Large2 (NM_001290775) Mouse Untagged Clone
CAT#: MC228506
Gyltl1b (untagged) - Mouse glycosyltransferase-like 1B (Gyltl1b), transcript variant 5
CNY 6,080.00
货期*
4周
规格
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
经常一起买 (3)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 5730485C17Rik; AI891893; Gyltl1b; Largel; mKIAA4105 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC228506 representing NM_001290775
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGCATGTGGCTATCGTGTGTGCGGGATACAACTCCAGCCGAGAGATTATTACCCTAACGAAGTCCC TGCTATTCTACAGGAAAAATCCGCTGCACCTCCACCTGATAACTGATGCCGTAGCCAGAAACATCCTGGA GACACTCTTCCGAACATGGATGGTGCCAGCGGTGGTGGTCAGCTTCTATGATGCGGAAGAACTCAAGCCC CTGGTCTCCTGGATCCCCAACAAACACTACTCTGGCCTCTATGGGCTAATGAAGCTAGTACTTCCCAGCA TCCTGCCTCCCAGCCTGGCCCGAGTCATCGTCCTGGATACCGACGTCACTTTCTCCTCTGACATTGTGGA GCTCTGGGCACTCTTTGATCATTTTTCTGACAAGCAGGTGGTCGGTCTCGTGGAGAACCAGAGCGACTGG TACCTGGGCAACCTCTGGAAGAACCATAGGCCCTGGCCTGCCTTGGGCAGGGGATTTAACACAGGTGTGA TCCTGCTGTGGCTGGACAGGCTCCAGCAAACTGGCTGGGAGCAGATGTGGAAGGTGACAGCCAAACGAGA GCTGCTCACTCTGATGGCTACTTCCTTGGCTGACCAGGACATCTTCAATGCGGTCATCAAGGAGCACCCC CATCTGGTGCACCCCCTGCCCTGTGTCTGGAACGTGCAGCTGTCAGACCACACTCGGGCTGAGCGCTGCT ACCTGGAAGCAGCTGACCTCAAAGTGATCCACTGGAATTCACCAAAGAAGCTTCGAGTGAAGAACAAGCA CGCAGAATTCTTCCGTAATCTGCACTTGACCTTTCTGGGGTATGATGGGAAGCTACTGCGAAGAGAGCTC TTTGGATGCCCCAACCAGTTCCCTCCTGGGGCCGAGCAGTTGCAACAGGCCCTAACACAGCTGGATGAGG AAGAGCCCTGCTTTGAGTTCCGCCAACAGCAGCTCACTGTGCACCGGGTGCACATCACCTTCCTGCCCCA CCAGCCGCCACCTCCCCAGCCTCACGATGTCACCTTGGTGGCCCAACTCTCTATGGACCGGCTGCAGATG CTGGAAGCCCTGTGCAGGCACTGGCCAGGCCCCATGAGCCTGGCCTTGTACCTGACAGATGAAGAGGCTC AACAATTTCTTCATTTTGTGGAAACGTCGCCAGTGCTCTCTATGAGGAAGGATGTGGCCTACCATGTAGT GTACCGGGACGGTCCACTCTATCCAGTCAACCAGCTCCGCAACGTGGCCTTGGCCCAGGCTCTCACACCC TACGTCTTCCTCAGTGATATTGACTTCTTACCTGCCTACTCCCTCTACGACTACCTCAGGGCTTCTATCG AGCAGCTGGAGCTGGACAGTCGGCGCAAGACTGCTTTGGTGGTGCCTGCATTTGAGACCCTACACTACCG GTTCAGCTTCCCAAACTCTAAGGCAGAGCTGTTGACGTTACTGGATGCCGGCTCCCTTCACACCTTTAGG TACCACGAGTGGCCACAGGGTCACTCATCCACAGACTATTCCCGCTGGCGGGAAGCCCAGGCACCATACA GTGTGCAGTGGTCAGCTGACTATGAACCCTACGTGGTGGTACCCCGTGACTGCCCCCGTTATGATCCTCG CTTTGTGGGATTTGGCTGGAACAAGGTGGCCCACATCATAGAGTTGGATGCTCAGGAATATGAATTCCTG GTACTTCCTGAGGCCTTCTCTATCCACTTGCCCCACGCTCCAAGTCTTGACATCTCCCGCTTCCGCTCCA GCCCCACCTACCGCAACTGTCTCCAGGCCCTCAAGGAAGAGTTCCACCAGGACTTGTCAAGGCGCTATGG GTCTGCAGCCCTGAAATACCTCACTGCCCTGCAGCAGGCCCGAAGTCGGGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001290775 |
Insert Size | 1875 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001290775.1, NP_001277704.1 |
RefSeq Size | 2673 bp |
RefSeq ORF | 1875 bp |
Locus ID | 228366 |
UniProt ID | Q5XPT3 |
Gene Summary | Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Has a higher activity toward alpha-dystroglycan than LARGE (PubMed:15958417).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) uses an alternate splice site in the 5' region, and it thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (4) is shorter at the N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR230717 | Gyltl1b (myc-DDK-tagged) - Mouse glycosyltransferase-like 1B (Gyltl1b), transcript variant 5 |
CNY 6,270.00 |
Customer
Reviews
Loading...