Tiam2 (NM_001286757) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3000002F19Rik; mKIAA2016; STEF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228494 representing NM_001286757 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGCACCAACAGAGAGCCATGACCCACCTCCCAGGCCTCTGGCTCGTCACCTCTCAGATGCAGATC GCCTCCGGAAAGTCATCCAGGAGCTTGTAGACACAGAGAAGTCTTACGTGAAGGATCTGAGCTGCCTCTT TGAACTATACTTGGAGCCACTTCAGAATGAGACCTTTCTTACCCAAGATGAGATGGAGTCACTTTTTGGG AGCCTGCCAGAGATGCTGGAGTTTCAAAAGGTGTTCCTGGAGACGTTGGAGGATGCGATCTCCGCTTCCT CGGACTTTAGTGTCCTGGAAACCCCCTCACAGTTTCGAAAATTGCTGTTCTCCCTTGGAGGTTCTTTCCT CTACTATGCGGATCACTTTAAGCTATACAGTGGGTTCTGTGCCAACCACATTAAAGTACAGAGGGTTCTA GAGCGAGCTAAAACGGACAAGGCCTTCAAGGCTTTTCTGGATGCCCGAAATCCCACCAAGCAGCACTCCT CCACGCTGGAGTCCTATCTCATCAAGCCTGTTCAGAGAGTGCTCAAGTATCCTCTGCTTCTCAAGGAGCT AGTGTCACTGACTGACCATGAGAGTGAAGAACACTATCACCTGACAGAAGCACTAAAGGCCATGGAAAAA GTCGCCAGTCACATCAATGAGATGCAGAAGATCTACGAGGATTACGGGATGGTGTTTGACCAGCTGGTGG CAGAGCAGAGTGGCACAGAGAAGGAAGTGACAGAGCTGTCCATGGGGGAACTTCTGATGCACTCTACAGT TTCCTGGTTGAATCCGTTCCTGTCTCTAGGAAAAGCCAGGAAGGACATTGAGCTCACAGTATTTGTTTTT AAGAGAGCTGTCATACTGGTTTATAAAGAAAACTGCAAGCTGAAAAAGAAACTGCCCTCGAATTCCCGGC CTGCTCACAACTCTGCTGACTTGGATCCATTTAAATTCCGCTGGTTGATTCCCATATCTGCGCTTCAAGT TAGACTGGGGAACACGGCAGGGACTGAAAATAATTCCACGTGGGAGCTGATTCATACCAAGTCGGAAATT GAAGGACGGCCAGAAACCATCTTTCAACTGTGCTGCAGTGACAGCGAGAACAAAACCAGCATTGTTAAGG TGATTCGTTCTATTCTGAGAGAGAACTTCCGGCGCCACATAAAGTGTGAGCTGCCACTGGAGAAGACGTG TAAGGACCGGCTAGTACCTCTTAAGAACCGAGTTCCTGTTTCAGCCAAATTAGCCTCGTCCAGGTCGTTG AAGGGCCTCAGAACATCCTCCAGCAGCGAGTGGCCCAGCGAGCCCAGCAAGGGCAACTCACTGGACTCAG ATGAGTGCAGCCTGAGCAGTGGCACCCAGAGTAGCGGCTGCCCCGTAGCCGAGAGCAGGCGAGACTCTAA GAGCACCGAGCTGGAGAAAGACGCTCAGGAGGGCCTGGCGGAGTTTCCAGATGGTCTTATCAAAGAAAGC GACATTCTGAGTGATGAAGATGAGGACTTCCACCACCCTCTGAAACAGGGTAGCCCTACTAAGGACATTG AGATTCAGTTCCAGAGACTGAAAATCTCTGAGGAATCCGACGTGCACCCAGTTGGGCAGCAGCCTCTCAC AGAGTCAGGTGAACAGCCCAAGCTGGTCAGGGGCCATTTTTGCCCCATTAAACGGAAAGCAAACAGCACC AAGAGGGGCAGAGGAACTTTGCTCAAGGCGCAGACTCGTCACCAGTCCCTGGACAGCCACCCAGAAACTG CCAGCATTGATCTAAACTTGGTCCTGGAGAGAGAATTCAGTGTCCAGAGCTTAACTTCAGTCGTCAATGA GGAGGGTTTTTATGAAACACAGAGCCATGGCAAATCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286757
Insert Size	1860 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286757.1, NP_001273686.1
RefSeq Size	3186 bp
RefSeq ORF	1860 bp
Locus ID	24001
UniProt ID	Q6ZPF3
Gene Summary	Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Activates specifically RAC1, but not CDC42 and RHOA. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.

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