Itk (NM_001281965) Mouse Untagged Clone

CAT#: MC228512

Itk (untagged) - Mouse IL2 inducible T cell kinase (Itk), transcript variant 1



  "NM_001281965" in other vectors (1)

CNY 6,080.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Emt; Tcsk; Tsk
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228512 representing NM_001281965
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAACTTCATCCTCCTGGAAGAACAGCTGATCAAGAAGTCCCAACAGAAGAGAAGGACTTCTCCCT
CGAATTTTAAAGTTCGTTTCTTTGTCTTAACGAAAGCCAGCCTGGCCTACTTTGAGGACCGCCACGGGAA
GAAGCGCACGTTGAAGGGCTCCATTGAACTCTCCAGAATCAAGTGTGTGGAGATTGTCAAGAGTGACATT
AGCATCCCGTGCCACTATAAATACCCTTTTCAGACTCTTGTGTACTTACAGGTCGTGCATGACAACTATC
TCCTGTATGTGTTTGCTCCAGACTGTGAGAGTCGGCAGCGCTGGGTGCTGACCCTTAAAGAAGAAACGAG
GAATAACAACAGCCTGGTATCCAAGTATCACCCTAATTTCTGGATGGATGGGCGGTGGAGGTGCTGCTCC
CAGCTGGAGAAGCCTGCTGTAGGCTGTGCTCCCTACGACCCATCCAAGAATGCTTCAAAGAAGCCTCTTC
CTCCTACTCCTGAAGACAACAGGCGGTCATTTCAGGAACCTGAAGAAACCCTGGTCATTGCCTTGTACGA
CTACCAAACCAACGACCCTCAGGAGCTCGCACTGCGGTGTGATGAAGAGTACTACCTGCTGGACAGCTCC
GAGATCCACTGGTGGAGGGTTCAAGACAAAAATGGGCATGAAGGATATGCACCAAGCAGTTACCTGGTAG
AAAAATCTCCAAATAACCTTGAAACCTATGAGTGGTACAATAAAAGCATCAGCCGCGACAAAGCTGAAAA
ACTTCTTTTGGACACAGGTAAAGAAGGAGCTTTCATGGTCCGAGATTCCAGGACACCCGGGACATACACA
GTCTCTGTTTTCACCAAGGCCATCATAAGTGAGAACCCCTGTATAAAACATTATCACATCAAAGAAACAA
ATGACAGCCCCAAGCGCTACTACGTGGCTGAGAAGTATGTGTTTGACTCCATCCCTCTCCTCATCCAGTA
TCACCAGTACAATGGAGGAGGTTTGGTCACTCGACTCCGCTATCCAGTTTGCTCCTGGAGACAAAAAGCC
CCTGTCACAGCAGGGCTAAGATATGGGAAGTGGGTGATCCAACCCTCAGAGCTAACGTTCGTGCAGGAGA
TTGGCAGCGGGCAGTTTGGGCTGGTGCATCTCGGCTACTGGCTCAACAAGGACAAGGTGGCCATCAAGAC
CATTCAGGAAGGGGCGATGTCAGAAGAAGACTTTATCGAGGAGGCGGAAGTCATGATGAAACTCTCTCAC
CCCAAACTGGTGCAGCTCTATGGGGTGTGCCTGGAGCAAGCCCCCATCTGCCTGGTGTTTGAGTTCATGG
AGCACGGCTGCCTGTCGGATTACCTTCGAAGTCAGCGGGGTCTCTTTGCTGCGGAGACCCTGCTGGGCAT
GTGCCTGGATGTGTGTGAGGGCATGGCCTACCTGGAAAAAGCTTGTGTCATCCACAGAGACCTGGCAGCC
AGAAACTGTTTGGTGGGAGAAAACCAGGTCATCAAGGTGTCCGACTTTGGGATGACAAGATTTGTCCTTG
ATGATCAATATACCAGCTCCACGGGCACCAAATTCCCAGTGAAGTGGGCATCCCCAGAGGTGTTCTCCTT
TAGTCGCTATAGCAGCAAGTCAGATGTGTGGTCGTTTGGTGTACTGATGTGGGAAGTCTTCAGTGAAGGC
AAAATCCCATACGAAAACCGAAGCAATTCCGAGGTCGTGGAAGATATCAGCACTGGCTTTCGGTTATACA
AGCCCCGCCTGGCCTCCTGTCATGTCTACCAGATCATGAATCATTGCTGGAAAGAGAAACCAGAAGACCG
GCCACCCTTCTCCCAGCTGTTAAGCCAGCTGGCTGAAATCGCAGAAGCTGGGCTTTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001281965
Insert Size 1878 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001281965.1, NP_001268894.1
RefSeq Size 4292 bp
RefSeq ORF 1878 bp
Locus ID 16428
UniProt ID Q03526
Gene Summary Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Phosphorylates TBX21 at 'Tyr-525' and mediates its interaction with GATA3 (PubMed:15662016).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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