Usp2 (NM_198092) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	B930035K21Rik; Ubp41
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228489 representing NM_198092 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCAGCTCTCCTCCACCCTGAAGCGCTATACAGAATCGTCCCGCTACACAGATGCCCCCTATGCCA AACCGGGCTATGGCACCTACACCCCTTCTTCCTATGGGGCCAACCTGGCCGCCTCCTTCCTGGAGAAGGA AAAACTTGGTTTCAAGCCGGTCTCCCCCACCAGCTTCCTCCCACGGCCCCGCACCTACGGCCCCTCCTCC ATTCTGGACTGTGACAGGGGCCGCCCCCTGCTGAGATCTGACATCATTGGGAGTAGTAAGCGGTCAGAGA GCCAGACCCGTGGCAATGAAAGGCCCTCGGGCAGTGGACTCAACGGAGGCAGTGGATTTTCTTATGGAGT TTCCAGCAACTCCCTCAGCTACCTGCCCATGAATGCCAGAGACCAGGGGGTGACCCTGAGCCAGAAGAAA TCGAACAGCCAATCAGACCTGGCCCGGGATTTCTCCAGCCTCCGGACTTCAGATGGCTACCGGACTTCAG ATGGCTACCGGACCTCAGAGGGCTTTAGGATAGACCCTGGAAACCTGGGTCGCAGCCCCATGCTGGCCCG CACGCGTAAGGAGCTCTGTGCCCTGCAGGGCCTCTACCAAGCAGCCAGCCGTTCTGAGTACCTAACAGAC TATCTGGAGAACTATGGTCGAAAGGGCAGTGCACCACAGGTGCTCACGCAAGCCCCTCCTCCCTCCCGAG TCCCTGAAGTCCTCAGTCCCACCTACCGACCAAGTGGCCGTTACACACTGTGGGAGAAGAGCAAGGGCCA GGCCTCTGGGCCCAGCCGCTCCAGTTCTCCAGGGCGAGACACCATGAATTCAAAGAGTGCCCAGGGTCTG GCTGGTCTTCGAAACCTTGGGAACACGTGCTTCATGAACTCAATTCTTCAGTGCCTGAGCAACACCCGAG AGCTGAGAGATTACTGCCTCCAGAGGCTGTACATGCGGGACCTCGGCCACACCAGCAGCGCTCACACGGC CCTCATGGAAGAGTTTGCAAAACTAATCCAGACCATATGGACGTCGTCCCCCAATGATGTGGTGAGCCCA TCTGAGTTCAAGACCCAGATCCAGAGATATGCGCCACGCTTCATGGGCTATAATCAGCAGGATGCTCAGG AATTCCTTCGTTTCCTTCTGGATGGTCTCCACAATGAGGTGAACCGGGTGGCAGCAAGGCCTAAGGCCAG CCCTGAGACCCTTGATCATCTCCCTGATGAAGAAAAGGGGCGACAGATGTGGAGGAAGTATCTGGAAAGG GAAGACAGTCGGATTGGGGATCTCTTCGTTGGGCAGCTGAAGAGCTCCCTCACATGCACCGATTGTGGCT ACTGCTCTACAGTCTTCGATCCCTTCTGGGATCTCTCGTTGCCCATCGCAAAGAGAGGTTACCCTGAGGT GACGTTAATGGATTGTATGAGGCTCTTCACCAAAGAGGACATATTGGATGGTGATGAGAAGCCAACTTGC TGCCGCTGCCGAGCCAGAAAACGATGCATAAAAAAGTTCTCTGTCCAGAGGTTCCCAAAGATCTTGGTGC TCCACCTGAAGCGATTCTCAGAATCCAGGATACGAACCAGCAAGCTCACAACATTTGTGAATTTCCCACT AAGAGACCTGGACTTGAGAGAATTTGCTTCAGAAAACACCAACCATGCTGTTTACAACCTGTATGCTGTG TCCAATCACTCCGGAACCACCATGGGAGGCCACTATACAGCCTACTGCCGAAGTCCGGTTACAGGCGAAT GGCACACTTTCAATGATTCCAGTGTCACACCCATGTCCTCCAGCCAAGTGCGCACCAGCGACGCCTATTT GCTCTTCTATGAACTGGCCAGTCCACCCTCCCGTATGTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_198092
Insert Size	1860 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_198092.2, NP_932760.2
RefSeq Size	3882 bp
RefSeq ORF	1860 bp
Locus ID	53376
UniProt ID	O88623
Gene Summary	Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1 (By similarity). Isoform 1 and isoform 2 possess both ubiquitin-specific peptidase and isopeptidase activities (By similarity). Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity (By similarity). Has no deubiquitinase activity against p53/TP53 (By similarity). Prevents MDM2-mediated degradation of MDM4 (By similarity). Plays a role in the G1/S cell-cycle progression in normal and cancer cells (By similarity). Plays a role in the regulation of myogenic differentiation of embryonic muscle cells (By similarity). Regulates the circadian clock by modulating its intrinsic circadian rhythm and its capacity to respond to external cues (PubMed:23213472, PubMed:25238854, PubMed:26756164). Associates with clock proteins and deubiquitinates core clock component PER1 but does not affect its overall stability (PubMed:23213472). Regulates the nucleocytoplasmic shuttling and nuclear retention of PER1 and its repressive role on the clock transcription factors CLOCK and ARNTL/BMAL1 (PubMed:25238854).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) represents the longest transcript and encodes the longer protein (isoform Usp2-69). Variants 1 and 3 encode the same protein.

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