Atg16l1 (NM_001205391) Mouse Untagged Clone

CAT#: MC228505

Atg16l1 (untagged) - Mouse autophagy related 16-like 1 (S. cerevisiae) (Atg16l1), transcript variant 1



  "NM_001205391" in other vectors (1)

CNY 6,080.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Goat Anti-ATG16L1 Antibody
    • 100 ug

CNY 5,371.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1500009K01Rik; Apg16l; Atg16l; WDR30
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228505 representing NM_001205391
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGTCGGGCCTGCGCGCCGCAGACTTTCCCCGCTGGAAGCGTCACATCGCGGAGGAACTGAGGCGCC
GGGACCGACTGCAGAGGCAGGCGTTCGAGGAGATCATTCTGCAGTATACCAAGTTGCTGGAAAAGTCAGA
TCTTCATTCAGTATTGACCCAGAAACTACAAGCAGAAAAGCATGACATGCCAAATAGGCATGAAATAAGT
CCTGGACATGATGGTGCGTGGAATGATAGTCAACTACAAGAAATGGCCCAGTTGAGGATCAAACACCAGG
AAGAGCTGACCGAACTGCACAAGAAGCGTGGGGAGTTAGCTCAGTTGGTGATTGACCTGAACAACCAAAT
GCAGCAGAAGGACAAGGAGATACAGATGAATGAAGCAAAGATTTCGGAGTATTTACAGACCATCTCTGAC
CTGGAGACAAACTGCCTGGACCTGCGCACCAAACTGCAGGACCTCGAGGTAGCCAACCAGACCCTGAAGG
ATGAGTATGACGCCCTGCAGATTACTTTTACTGCCCTAGAAGAGAAACTGAGGAAAACTACTGAGGAGAA
CCAGGAACTGGTCACCAGATGGATGGCTGAGAAGGCCCAAGAAGCCAATCGCCTCAATGCAGAGAATGAG
AAGGACTCCAGGAGGCGTCAAGCACGGCTGCAGAAGGAGCTTGCAGAAGCAGCAAAGGAACCTCTACCTG
TTGAACAGGATGATGACATTGAAGTCATTGTGGATGAGACCTCAGACCACACAGAAGAGACCTCTCCCGT
CCGAGCTGTCAGCAGAGCAGCTACTAAGCGACTCTCGCAGCCTGCTGGAGGCCTTCTGGATTCTATCACT
AATATCTTTGGTCTGTCCGAATCTCCCCTTTTGGGACATCATTCTTCTGATGCTGCCAGGAGACGCTCTG
TCTCTTCCATCCCAGTCCCCCAGGATATCATGGACACTCATCCTGCTTCTGGTAAAGATGTGAGAGTCCC
AACTACTGCCTCGTATGTCTTCGATGCGCATGACGGAGAGGTCAACGCAGTGCAGTTCAGTCCAGGCTCC
CGGTTGCTGGCCACTGGAGGCATGGACCGCAGGGTGAAACTTTGGGAAGCATTCGGAGATAAATGTGAAT
TCAAGGGCTCCCTGTCTGGCAGTAATGCTGGAATTACAAGCATTGAATTTGATAGTGCTGGAGCTTACCT
ATTAGCAGCTTCAAATGATTTTGCAAGCCGAATCTGGACTGTGGATGATTATCGATTACGGCACACACTC
ACAGGCCACAGCGGGAAAGTCCTCTCTGCCAAGTTCCTGCTGGACAATGCACGGATTGTCTCAGGAAGTC
ACGACCGGACCCTCAAACTCTGGGATCTCCGCAGCAAAGTCTGCATAAAAACAGTGTTTGCAGGATCCAG
CTGCAATGACATTGTTTGCACTGAACAATGTGTAATGAGTGGACATTTTGACAAGAAAATTCGTTTCTGG
GATATCCGGTCAGAGAGTGTGGTCCGAGAGATGGAACTGTTAGGGAAGATCACTGCTCTGGACCTAAACC
CTGAGAGAACTGAGCTCCTGAGCTGCTCCCGTGATGACCTGCTAAAAGTCATCGACCTCCGGACAAATGC
AGTCAAACAGACATTCAGTGCGCCTGGATTCAAATGCGGCTCTGACTGGACCCGGGTTGTCTTCAGCCCT
GATGGCAGTTACGTGGCAGCAGGCTCAGCCGAGGGTTCTCTTTATGTCTGGAGTGTGCTGACAGGGAAAG
TGGAGAAGGTTCTTTCAAAACAGCACAGCTCTTCTATCAATGCGGTGGCGTGGGCCCCCTCGGGCTTACA
TGTTGTCAGTGTGGACAAAGGAAGCAGAGCTGTGCTGTGGGCACAGCCTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001205391
Insert Size 1872 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001205391.1, NP_001192320.1
RefSeq Size 3197 bp
RefSeq ORF 1872 bp
Locus ID 77040
UniProt ID Q8C0J2
Gene Summary Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane (PubMed:18849966, PubMed:12665549, PubMed:24954904, PubMed:24553140, PubMed:23392225). Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (By similarity). Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response (PubMed:24238340). Instead, promotes with NOD2 an autophagy-dependent antibacterial pathway. Plays a role in regulating morphology and function of Paneth cell.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1, also known as Apg16Lgamma) represents the longest transcript and encodes the longest isoform (1, also known as Apg16Lgamma). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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