Atg9a (NM_001288613) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Apg9l1; Atg9; Atg9l1; AU019532
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228500 representing NM_001288613 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGCCCTGGTGAACAAATCCCTCCTGCCTCTGCGCTTCCGTCTGCCCGGCCTCGGAGAGGTTGTCT TCTTCACCCGTGGCCTCAAGTACAACTTTGAGCTCATCCTCTTCTGGGGACCCGGCTCTCTGTTTCTCAA TGAGTGGAGCCTCAAGGCCGAGTACAAACGTGGAGGGCAACGGCTAGAGCTGGCCCAGCGTCTCAGCAAC CGCATCTTGTGGATTGGCATCGCCAACTTCCTGCTGTGTCCCCTCATCCTCATCTGGCAGATCCTCTATG CCTTCTTCAGCTATGCCGAGGTGCTGAAGAGAGAGCCGGGGGCCCTGGGAGCGCGTTGCTGGTCACTCTA TGGCCGTTGTTACCTCCGCCACTTCAATGAGCTGGAGCATGAGCTGCAGTCCCGCCTCAACCGAGGCTAC AAGCCCGCCTCCAAGTACATGAATTGCTTCTTGTCACCGCTGCTGACTCTGCTGGCCAAGAATGGTGCCT TCTTCGCTGGCTCTATCCTGGCTGTGCTTATTGCCCTCACCATCTATGATGAAGATGTGTTAGCTGTGGA ACACGTCCTCACCACGGTCACCCTCCTGGGAGTCACGGTGACTGTGTGCAGGTCCTTCATCCCAGACCAG CACATGGTGTTCTGCCCCGAGCAGCTGCTCCGAGTGATTCTTGCACACATCCACTACATGCCTGACCACT GGCAGGGTAATGCCCACCGCTCGCAGACCCGGGACGAGTTTGCCCAGCTCTTCCAGTACAAGGCAGTGTT CATCTTGGAGGAGTTGCTGAGTCCCATCGTCACACCCCTCATTCTCATCTTCTGCCTCCGCCCTCGGGCC CTGGAGATCATAGACTTCTTCCGCAACTTTACGGTCGAGGTCGTGGGTGTGGGAGACACCTGCTCCTTTG CTCAGATGGACGTTCGCCAGCATGGCCATCCTCAGTGGCTGTCTGGAGGGCAGACAGAGGCCTCAGTGTA CCAGCAAGCCGAGGACGGGAAGACCGAGTTGTCGCTCATGCACTTTGCCATCACCAATCCCGGCTGGCAG CCCCCTCGTGAGAGCACAGCTTTCCTGGGCTTCCTCAAGGAGCAGGTGCAGCGAGATGGAGCAGCTGCTG GCCTGGCCCAGGGTGGCCTGCTCCCCGAGAATGCCCTCTTCACATCCATCCAGTCCTTACAGTCTGAGTC CGAGCCACTGAGCCTTATTGCAAATGTGGTAGCAGGCTCATCCTGCCGAGGACCCTCACTGTCCAGAGAC CTGCAGGGCTCCAGGCACAGGGCTGATGTTGCTTCTGCCCTTCGATCCTTCTCCCCTCTGCAGCCTGGAG CGGCCCCTCAAGGCCGGGTTCCCAGTACCATGACAGGCTCTGGAGTGGATGCCAGGACAGCCAGCTCTGG GAGTAGCGTGTGGGAAGGACAGCTGCAGAGCCTGGTGCTGTCCGAATACGCGTCCACCGAGATGAGCCTG CACGCCCTGTACATGCACCAGCTCCACAAGCAGCAGACCCAGGCTGAGCCCGAGCGGCATGTGTGGCACC GCCGGGAGAGTGATGAGAGTGGAGAGAGTGCCCCTGAAGAGGGGGGAGAGGGTGCCCGGGCCCCCCAACC CATCCCCCGCTCGGCCAGCTATCCCTGTGCTACACCCCGGCCTGGAGCACCTGAGACCACCGCCCTGCAT GGGGGCTTCCAGAGGCGCTACGGGGGCATCACAGATCCTGGCACAGTGCCCCGTGGCCCCTCTCACTTCT CCAGGCTGCCCCTGGGAGGATGGGCAGAAGATGGCCAGCCAGCATCAAGGCACCCAGAGCCGGTGCCAGA GGAGGGCTCAGAGGATGAACTCCCCCCTCAAGTGCACAAGGTATAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001288613
Insert Size	1866 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001288613.1, NP_001275542.1
RefSeq Size	4104 bp
RefSeq ORF	1866 bp
Locus ID	245860
Gene Summary	Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H (By similarity). Required for carbonyl cyanide m-chlorophenylhydrazone (CCCP)-induced ATG8 family proteins lipidation, a key autophagy step.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate splice junction at the 5' end of an exon and initiates translation at an alternate start codon, compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a.

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