCGGTCATTTCAGGAACCTGAAGAAACCCTGGTCATTGCCTTGTACGA CTACCAAACCAACGACCCTCAGGAGCTCGCACTGCGGTGTGATGAAGAGTACTACCTGCTGGACAGCTCC GAGATCCACTGGTGGAGGGTTCAAGACAAAAATGGGCATGAAGGATATGCACCAAGCAGTTACCTGGTAG AAAAATCTCCAAATAACCTTGAAACCTATGAGTGGTACAATAAAAGCATCAGCCGCGACAAAGCTGAAAA ACTTCTTTTGGACACAGGTAAAGAAGGAGCTTTCATGGTCCGAGATTCCAGGACACCCGGGACATACACA GTCTCTGTTTTCACCAAGGCCATCATAAGTGAGAACCCCTGTATAAAACATTATCACATCAAAGAAACAA ATGACAGCCCCAAGCGCTACTACGTGGCTGAGAAGTATGTGTTTGACTCCATCCCTCTCCTCATCCAGTA TCACCAGTACAATGGAGGAGGTTTGGTCACTCGACTCCGCTATCCAGTTTGCTCCTGGAGACAAAAAGCC CCTGTCACAGCAGGGCTAAGATATGGGAAGTGGGTGATCCAACCCTCAGAGCTAACGTTCGTGCAGGAGA TTGGCAGCGGGCAGTTTGGGCTGGTGCATCTCGGCTACTGGCTCAACAAGGACAAGGTGGCCATCAAGAC CATTCAGGAAGGGGCGATGTCAGAAGAAGACTTTATCGAGGAGGCGGAAGTCATGATGAAACTCTCTCAC CCCAAACTGGTGCAGCTCTATGGGGTGTGCCTGGAGCAAGCCCCCATCTGCCTGGTGTTTGAGTTCATGG AGCACGGCTGCCTGTCGGATTACCTTCGAAGTCAGCGGGGTCTCTTTGCTGCGGAGACCCTGCTGGGCAT GTGCCTGGATGTGTGTGAGGGCATGGCCTACCTGGAAAAAGCTTGTGTCATCCACAGAGACCTGGCAGCC AGAAACTGTTTGGTGGGAGAAAACCAGGTCATCAAGGTGTCCGACTTTGGGATGACAAGATTTGTCCTTG ATGATCAATATACCAGCTCCACGGGCACCAAATTCCCAGTGAAGTGGGCATCCCCAGAGGTGTTCTCCTT TAGTCGCTATAGCAGCAAGTCAGATGTGTGGTCGTTTGGTGTACTGATGTGGGAAGTCTTCAGTGAAGGC AAAATCCCATACGAAAACCGAAGCAATTCCGAGGTCGTGGAAGATATCAGCACTGGCTTTCGGTTATACA AGCCCCGCCTGGCCTCCTGTCATGTCTACCAGATCATGAATCATTGCTGGAAAGAGAAACCAGAAGACCG GCCACCCTTCTCCCAGCTGTTAAGCCAGCTGGCTGAAATCGCAGAAGCTGGGCTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001281965
Insert Size	1878 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281965.1, NP_001268894.1
RefSeq Size	4292 bp
RefSeq ORF	1878 bp
Locus ID	16428
UniProt ID	Q03526
Gene Summary	Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Phosphorylates TBX21 at 'Tyr-525' and mediates its interaction with GATA3 (PubMed:15662016).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

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