Dtx2 (NM_001256096) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610524D08Rik; AA408415; AU022494; Deltex2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228487 representing NM_001256096 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATGGCCCCAAGCTCGTCTCTGCCGCAGGTGTACCCTAGCCACGTGGTGGTGGCTGTGTGGGAAT GGCAGGATGGGCTTGGCATTTGGCACCCCTACAGTGCCACTGTCTGCTCCTTCATTGAGCAGCATTTTGT CCGGCAGAGGGGCCAGCATTTTGGACTGGGAAGCCTGGCCCACAGCATCCCCTTAGGCCAAGCTGACCCC TCACTGGCCCCTTACATCATCGACCTCCCCAGCTGGACTCAGTTTCGCCAGAACACTGGCACCATGAGGT CTGTGCGCAGACACCTGTTCTCACAGAATTCTGCCCCAGGCCAGGGCATCGTCTGGGAGTGGCTGGGCGA TGACGGATCCTGGGTAGCCTATGAAGCTAGAATCTGTGACTATCTGGAACAGCAAGTGGCCCGGGGCATC CAGGTCGTGGACTTGGCACCACTGGGGTATAACTATACTGTCAACTATGCCACCCTAACCCAAACCAACA AGACTTCCAGCTTCTGCCGGAGTGTGCGACGCCAAGTGGGGCCAGTTTACCCAGTGACTTCAGACATCGC TGTTCCACGCCAAATGGGACTTATCTGCTTTTGCCAACAGTGCCTCCATGGTAGCGGAACTGGCCCTGTG TCGGGCCGCTACCGCCACTCCATGACCAACCTGCCTGCATATCCTGCCCCCCAAGCACCCCACCGGACCA CCACTGTCTCTGGGGCCCACCAGGCCTTTGCCCCATACAATAAACCTTCACTGTCTGGGGCCAGATCTGC ACCAAGGTTGAACACCACCAACCCCTGGGCTGCAGCACCTCCTGTTGCAGGCAACCAGTCCCTGTTCCAC TCCAGCCTCTCCCACCTGGGGCCTCAGCTCCTGCCCTCAGGACCGTCCACCTCCAGTGGAGCCAGTGCCT CCTTCCCCAGCGGGCCCTCCTCCAGCAGCCCAGGGAGCGCCCCCACCACTGTGCCCGTGCAGATGCCAAA GGCCAGCAGGGTCCAGCAGGCGCTTGCAGGCATGACGAGTGTGCTGTCAGCCATTGGACTCCCTGTGTGT CTTAGCCGTGCACCCCGGCCCACCGGCCCTCCCGCCTCCCGTCCGGCCTCTAAAAGTCACAGCTCAGTTA AGAGGCTGAGGAAAATGTCCGTAAAAGAAGGGGCGCCTAAGCCCGAGCCGGAGCAGGTGATCAGAAAGTA CACCGAGGAGCTGAAAGTGGCCCCTGAAGAGGACTGCATTATCTGTATGGAGAAACTGGCTGTGGCTTCT GGGTACAGTGACATGACTGACAGCAAGGCCCTCGGGCCCATGGTCGTGGGCCGCCTCACCAAGTGCAGCC ATGCCTTCCACCTGCTGTGCCTGCTGGCCATGTATTGCAACGGGAACAAGGACGGGAGTCTTCAGTGTCC GTCGTGCAAAACCATCTATGGGGAGAAGACTGGGACGCAGCCCTGGGGGAAGATGGAGGTGTTCAGGTTC CAAATGTCCCTCCCAGGCCATGAAGACTGTGGGACTATACTCATTGTTTACAACATCCCTCATGGCATCC AGGGACCAGAGCACCCCAGCCCTGGGAAGCCATTCACTGCCCGGGGCTTTCCCCGGCAATGCTACCTGCC AGATAGCCCTCAGGGCCGCAAGGTCCTGGAGCTCCTGAAGGTGGCCTGGAAGAGGCGACTCATTTTTACC GTGGGCACTTCCAGCACCACAGGTGAGACCGACACCGTGGTGTGGAACGAGATCCACCACAAGACCGAGA TGGACCGCAACGTGACAGGCCACGGCTATCCGGACCCCAACTACCTGCAGAATGTGCTGGCTGAGCTCGC TGCCCAGGGAGTGACCGAAGACTGCCTGGAACAGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256096
Insert Size	1860 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256096.1, NP_001243025.1
RefSeq Size	2658 bp
RefSeq ORF	1860 bp
Locus ID	74198
UniProt ID	Q8R3P2
Gene Summary	Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Functions as a ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1).

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